Distribution and genetic heterogeneity of Puumala virus in Sweden

Hörling, Jan; Lundkvist, Åke; Jaarola, Maarit; Plyusnin, Alexander; Tegelström, Håkan; Persson, Katarina; Lehväslaiho, Heikki; Hörnfeldt, Birger; Vaheri, Antti; Niklasson, Bo

doi:10.1099/0022-1317-77-10-2555

Cited by 54 publications

(51 citation statements)

References 36 publications

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“…Genetic analyses of strains from the Baltic countries, Austria, Slovakia, France, Belgium, Norway, Denmark, Sweden, Finland, Germany, and Russia have shown PUUV to be the most variable of the hantavirus species; genetic diversity at the nucleotide level reaches 20% and 17% for the coding regions of the M-and S-segments, respectively, and reaches even higher values (37% and 30%) for their 3'-noncoding regions [6,8,17,18,29,32].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Study of Hantavirus Infection in the Samara Region of European Russia

et al. 2009

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ABSTRACT. European Russia is a highly endemic area of hemorrhagic fever with renal syndrome (HFRS), a rodent-borne zoonotic disease, caused by hantaviruses. In total, 145 small mammals of four species (Myodes glareolus, Apodemus flavicollis, A. agrarius, and A. uralensis) were trapped in the Samara region of European Russia in August 2005 and examined for the presence of hantavirus (HV). Anti-HV antibodies were found in six of 68 (8.8%) M. glareolus and in one of 19 (5.3%) A. flavicollis by indirect immunofluorescent antibody assay (IFA). The Puumala virus (PUUV), which is one of the hantavirus species, was detected in the lungs of seven M. glareolus by RT-PCR. The virus S-segment was extremely similar (96.2% to 99.3%) to the sequence found in a fatal case of HFRS in the Samara region. Phylogenetic analyses of S and M segments showed that the Samara PUUVs form a cluster within the Russian Volga lineage and apparently differ from other European PUUVs. Anti-PUUV antibodies were found in blood sera from seven HFRS patients and from one undiagnosed patient from the Samara region, using IFA and an enzyme-linked immunosorbent assay (ELISA). These data suggest that the bank vole M. glareolus is a primary natural reservoir and vector for PUUV, which is the main causative agent of HFRS in humans in the Samara region.KEY WORDS: epidemiology, hantavirus, hemorrhagic fever with renal syndrome, Puumala virus, rodents.J. Vet. Med. Sci. 71(12): 1569-1578, 2009 Hantaviruses belong to the genus Hantavirus, within the family Bunyaviridae, and cause two forms of human illness. These zoonoses include hemorrhagic fever with renal syndrome (HFRS), which occurs in Asia and Europe and is caused by the Hantaan virus (HTNV), Seoul virus (SEOV), Puumala virus (PUUV), Dobrava-Belgrade virus (DOBV), and Amur virus (AMRV). The second form of illness is hantavirus (cardio) pulmonary syndrome (HPS), which occurs in the Americas and is caused by the Sin Nombre virus, Andes virus, and other hantaviruses [13,28,33]. Hantaviruses are transmitted by aerosolized excreta from their natural hosts, mainly rodents of the family Muridae. Particular hantavirus species are usually harbored by a single rodent species, or by a few closely related rodent species, which indicates co-evolution and co-speciation of hantaviruses with their hosts. The virus genome contains three segments of negative-stranded RNA: the large (L) segment encodes a viral RNA-dependent RNA polymerase, the medium (M) segment encodes a glycoprotein precursor, and the small (S) segment encodes a nucleocapsid protein (NP) [34].In

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Section: Discussionmentioning

confidence: 99%

Epidemiological Study of Hantavirus Infection in the Samara Region of European Russia

et al. 2009

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“…Total IgG responses to PUUV were measured by PUUV native N ELISA, essentially as described previously (16). Rabbit anti-PUUV serum diluted 1:400 was adsorbed to Maxisorp plates overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Cross-Protection against Challenge with Puumala Virus after Immunization with Nucleocapsid Proteins from Different Hantaviruses

Nicacio

Valle

Padula

et al. 2002

J Virol

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Hantaviruses are rodent-borne agents that cause hemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome in humans. The nucleocapsid protein (N) is relatively conserved among hantaviruses and highly immunogenic in both laboratory animals and humans, and it has been shown to induce efficient protective immunity in animal models. To investigate the ability of recombinant N (rN) from different hantaviruses to elicit cross-protection, we immunized bank voles with rN from Puumala (PUUV), Topografov (TOPV), Andes (ANDV), and Dobrava (DOBV) viruses and subsequently challenged them with PUUV. All animals immunized with PUUV and TOPV rN were completely protected. In the group immunized with DOBV rN, 7 of 10 animals were protected, while only 3 of 8 animals were protected in the group immunized with ANDV rN, which is more closely related to PUUV rN than DOBV rN. Humoral and cellular immune responses after rN immunization were also investigated. The highest cross-reactive humoral responses against PUUV antigen were detected in sera from ANDV rN-immunized animals, followed by those from TOPV rN-immunized animals, and only very low antibody cross-reactivity was observed in sera from DOBV rN-immunized animals. In proliferation assays, T lymphocytes from animals immunized with all heterologous rNs were as efficiently recalled in vitro by PUUV rN as were T lymphocytes from animals immunized with homologous protein. In summary, this study has shown that hantavirus N can elicit cross-protective immune responses against PUUV, and the results suggest a more important role for the cellular arm of the immune response than for the humoral arm in cross-protection elicited by rN.

