Distribution and co-occurrence of antibiotic and metal resistance genes in biofilms of an anthropogenically impacted stream

Roberto, Alescia A.; Gray, Jonathon B. Van; Engohang‐Ndong, Jean; Leff, Laura Gunn

doi:10.1016/j.scitotenv.2019.06.053

Cited by 49 publications

(33 citation statements)

References 105 publications

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“…Aquatic environments such as wastewater and river water are affected by physicochemical factors that contribute to changes in these environments [ 77 ]. Zieliński et al [ 78 ] focused on the epidemiological threat posed by antibiotic resistance determinants to WWTP employees and the environment.…”

Section: Resultsmentioning

confidence: 99%

The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study

et al. 2022

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The aim of this study was to quantify ESKAPEE bacteria, genes encoding resistance to antibiotics targeting this group of pathogens, as well as integrase genes in municipal wastewater and river water. Environmental DNA was extracted from the collected samples and used in deep sequencing with the Illumina TruSeq kit. The abundance of bacterial genera and species belonging to the ESKAPEE group, 400 ARGs associated with this microbial group, and three classes of integrase genes were determined. A taxonomic analysis revealed that Acinetobacter was the dominant bacterial genus, whereas Acinetobacter baumannii and Escherichia coli were the dominant bacterial species. The analyzed samples were characterized by the highest concentrations of the following ARGs: blaGES, blaOXA-58, blaTEM, qnrB, and qnrS. Acinetobacter baumannii, E. coli, and genes encoding resistance to β-lactams (blaVEB-1, blaIMP-1, blaGES, blaOXA-58, blaCTX-M, and blaTEM) and fluoroquinolones (qnrS) were detected in samples of river water collected downstream from the wastewater discharge point. The correlation analysis revealed a strong relationship between A. baumannii (bacterial species regarded as an emerging human pathogen) and genes encoding resistance to all tested groups of antimicrobials. The transmission of the studied bacteria (in particular A. baumannii) and ARGs to the aquatic environment poses a public health risk.

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Section: Resultsmentioning

confidence: 99%

The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study

et al. 2022

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“…Compared with a study in the Yarlung Tsangpo River [ 43 ], ARG subtypes in this study were more diverse, but the number of ARG subtypes in this study was lower than in the Chaobai River [ 44 ]. Variations in ARG subtype number may be related to differences in human activities, physicochemical parameters, bacterial community, MGEs, and different sample types studied [ 22 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Frequency of occurrence and habitat selection shape the spatial variation in the antibiotic resistome in riverine ecosystems in eastern China

Jiang

Chen

Grossart

et al. 2022

Environmental Microbiome

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Background Riverine ecosystems are one of the most important reservoirs of antibiotic resistance genes (ARGs) in the environment, but the occurrence and controlling factors of ARG distribution in different habitats of riverine ecosystems remain poorly understood. In this study, a metagenomic approach was used to characterize ARG types and their abundance in different habitats (rhizosphere soil, surface bulk soil, bottom bulk soil, and sediment) of riverine ecosystems in eastern China. Sampling sites were located along different rivers of eastern China, which are geographically isolated. Differences in bacterial communities, mobile genetic elements (MGEs), pattern and intensity of human activities, climate, and other environmental factors at the sampling sites and habitats were expected to affect ARG occurrence. Results ARGs were observed with high variations in diversity (44–206 subtypes) and abundance (6.85–105.68 ×/Gb). There were significant south-north differences in ARG occurrence in the same habitat, except for surface bulk soil. And the significant difference was found in ARGs among four southern habitats. South–north differences in ARGs of the same habitat were mainly attributed to the combination of different occurrence frequencies and habitat selections of ARGs. Differences in ARG profiles among the four habitats in the south and the north were both mainly attributed to the different occurrence frequencies of ARGs. Bacterial communities and MGEs (Mobile genetic elements) could account for the observed variance in the resistome of riverine ecosystems across eastern China. The co-occurrences of specific ARGs with bacterial communities and MGEs were more frequent at the northern sampling sites than in the south, and co-occurrence patterns (i.e. ARGs and bacterial communities or ARGs and MGEs) varied between the habitats. Moreover, building land in all habitats, except bulk soils, showed significant positive correlations with ARG abundance. Conclusion This study reveals a high variance in the resistome of riverine ecosystems in eastern China and its controlling factors. We appeal to the importance of assessment of ARGs in the riverine ecosystem and the need for future prevention and intervention of ARG spread.

