Distribution and Pathogenicity of Two Cutthroat Trout Virus (CTV) Genotypes in Canada

Long, Amy; Leblanc, F.; Arseneau, Jean-René; Gagné, Nellie; Einer-Jensen, Katja; Lovy, Jan; Polinski, Mark P.; Jones, Simon R. M.; Garver, Kyle A.

doi:10.3390/v13091730

Cited by 6 publications

(1 citation statement)

References 37 publications

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“…It has been reported previously that HEV and RHEV do not typically produce CPE during virus isolation. However, cutthroat trout virus in the genus Piscihepevirus was reported to induce a diffuse CPE and incomplete destruction of CHSE-214 monolayers [52]. Therefore, to ascertain whether the novel hepevirus identified here could induce CPE, we performed observations twice daily, and did not observe any distinct CPE after six blind passages.…”

Section: Resultsmentioning

confidence: 87%

Isolation and characterization of a novel rodent hepevirus in long-tailed dwarf hamsters (Cricetulus longicaudatus) in China

Xu,

Bie,

et al. 2024

Journal of General Virology

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Hepeviruses have been identified in a broad range of animal hosts, including mammals, birds, and fish. In this study, rodents (n=91) from seven different species and ten pikas (Ochotona curzoniae) were collected in Qinghai Province, China. Using transcriptomic sequencing and confirmatory molecular testing, hepeviruses were detected in 27 of 45 (60 %) long-tailed dwarf hamsters (Cricetulus longicaudatus) and were undetected in other rodents and pika. The complete genome sequences from 14 representative strains were subsequently obtained, and phylogenetic analyses suggested that they represent a novel species within the genus Rocahepevirus, which we tentatively designated as Cl-2018QH. The virus was successfully isolated in human hepatoma (Huh-7) and murine fibroblast (17 Cl-1) cell lines, though both exhibited limited replication as assayed by detection of negative-sense RNA intermediates. A129 immunodeficient mice were inoculated with Cl-2018QH and the virus was consistently detected in multiple organs, despite relatively low viral loads. In summary, this study has described a novel rodent hepevirus, which enhances our knowledge of the genetic diversity of rodent hepeviruses and highlights its potential for cross-species transmission.

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Section: Resultsmentioning

confidence: 87%

Isolation and characterization of a novel rodent hepevirus in long-tailed dwarf hamsters (Cricetulus longicaudatus) in China

Xu,

Bie,

et al. 2024

Journal of General Virology

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Comment on a perspective: Molecular detections of new agents in finfish—Interpreting biological significance for fish health management

Mordecai,

Di Cicco,

Deeg

et al. 2024

J Aqua Anim Hlth

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Establishment and characterization of novel spontaneously immortalized larval cell lines from sablefish Anoplopoma fimbria

Friesen,

Long,

Garver

2024

In Vitro Cell.Dev.Biol.-Animal

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Sablefish Anoplopomafimbria is a groundfish of the North Pacific Ocean typically found in sea floor habitat at depths to 2700 m. Prized as a food fish with exceptionally high market value, sablefish aquaculture has been sought to provide a sustainable source of this fish to meet market demands. While commercial culture has successfully produced market-sized fish in Pacific coastal environments, production has been hampered by disease and the overall lack of information on sablefish health and immunology. To begin to address these knowledge gaps, herein we describe the isolation and characterization of spontaneously immortalized sablefish larval cell lines (AFL). Six sublines were established from pools of early yolk-sac larvae, while attempts to develop tissue-specific–derived cell lines were unsuccessful. The six yolk-sac larval cell lines each display two morphologies in culture, an elongated fibroblast-like cell type, and a rounded squamous or epithelial-like cell type. Cytogenetic characterization suggests that both cell types are diploid (2n = 48) with 24 pairs of chromosomes, 23 pairs of autosomes, and 1 pair of sex chromosomes. A small proportion (11%) of AFL cells display tetraploidy. Incubation temperature and medium composition experiments revealed HEPES buffered L-15 media containing 10–20% FBS at temperatures between 15 and 18° C yielded optimal cell growth. These growth characteristics suggest that sablefish larval cells display a robustness for varying growth conditions. The establishment of AFL cell lines provides a foundational tool to study the physiology, health, immunology, and cell and molecular biology of sablefish.

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Distribution and Pathogenicity of Two Cutthroat Trout Virus (CTV) Genotypes in Canada

Cited by 6 publications

References 37 publications

Isolation and characterization of a novel rodent hepevirus in long-tailed dwarf hamsters (Cricetulus longicaudatus) in China

Isolation and characterization of a novel rodent hepevirus in long-tailed dwarf hamsters (Cricetulus longicaudatus) in China

Comment on a perspective: Molecular detections of new agents in finfish—Interpreting biological significance for fish health management

Establishment and characterization of novel spontaneously immortalized larval cell lines from sablefish Anoplopoma fimbria

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