Distribution characteristics of organic selenium in Se-enriched Lactobacillus (Lactobacillus paracasei)

Sun, Yuhan; Wang, Huanzi; Zhou, Lei; Chang, Mengjia; Yue, Tian‐Li; Yuan, Yahong; Shi, Yiheng

doi:10.1016/j.lwt.2022.113699

Cited by 14 publications

(3 citation statements)

References 30 publications

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“…The findings indicated that the LAB were able to grow normally at Se concentrations of 5 and 10 µg/mL, while their growth was inhibited at higher Se concentrations of 20-40 µg/mL. Similar patterns of Se tolerance have also been observed in studies of L. reuteri, L. animalis, and L. casei, with L. casei demonstrating higher Se tolerance compared to other LAB [24,26,27].…”

Section: Discussionsupporting

confidence: 69%

Screening, Characterization and Probiotic Properties of Selenium-Enriched Lactic Acid Bacteria

Zan,

Chen,

Zhang

et al. 2024

Fermentation

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Considerable progress has been achieved in the bioaccumulation and transformation of selenium (Se)-enriched lactic acid bacteria (LAB). However, research on the effects of Se on the structure and probiotic potential of LAB is relatively limited. In this study, six industrial LAB strains, including Lactobacillus plantarum 21,805, Lactobacillus paracasei 20,241, Lactobacillus fermentum 21,828, Lactobacillus casei 23,185, Lactobacillus acidophilus 6064, and Lactobacillus plantarum 6076, were cultured in De Man, Rogosa, and Sharpe (MRS) medium containing Se for three generations. High-Se LAB was screened based on bacterial biomass, viable bacterial count, and Se content. Their structural characteristics were analyzed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The results indicated that LAB growth was influenced by the Se environment, with high Se (20 μg/mL) inhibiting it. At a Se concentration of 10 μg/mL, LAB maintained integrity and exhibited a high Se enrichment ability, with a Se enrichment of 4.88 ± 0.39 mg/g. The intracellular Se existed in an amorphous or non-crystalline form. Furthermore, Se-enriched LAB exhibited enhanced probiotic properties, including tolerance to simulated gastrointestinal fluids, acid resistance, bile salt resistance, and hydrophobicity. Therefore, Se-enriched LAB are expected to develop new functional foods or dietary supplements for human and animal consumption.

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Section: Discussionsupporting

confidence: 69%

Screening, Characterization and Probiotic Properties of Selenium-Enriched Lactic Acid Bacteria

Zan,

Chen,

Zhang

et al. 2024

Fermentation

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“…In addition, when the concentration of Na 2 SeO 3 was 2 μg/mL, the color of the strains in the experimental group was slightly red compared with the control group and gradually became deep red as the concentration increased (data not shown), indicating that when Na 2 SeO 3 was greater than 2 μg/mL, it was mainly converted into red elemental selenium (Se 0 ) by P. acidilactici MRS-7; the presence of Se 0 gives the food a metallic taste and affects the flavor of fermentation products. 27 Therefore, according to the Se accumulation rate and strain color, 2 μg/mL was selected as the concentration of Se for the subsequent test. Studies have shown that microorganisms could reduce selenite and selenate to organic Se by assimilation reduction or to Se 0 by dissimilation reduction, and the choice of assimilation or dissimilation reduction is closely related to the Se concentration.…”

Section: Preparation Of Sepmentioning

confidence: 99%

Enrichment and Distribution of Selenium in Pediococcus acidilactici MRS-7: Impact on Its Biochemical Composition, Microstructure, and Gastrointestinal Survival

Chen

Wang

Yue³

et al. 2022

J. Agric. Food Chem.

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Lactic acid bacteria can convert selenium (Se) from inorganic to organic and elemental forms, but the distribution and existence form of organic Se in the bacteria are not clear after Se enrichment, and the effects of selenization on the growth and nutritional value of strains also need to be studied. In this study, Pediococcus acidilactici MRS-7 could absorb up to 67% of inorganic Se and convert most of it into organic Se; about 75% of organic Se was selenoprotein, 2.7% was Se-polysaccharide, and 4.6% was Se-nucleic acid. Additionally, Se-enriched treatment increased the levels of amino acids and essential elements in P. acidilactici MRS-7. Finally, after Se enrichment, Se nanoparticles (SeNPs) were found on the surface of P. acidilactici MRS-7, but they had no harmful effect on its morphology, and its survival during gastrointestinal digestion was not affected, indicating that SeP has potential probiotic value in the food industry.

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“…Se can be supplemented through many ways, generally using inorganic Se and organic Se. Compared to inorganic Se, organic Se has lower toxicity, high bioavailability and low environmental pollution [ 6 , 7 ]. To convert inorganic Se into organic Se, biotransformations including plant transformation, animal transformation and microbial transformation are mainly carried out at present [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Screening and Identification of Soil Selenium-Enriched Strains and Application in Auricularia auricula

Chen,

Liu,

Zeng

et al. 2024

Microorganisms

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Selenium (Se) is an essential trace element for human physiological metabolism. The application of organic Se as a source to cultivate Se-rich plants for micronutrient supplementation has been receiving increasing attention. In our study, a bacterial strain named H1 was isolated from the soil in Heilongjiang Province, China, and under optimal culture conditions, the unit Se content could reach 3000 μg·g−1 and its 16S ribosomal DNA sequence seemed to be a new molecular record of an Enterobacter species. After the domestication of Se tolerance and Se-rich experiments, H1 can be used as a Se source for cultivation of Se-rich Auricularia auricula. The results showed that soluble protein, soluble sugar, free amino acid and vitamin C contents in Auricularia auricula were notably increased by 28.7%, 21.8%, 32.5% and 39.2% under the treatment of Se concentration of 0.24 mg·kg−1, respectively. These findings enhance our understanding that H1 is more conducive to Se uptake and nutrient accumulation.

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Distribution characteristics of organic selenium in Se-enriched Lactobacillus (Lactobacillus paracasei)

Cited by 14 publications

References 30 publications

Screening, Characterization and Probiotic Properties of Selenium-Enriched Lactic Acid Bacteria

Screening, Characterization and Probiotic Properties of Selenium-Enriched Lactic Acid Bacteria

Enrichment and Distribution of Selenium in Pediococcus acidilactici MRS-7: Impact on Its Biochemical Composition, Microstructure, and Gastrointestinal Survival

Screening and Identification of Soil Selenium-Enriched Strains and Application in Auricularia auricula

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