Distribution and Strain Diversity of Immunoregulating Segmented Filamentous Bacteria in Human Intestinal Lavage Samples

Chen, Huahai; Wang, Ling Ling; Wang, Xing; Wang, Xin; Liu, Haifeng; Yin, Yeshi

doi:10.1007/s00248-019-01441-4

Cited by 12 publications

(11 citation statements)

References 48 publications

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“…The prevalence of SFB among different animal species seems to be ubiquitous. SFB have been detected in a broad range of mammals like monkeys, horses, cattle, pigs, dogs, mice, rats, and in birds [1][2][3][4][5]. Interestingly, previous attempts to colonize germ-free rats or germ-free mice with SFB obtained from unrelated hosts have failed, indicating that SFB are host specific [15].…”

Section: Resultsmentioning

confidence: 99%

“…Even though the SFB abundance increases after weaning, not all weaned piglets are colonized with SFB (Figs. 1,2). The rationale for differences in colonization between the individual animals is not known yet.…”

Section: Resultsmentioning

confidence: 99%

“…The gut microbiota of pigs is characterized by rapid development after birth with certain bacteria temporarily dominating the intestinal tract. Segmented filamentous bacteria (SFB) are Gram-positive, spore-forming bacteria which have been detected in a wide range of mammals, birds, humans, and also in insects [1,2]. In rodents such as mice and rats, SFB are found predominantly around weaning and persist only shortly afterward [3,4].…”

Section: Introductionmentioning

confidence: 99%

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A Preliminary Survey of the Distribution of Segmented Filamentous Bacteria in the Porcine Gastrointestinal Tract

et al. 2021

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Segmented filamentous bacteria (SFB) are present in various animal species including pigs. The aim of this work was to analyze the occurrence of SFB in different parts of the gastrointestinal tract of piglets of different ages. A total of 377 DNA extracts from stomach, jejunum, ileum, cecum and colon digesta, and from feces collected on different time points, originating from 155 animals, were screened by qPCR method with primers specific for the SFB. SFB sequences were detected in 74 of 377 samples (19.6%) from 155 animals in total. SFB were most abundant in ileum (50.0%), cecum (45.0%), and colon (37.0%), followed by feces (14.6%). SFB prevalence in sows was 12.9% (13/101) and 75.9% (41/54) in individual piglets. Of the 41 SFB-positive piglets, only two samples were from pre-weaning animals, while the rest of samples originated from post-weaning piglets. SFB sequences are abundant in post-weaning piglets, but not in suckling or adult animals. They are most abundant in the ileum and cecum of pigs. Further studies are warranted to reveal the role of SFB in pigs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Preliminary Survey of the Distribution of Segmented Filamentous Bacteria in the Porcine Gastrointestinal Tract

et al. 2021

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“…More recently, 16S rRNA gene sequences of SFB were reported in human samples using SFB-specific PCR primers; Yin et al 28 found SFB sequences in 55 fecal samples while Jonsson 29 detected an SFB sequence in an ileostomy sample. The 16S sequences reported by Yin et al 28 were phylogenetically interleaved with SFB sequences from mice and additional data from Chen et al 30,31 also indicate that SFB with close relatedness to mouse SFB is present in humans. However, human gut shotgun metagenomic sample sets have been scanned by attempting to map reads to the SFB genomes of mice and rats, but without success 23 .…”

mentioning

confidence: 87%

Genome sequence of segmented filamentous bacteria present in the human intestine

et al. 2020

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Segmented filamentous bacteria (SFB) are unique immune modulatory bacteria colonizing the small intestine of a variety of animals in a host-specific manner. SFB exhibit filamentous growth and attach to the host's intestinal epithelium, offering a physical route of interaction. SFB affect functions of the host immune system, among them IgA production and T-cell maturation. Until now, no human-specific SFB genome has been reported. Here, we report the metagenomic reconstruction of an SFB genome from a human ileostomy sample. Phylogenomic analysis clusters the genome with SFB genomes from mouse, rat and turkey, but the genome is genetically distinct, displaying 65-71% average amino acid identity to the others. By screening human faecal metagenomic datasets, we identified individuals carrying sequences identical to the new SFB genome. We thus conclude that a unique SFB variant exists in humans and foresee a renewed interest in the elucidation of SFB functionality in this environment.

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“…Segmented filamentous bacteria (SFB) are ileum- and cecum-colonizing spore-forming gram-positive commensal bacteria 18,19 that have been detected in humans 20,21 and other vertebrates, as well as in invertebrates 22,23 . SFB are immunogenic and can induce the development of Th17 cells specific for SFB-derived antigens in the murine intestines 24, 25, 26 .…”

Section: Mainmentioning

confidence: 99%

Commensal-specific CD4 T cells promote inflammation in the central nervous system via molecular mimicry

White

Cabrera

Ochoa-Raya

et al. 2022

Preprint

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Commensal bacteria are critical regulators of both tissue homeostasis and the development and exacerbation of autoimmunity. However, it remains unclear how the intestinal microbiota contributes to inflammation in tissues such as the central nervous system (CNS) where these microbes are typically absent and whether T cell receptor (TCR) specificity for commensal-derived antigens is important to the development of tissue inflammation-related outcomes. Here, we found that ileum- and cecum-colonizing segmented filamentous bacteria (SFB)-specific T cells (clone TCR7B8) can infiltrate the CNS wherein they can be reactivated and produce high levels of inflammatory cytokines including IFNg, IL-17A, TNFa, and GM-CSF in the absence of regulatory T cells. In contrast, other SFB-specific T cells (clone TCR1A2) recognizing an epitope in which 8/9 amino acids overlap with those recognized by TCR7B8 failed to induce such neuroinflammation. Despite their similar SFB-derived peptide antigen targets, TCR7B8 was found to recognize peptides derived from host proteins including receptor tyrosine-protein kinase ErbB2, trophinin 1, and anaphase-promoting complex subunit 2 in vitro, whereas TCR1A2 did not, indicating that TCR7B8 induces CNS inflammation via molecular mimicry. Immune checkpoint blockade accelerated TCR7B8-mediated CNS inflammation, suggesting a potential cause of immune-related adverse events induced in cancer patients undergoing such treatment. Together, our findings reveal a potential mechanism whereby gut commensal-specific T cells are dysregulated and contribute to extraintestinal inflammation.

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Distribution and Strain Diversity of Immunoregulating Segmented Filamentous Bacteria in Human Intestinal Lavage Samples

Cited by 12 publications

References 48 publications

A Preliminary Survey of the Distribution of Segmented Filamentous Bacteria in the Porcine Gastrointestinal Tract

A Preliminary Survey of the Distribution of Segmented Filamentous Bacteria in the Porcine Gastrointestinal Tract

Genome sequence of segmented filamentous bacteria present in the human intestine

Commensal-specific CD4 T cells promote inflammation in the central nervous system via molecular mimicry

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