Distribution and ontogeny of thyrotropin-releasing hormone degrading enzymes in rats

Abstract

The distribution and ontogeny of tissue prolyl endopeptidase and pyroglutamyl peptidase I activities were studied in the rat from the 7th day before birth to adulthood. While low levels of prolyl endopeptidase activity were demonstrable in many fetal tissues, activity in brain cortex, hypothalamus, lung, and kidney increased dramatically during the 2 wk after birth, gradually returning to adult levels. In adult rats, levels of tissue prolyl endopeptidase activity were highest in kidney, when compared with the intermediate levels in brain cortex, hypothalamus, and liver. Pyroglutamyl peptidase activity was widely distributed in adult rat tissues with high levels in kidney and liver that exceeded intermediate levels in brain cortex and hypothalamus. Pyroglutamyl peptidase activities in fetal gut, brain, and lung tissue were elevated above adult values. In contrast to the development changes in prolyl endopeptidase activities, pyroglutamyl peptidase activity remained elevated above adult levels only during the first week of life. These results indicate that both prolyl endopeptidase and pyroglutamyl peptidase activities in the rat are developmentally regulated.

Effects of environmental enrichment on gene expression in the brain

“…Caspase-6 is involved in apoptosis by the degradation of poly(ADP-ribose) polymerase and DNA topoisomerase I (15). Recently, it has been shown that caspase-6 and prolyloligopeptidase participate in the processing of amyloid precursor protein (13,16,17). In addition, inhibitors of prolyloligopeptidase have been reported to enhance cognition and are being explored for the treatment of Alzheimer's disease (18,19).…”

Localization and subcellular distribution of prolyl oligopeptidase in the mouse placenta

“…1). This appears to be inconsistent with several studies reporting that the brain contained higher POP activity than other tissues (Fuse et al, 1990;Hersh 1981;Irazusta et al, 2002). However, the studies did not include the ovary and placenta for the assay and one report indicated that the ovary contained higher POP activity than the brain in the mouse (Ohta et al, 1992).…”

“…The POP enzymatic activity or its mRNA expression have been reported to be high at early developmental stages of the rat brain (Kato et al, 1980;Fuse et al, 1990;Yamanaka et al, 1999;Agirregoitia et al, 2003Agirregoitia et al, , 2010, pituitary (Agirregoitia et al, 2003), and liver (Matsubara et al, 1998) as well as of the mouse testis (Kimura et al, 2002).…”

MicroRNA-324-3p Promotes Renal Fibrosis and Is a Target of ACE Inhibition

“…35,36 Prep cleaves the peptide bond at the C-terminal side of an internal proline (-Pro-Xaa, where Xaa is any amino acid other than proline) of short 30-mer peptides. Peptides larger than this size do not gain access to the active site of the enzyme and are spared from proteolysis.…”