Distribution and genetic diversity of begomoviruses infecting tomato and pepper plants in the Philippines

Abstract

Begomoviruses were found to be the major viruses infecting tomato plants in the Philippines based on the surveys conducted from 2005 to 2006. Pepper-infecting begomoviruses were also detected. Isolates of four distinct begomovirus species, Ageratum yellow vein virus (AYVV), Tomato leaf curl Cebu virus (ToLCCeV), Tomato leaf curl Mindanao virus (ToLCMiV) and Tomato leaf curl Philippines virus (ToLCPV), were characterised at the DNA sequence level by comparing 20 DNA-As from tomato samples comprising 13 from Luzon, 2 from Cebu and 5 from Mindanao Islands, along with 3 DNA-As from pepper samples, 1 each from Luzon, Cebu and Mindanao Islands. Two of these species (ToLCCeV and ToLCMiV) were distinct novel begomoviruses, while AYVV was detected for the first time in the Philippines. By geographic distribution, two tomato begomoviruses (ToLCPV and ToLCCeV) were detected in Luzon and Cebu Islands. The ToLCMiV was also detected in Luzon Island. The three tomato begomoviruses, AYVV, ToLCCeV and ToLCMiV, were detected in Mindanao Island. A ToLCPV isolate infecting pepper was also detected in Luzon Island, while ToLCCeV was detected in pepper samples from Cebu and Mindanao Islands. The diversity of viruses and their distinct geographic distribution need to be taken into consideration in the development and deployment of resistance against begomoviruses in the Philippines. Strategies for the use of post-transcriptional gene silencing for the control of tomato-infecting begomoviruses in the Philippines are discussed

Molecular Characterization of Begomoviruses Infecting Sauropus androgynus in Thailand

“…; Tsai et al. ). The isolates of begomoviruses infecting sauropus identified as being in the AYVV cluster were diverse, distributed in a distinct branch (SP1 isolate) and in three subclusters (SPa, SPb and SPd) of the AYVV cluster.…”

“…; Tsai et al. ). The recombination analysis suggested likely major and minor contributors to the recombinant isolates from among the sequences available from this study and from GenBank, and thus, the major contributor of isolate SP1 is assessed to be AYVV‐[G68] (Guangxi, China) and the major contributor of the SPd cluster isolates to be AYVV‐[G129] (Guangxi, China).…”

“…Similarly, AYVV isolates were first identified from Ageratum spp. (Tan et al 1995;Green et al 2005), but also have been reported to infect economically important tomato crops in Indonesia, Japan and the Philippines (Kon et al 2006;Andou et al 2010;Tsai et al 2011b). The isolates of begomoviruses infecting sauropus identified as being in the AYVV cluster were diverse, distributed in a distinct branch (SP1 isolate) and in three subclusters (SPa, SPb and SPd) of the AYVV cluster.…”

Molecular characterization of genetic diversity of tomato (Solanum lycopersicum L.) begomovirus in Togo

“…Author(s) agree that this article remains permanently open access under the terms of the Creative Commons Attribution License 4.0 International License (family Geminiviridae) have been identified as naturally infecting tomato (Solanum lycopersicum L.) in different parts of the world (Tsai et al, 2011;Van Brunschot et al, 2013) and even in some places, these diseases have eliminated the tomato as an economically viable crop (Zhou et al, 2008;Hanssen et al, 2010).…”

Genetic Diversity of Tomato (<i>Solanum lycopersicum</i> L.) Begomovirus in Togo

“…In particular, approximately 70 species of the genus Begomovirus (family Geminiviridae) have been identified as naturally infecting tomato (Solanum lycopersicum L.) in different parts of the world [2] [3] and even in some places, these diseases have eliminated the tomato as an economically viable crop [4] [5].…”