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“…The most recent bank vole range changes are due to the recolonization of Europe and especially Fennoscandia after the last ice age 10,000 years ago (29). Earlier it has been shown that these migrations have had an impact on the evolution of PUUV (24,38).…”

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“…1): Finland (45,46,48,62), Sweden (24,25,38), Norway (38), Denmark (3), Russia (3,16,48,49,66), Belgium (6,13), Austria (1,5), and Germany (19,43). So far, over 100 partial or complete PUUV sequences have been deposited in GenBank.…”

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confidence: 99%

“…Nevertheless, if the master genotype and phenotype have high fitness to the environment, the quasispecies populations might be stable for a long period of time. This is most probably the case for PUUV and other hantaviruses, which have been well adapted to their natural hosts since long ago.Previous publications on PUUV evolution (1,3,13,19,24,43,45,46,48,49) have focused mainly on specific geographical areas, including only a limited number of sequences, and all the phylogenetic analyses have been performed with distant matrix methods. Recently, a substantial set of new complete PUUV S segment sequences became available (references 3 and 13 and our unpublished results).…”

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Molecular Evolution of Puumala Hantavirus

Sironen

Vaheri

Plyusnin

2001

J Virol

114

112

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Puumala virus (PUUV) is a negative-stranded RNA virus in the genus Hantavirus, family Bunyaviridae. In this study, detailed phylogenetic analysis was performed on 42 complete S segment sequences of PUUV originated from several European countries, Russia, and Japan, the largest set available thus far for hantaviruses. The results show that PUUV sequences form seven distinct and well-supported genetic lineages; within these lineages, geographical clustering of genetic variants is observed. The overall phylogeny of PUUV is star-like, suggesting an early split of genetic lineages. The individual PUUV lineages appear to be independent, with the only exception to this being the Finnish and the Russian lineages that are closely connected to each other. Two strains of PUUV-like virus from Japan form the most ancestral lineage diverging from PUUV. Recombination points within the S segment were searched for and evidence for intralineage recombination events was seen in the Finnish, Russian, Danish, and Belgian lineages of PUUV. Molecular clock analysis showed that PUUV is a stable virus, evolving slowly at a rate of 0.7 ؋ 10 ؊7 to 2.2 ؋ 10 ؊6 nt substitutions per site per year.Puumala virus (PUUV) belongs to the genus Hantavirus of the family Bunyaviridae (12). Like other members of this family, PUUV is an enveloped virus with a segmented, singlestranded RNA genome of negative polarity. The large (L) segment of 6.5 kb encodes the viral RNA polymerase, the 3.7-kb medium (M) segment encodes the two surface glycoproteins, and the 1.8-kb small (S) segment encodes the nucleocapsid protein (N).The natural host of PUUV is the bank vole, Clethrionomys glareolus, which belongs to the Arvicolinae subfamily of the Muridae family. The bank vole is found in most of Europe, excluding the Mediterranean coast and the northernmost areas ( Fig. 1) (40). The virus causes a life-long persistent and asymptomatic infection in rodents (47). In contrast, in humans PUUV is pathogenic, causing nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome (HFRS) (8).PUUV from the following countries has been genetically characterized ( Fig. 1): Finland (45,46,48,62), Sweden (24,25,38), Norway (38), Denmark (3), Russia (3,16,48,49,66), Belgium (6, 13), Austria (1, 5), and Germany (19, 43). So far, over 100 partial or complete PUUV sequences have been deposited in GenBank. These include 38 complete S segment sequences, 9 complete M segment sequences, and 2 complete L segment sequences. In general, the phylogenetic relationships between different hantaviruses and their rodent hosts mirror each other, supporting the idea of coevolution of the virus and its host (44). Furthermore, different strains of a given hantavirus type, including PUUV, show geographical clustering (48, 49) reflecting the sometime complicated history of host range movements. The most recent bank vole range changes are due to the recolonization of Europe and especially Fennoscandia after the last ice age 10,000 years ago (29). Earlier it has been shown that these migr...

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Distribution and genetic heterogeneity of Puumala virus in Sweden

Cited by 54 publications

References 36 publications

Epidemiological Study of Hantavirus Infection in the Samara Region of European Russia

Epidemiological Study of Hantavirus Infection in the Samara Region of European Russia

Cross-Protection against Challenge with Puumala Virus after Immunization with Nucleocapsid Proteins from Different Hantaviruses

Molecular Evolution of Puumala Hantavirus

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