Distribution and organization of odontoblast processes in human dentin.

Gunji, Takahide; Kobayashi, Shígeo

doi:10.1679/aohc.46.213

Cited by 44 publications

(16 citation statements)

References 8 publications

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“…Gunji and Kobayashi (1983) and Yamada et al (1983) observed processlike structures extending to the DEJ, similar to those seen in the present study, after removing the extracellular organic matrix of dentine by means of an HC1-collagenase digestion. However, in order to minimize the risk of artefactual damage that can occur to the specimens with time, our samples were prepared in 3 days as compared to the 50 days of demineralization undertaken by Yamada et al (1983).…”

Section: Discussionsupporting

confidence: 91%

“…Recent SEM data have suggested that the odontoblast process extends to the DEJ (Kelley et al, 1981;Maniatopoulos and Smith, 1983;Grossman and Austin, 1983;Gunji and Kobayashi, 1983;Yamada et al, 1983). Gunji and Kobayashi (1983) and Yamada et al (1983) observed processlike structures extending to the DEJ, similar to those seen in the present study, after removing the extracellular organic matrix of dentine by means of an HC1-collagenase digestion.…”

Section: Discussionmentioning

confidence: 99%

“…For the past decade, transmission electron microscopic (TEM) investigations of dentine have also consistently suggested that the odontob-last processes are limited to the innedpulpal third of the dentinal tubules in rat (Garant, 1972), cat (Holland, 1975(Holland, , 1976 and human @'rank and Voegel, 1980; Thomas, 1979, Thomas andPayne, 1983) dentine. However, several recent SEM studies support the classical view that the odontoblast process extends to the DEJ in rat (Crooks et al, 1983), monkey (Kelley et al, 1981), Grossman andAustin, 1983 andhuman (Maniatopoulous andSmith, 1983;Gunji and Kobayashi, 1983;Yamada et al, 1983; teeth. Further complicating the issue is a series of TEM studies on human coronal demineralized dentine that demonstrated a n electron-dense structure within the lumen of the dentinal tubules (Thomas, 1979).…”

mentioning

confidence: 83%

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A combined scanning electron microscopy and immunofluorescence study demonstrating that the odontoblast process extends to the dentinoenamel junction in human teeth

et al. 1984

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The extent of the odontoblast cell process has been the subject of controversy for many years. Using SEM we have examined the extent and morphology of the process on dentine surfaces of human teeth which were partially demineralized and collagenase digested. Third molars were extracted and split; the dentine surface was demineralized, digested by bacterial collagenase, fixed with glutaraldehyde, postfixed in osmium tetroxide, and prepared for SEM investigation. The SEM study revealed the presence of many processlike structures which extended from the odontoblast cell bodies up to the dentinoenamel junction (DEJ). These processes demonstrated lateral and terminal branching and some of them terminated in distended spheres. We have also applied an immunofluorescence technique at the light microscope level to these exposed dentinal surfaces to localize the intracellular microtubules. For this, a second series of third molars was processed in the same manner as for the SEM up to the fixation stage. Teeth were then fixed in periodate-lysine-paraformaldehyde, postfixed in -20 degrees C acetone, and then incubated with affinity-purified rabbit antitubulin antibodies, followed by fluorescein-conjugated goat antirabbit IgGs. Intratubular immunofluorescence labelling for tubulin was evident from the odontoblast cell bodies up to the DEJ. The presence of the tubulin-containing structures extending to the DEJ supports the hypothesis that the structures observed with the SEM are odontoblast processes and that the odontoblast processes do extend to the DEJ.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 83%

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A combined scanning electron microscopy and immunofluorescence study demonstrating that the odontoblast process extends to the dentinoenamel junction in human teeth

et al. 1984

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“…These results confirm earlier transmission and scanning EM work (Maniatopoulos and Smith, 1983;Lilja, 1979;Thomas, 1979;Holland, 1975Holland, ,1976Brhnsuom and Garberoglio, 1972;Garant, 1972;Frank, 1968). Other EM studies that found OPs in outer dentin had all used rapid freezing of teeth with a closed pulp chamber before fixation (Frank and Steuer, 1988;Sigal and Cernecky, 1988;La Fleche et al, 1985;Sigal et al, 1984b;Gunji and Kobayashi, 1983;Maniatopoulos and Smith, 1983). However, when we rapidly froze rat jaws in the present study, the OPs were almost completely gone, compared with long OPs in inner dentin for perfusion-fixed teeth from the same rats, and we were unable to find any evidence for OPs in outer dentin.…”

Section: Discussioncontrasting

confidence: 73%

“…Many transmission and scanning electron microscopic studies have found that the OPs extend only into inner dentin and do not reach outer dentin or the dentinenamel junction (Thomas, 1979(Thomas, ,1984Thomas and Carella, 1984;Lilja, 1979;Holland, 1976;Brhmtrom and Garberoglio, 1972;Garant, 1972;Frank, 1966Frank, ,1968. However, others have found cell processes in outer dentin when whole teeth are exuacted and quickly frozen in liquid nitrogen before fixation (Frank and Steuer, 1988;Sigal and Cemecky, 1988;La Fleche et al, 1985;Sigal et al, 1984b;Gunji and Kobayashi, 1983;Maniatopoulos and Smith, 1983). The quick freezing may have captured OPs in an extended phase of a proposed flexiblelretractable cell framework for dentin (La Fleche et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Odontoblast processes in dentin revealed by fluorescent Di-I.

Byers

A²

1995

J Histochem Cytochem.

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There has been controversy about the length and structure of the odontoblast process within dentin since the earliest histologic studies of teeth. Our objective was to use the fluorescent carbocyanine dye Di-I combined with a new gelatin embedment procedure and confocal microscopy to determine the structure and extent of odontoblast processes in developing and mature rat teeth, injured rat molars, reparative dentin, and adult monkey teeth. We found that odontoblast processes do not extend into outer dentin or to the dentin-enamel junction except during early stages of development. Those in innervated regions of crown are long and straight, whereas those in roots are extensively branched and shorter. Cavity injury to crown dentin caused odontoblast fragments to be aspirated into outer dentin. In reparative dentin the odontoblast pmceses were branched and similar to those in roots. We used photoconversion and electron microscopy to show that Di-I f i i the entire odontoblast after gelatin embedment, including the cytoplasm. This is a different type of carbocganine staining from any previously reported, and it also stains other cells in adjacent hard tissues such as bone and cementum. The Di-I-gelatin method is a new way to use carbocyanine dyes. It has enabled us to solve a long-standing controvezsy about the histology of teeth, and it should be useful for many other studies of cell structure. ( J Hisrochem Cyrochem 43159-168, 1995)

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Structure and organization of odontoblasts

Sasaki

Garant

1996

Anat. Rec.

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Distribution and organization of odontoblast processes in human dentin.

Cited by 44 publications

References 8 publications

A combined scanning electron microscopy and immunofluorescence study demonstrating that the odontoblast process extends to the dentinoenamel junction in human teeth

A combined scanning electron microscopy and immunofluorescence study demonstrating that the odontoblast process extends to the dentinoenamel junction in human teeth

Odontoblast processes in dentin revealed by fluorescent Di-I.

Structure and organization of odontoblasts

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