Distribution and molecular characterization of Citrus leaf blotch virus from Actinidia in Shaanxi province, China

Liu, Huan; Song, Shuang; Wu, Wei; Mi, Weili; Shen, Chuan; Bai, Bixin; Wu, Yunfeng

doi:10.1007/s10658-019-01677-3

Cited by 17 publications

(6 citation statements)

References 24 publications

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“…This study increased the number of viruses in the family Betaflexiviridae that infect kiwifruit to six: AcVA, AcVB, ASbLV, ASGV, CLBV, and AcVC. Among them, AcVA, AcVB, ASGV, and CLBV were found with high infection frequencies (14%–86%) in kiwifruit‐growing regions in the Shaanxi province of China (Liu et al , 2019; Zhao et al , 2019).…”

Section: Discussionmentioning

confidence: 99%

Occurrence and molecular characterization of Actinidia virus C (AcVC), a novel vitivirus infecting kiwifruit (Actinidia spp.) in China

et al. 2020

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Kiwifruit (Actinidia spp.) is an economically important fruit crop in the world. China is the largest producer of kiwifruit in terms of both acreage and yield. In this study, a novel virus, tentatively named Actinidia virus C (AcVC), was discovered in Actinidia deliciosa ‘Xuxiang’ grown in commercial kiwifruit fields in the Shaanxi province, a major kiwifruit‐growing region in China. Sequencing of the complete genome showed that AcVC has 7,668 nucleotides (not including the poly‐(A) sequence). Complete genome comparison and phylogenetic analysis showed that AcVC is most closely related to AcVA and then to AcVB. AcVC shares 69.2% nucleotide identity with AcVA (GenBank accession number ). Based on these data, Actinidia virus C is assigned as a new tentative member of the genus Vitivirus in the family Betaflexiviridae. AcVC was found to infect A. deliciosa and A. chinesis, the two major cultivated kiwifruits. Infection frequency of AcVC varied with the kiwifruit‐growing regions and cultivars in the Shaanxi province of China.

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Section: Discussionmentioning

confidence: 99%

Occurrence and molecular characterization of Actinidia virus C (AcVC), a novel vitivirus infecting kiwifruit (Actinidia spp.) in China

et al. 2020

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“…In China, CLBV has been found not only in lemon (Li et al 2018) but also in sweet cherry ) and kiwifruit (Liu et al 2019). Surprisingly, the average CLBV incidence rate was as high as 28.53% in kiwifruit grown in Shaanxi province (Liu et al 2019).…”

Section: Citrus Leaf Blotch Virusmentioning

confidence: 99%

Citrus Viroids and Minor Citrus Viruses in Japan

Iwanami

2023

JARQ

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Citrus species grown in Japan are affected by several viruses and viroids, including satsuma dwarf virus, citrus vein enation virus, citrus exocortis viroid, and other citrus viroids. Recently, a comprehensive review was made on the occurrence, history, and research of the following three major citrus viruses in Japan: satsuma dwarf virus, citrus tatter leaf virus (apple stem grooving virus), and citrus tristeza virus (Iwanami 2022). As a sequel, this study reviews the occurrence, history, and research development of citrus viroids as well as minor citrus viruses in and around Japan.

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“…A total of 9 kinds of viruses, including ASGV, CMV, AVX, CNV, RMV, CLBV, AcVB, PZSV, and CLRV, were detected via the RT-qPCR method [18,19].…”

Section: Rna Extraction and Rt-qpcr Detectionmentioning

confidence: 99%

“…However, in the process of in vitro rapid propagation of kiwifruit, serious browning and diseases of explants in primary culture have become a major obstacle [10,11]. In recent years, some literature has reported that many viruses, including apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), Actinidia virus B (AcVB), Actinidia virus A (AcVA), Actinidia virus 1 (AcV-1), alfalfa mosaic virus (AMV), cherry leaf roll virus (CLRV), tomato necrotic spot associated virus (TNSaV), tomato zonate spot virus (TZSV), Pelargonium zonate spot virus (PZSV), citrus leaf blotch virus (CLBV), Actinidia chlorotic ringspot-associated virus (AcCRaV), turnip vein clearing virus (TVCV), and Actinidia seed-borne latent virus (ASbLV), can infect kiwifruit plants through pollen, grafting, or artificial inoculation [12][13][14][15][16][17][18][19]. Once infected, viruses cause a detrimental effect on the growth and development of kiwifruit plants, and the fruits will be deformed and the yields will be reduced, with huge economic loss.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”

Zhong

Zhou

Tang

et al. 2021

Journal of Chemistry

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In order to breed virus-free plantlets of the kiwifruit cultivar “Guichang,” which belongs to Actinidia deliciosa, in this study, stem segments with buds were used as explants, the establishment of a tissue culture rapid propagation system was carried out, and then the virus status of tissue culture plantlets was detected via the real-time reverse transcription-polymerase chain reaction (RT-qPCR) method. The tissue culture rapid propagation system proved that the contamination and browning rates could be controlled below 20% and the survival rate could be exceeded by 70% when the single bud stem segment of “Guichang” kiwifruit was sterilized with 70% alcohol for 30–60 s and 15% NaClO for 15 min, respectively. Meanwhile, we screened the hormone concentration to get better results, and the appropriate medium for adventitious bud induction was MS + 6-BA (1.0 mg/L) + IBA (0.2 mg/L); for proliferation, it was MS + 6-BA (1.0 mg/L) + IBA (0.1 mg/L); and for rooting, it was 1/2 MS + IBA (0.3 mg/L), and the efficiency of induction, proliferation, and rooting could reach 74.07%, 79.63%, and 85.18%, respectively. In addition, the RT-qPCR results demonstrated that the infection rate of 9 viruses: apple stem grooving virus (ASGV), cucumber mosaic virus (CMV), Actinidia virus X (AVX), cucumber necrosis virus (CNV), ribgrass mosaic virus (RMV), citrus leaf blotch virus (CLBV), Actinidia virus B (AcVB), Pelargonium zonate spot virus (PZSV), and cherry leaf roll virus (CLRV) in the “Guichang” kiwifruit tissue culture plantlets was 0. This study could lay a foundation for the production of “Guichang” kiwifruit tissue culture seedlings, and the medium formula provided in this study was useful for the industrial rapid propagation of “Guichang” plantlets.

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Distribution and molecular characterization of Citrus leaf blotch virus from Actinidia in Shaanxi province, China

Cited by 17 publications

References 24 publications

Occurrence and molecular characterization of Actinidia virus C (AcVC), a novel vitivirus infecting kiwifruit (Actinidia spp.) in China

Occurrence and molecular characterization of Actinidia virus C (AcVC), a novel vitivirus infecting kiwifruit (Actinidia spp.) in China

Citrus Viroids and Minor Citrus Viruses in Japan

Establishment of Tissue Culture System of Actinidia deliciosa Cultivar “Guichang”

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