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“…Moreover, Alteromonas was reported to be a dominant colonizer of copper-based SPCs after 1-4 weeks in marinas from two dissimilar environments, i.e., Singapore (Chen et al, 2013) and the Swedish West coast (Flach et al, 2017). Two hypotheses are generally proposed to explain such a metal resistance/tolerance mechanism: the occurrence of metal resistance genes (MRG) (Flach et al, 2017;Roberto et al, 2019;Catao et al, 2021) and the exopolymeric substances production (EPS) (Flemming et al, 2016). The former was recently demonstrated for several MRG, including copper specific (cusA, copA, and CueO) or not (czcA and pbrT), on substrates including copper-released coatings in the Bay of Toulon but also in a port of the French Atlantic coast in Brittany (Lorient) and in the tropical Indian ocean (Reunion island).…”

Section: Alteromonas a Pioneer And Copper Resistant Prokaryote Genusmentioning

confidence: 99%

Surface Characteristics Together With Environmental Conditions Shape Marine Biofilm Dynamics in Coastal NW Mediterranean Locations

et al. 2022

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Microbial colonization of artificial substrates in coastal areas, which concerns hull ships, sensors as well as plastic debris, is of huge significance to attain a rational environmental management. Some surface and environmental drivers of biofilm development have previously been described but their relative impact on the formation of biofilms remains unknown while crucial. Especially, there is no evidence of the relative importance of physical surface properties (wettability, roughness, smoothness) compared to seawater characteristics in driving biofilm abundance and diversity. In addition, few studies have considered the temporal evolution of this complex form of colonization, which often prevent to globally understand the process. Using experimental facilities in two Mediterrranean locations, a multidisciplinary approach including surface characterizations as well as seawaterquality analyses, flow cytometry and 16S rDNA metabarcoding, allowed for the identification of the main drivers of colonization for two antifouling (AF) coatings. One AF coating released copper (SPC1) while the other limit colonization thanks to physical properties, namely a low surface energy, roughness and smoothness (FRC1). Results were obtained over 75 days and compared to a control surface (PVC). Biofilm development was observed on all surfaces, with increasing density from AF coatings to PVC. Pionneer bacteria were dissimilar within all three surface types, however, communities observed on FRC1 converged toward PVC ones overtime, whereas SPC1 communities remained highly specific. A remarkably low and unique diversity was found on SPC1 during the experiment as Alteromonas accounted for more than 90% of the community colonizing this substrate until 12 days, and remained one of the co-dominant taxa of mature biofilms. Moreover, clear differences were found between geographical locations. Low nutrients and higher hydrodymanics in Banyuls bay resulted in less dense biofilms overall compared to Toulon, but also in a the slower dynamic of biofilm formation. This is illustrated by the persistence of pioneer Alteromonas but also Hyphomonadacae after 75 days on SPC1. We concluded that, even if local environmental conditions influenced the composition of biofilm communities, particular physical features may control the biofilm density but not the diversity, while copper releasing coating controlled both. In addition, it is evident from these results that sequential biofilm dynamics should carefully be considered as initial processes of formation differed from the long-term ones.

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Distribution and co-occurrence of antibiotic and metal resistance genes in biofilms of an anthropogenically impacted stream

Cited by 49 publications

References 105 publications

The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study

The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study

Frequency of occurrence and habitat selection shape the spatial variation in the antibiotic resistome in riverine ecosystems in eastern China

Surface Characteristics Together With Environmental Conditions Shape Marine Biofilm Dynamics in Coastal NW Mediterranean Locations

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