Distribution, cloning and sequencing of GnRH, its receptor, and effects of gastric acid secretion of GnRH analogue in gastric parietal cells of rats

Chen, Lei; Sun, Xuegang; Zhao, Jing; Yang, Angang; Huang, Wen‐Chang

doi:10.1016/j.lfs.2004.10.005

Cited by 17 publications

(21 citation statements)

References 44 publications

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“…Antibodies raised against GnRH2 are not commercially available. The GnRH antibodies used by others were produced by the groups themselves, and may differ in their affinity for GnRH2 and/or GnRH1 [5,6,7,13]. The different results between our and others´ result may be explained by different affinity of antibodies to GnRH2.…”

Section: Discussioncontrasting

confidence: 55%

“…Recently, our research group described GnRH expression in human submucous and myenteric neurons in both small and large intestine [12]. In rat, presence of GnRH and GnRH-R have been suggested in several cell types of the gastrointestinal tract [5,7,13,14], whereas such expression could not be confirmed by our group [15]. Notably, LH-R was found 4 to be expressed in enteric neurons in rat stomach, small and large intestine [15].…”

Section: Introductionmentioning

confidence: 61%

“…In contrast, other groups have found GnRH and GnRH-R immunoreactivity in rat enteric neurons, smooth muscle cells, and on parietal cells in the epithelium [5,6,7,13]. Our antibodies were raised against synthetic GnRH1; possible cross-13 reactivity to GnRH2 has not been able to evaluate.…”

Section: Discussionmentioning

confidence: 84%

“…During the last years, several studies have described effects by these hormones also on the gastrointestinal tract. GnRH analogs have been shown to inhibit gastric secretion and gastrin release in rat and dog [4,5], to inhibit cell proliferation of gastric epithelium [6] and smooth muscle cells [7], and to induce apoptosis and inhibit cell proliferation in gastric cancer cells [3,8]. Both GnRH analogs and LH have been shown to affect gastrointestinal motility in rat and man; GnRH analogs by stimulation of gastrointestinal motility and LH by prolonging and fragmenting of the phase III migrating motor complex (MMC) [9,10,11].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression and distribution of GnRH, LH, and FSH and their receptors in gastrointestinal tract of man and rat

Sand

Bergvall

Ekblad

et al. 2013

Regulatory Peptides

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General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal

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Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression and distribution of GnRH, LH, and FSH and their receptors in gastrointestinal tract of man and rat

Sand

Bergvall

Ekblad

et al. 2013

Regulatory Peptides

View full text Add to dashboard Cite

show abstract

“…In rats, GnRH and GnRH receptor mRNA and/or peptide has been found in ganglion cells of the myenteric plexus (8), on gastric smooth muscle cells (9) and on parietal cells in the epithelium (20). The role of GnRH in the gut is not completely elucidated, but GnRH analogs have been shown to inhibit gastric secretion and gastrin release in rat and dog (21,22), to inhibit cell proliferation in gastric epithelium (23), to protect enteric rat neurons in culture when continuously stimulated (7), whereas shorter stimulation inhibits cell proliferation in gastric smooth muscle cells (9), and to induce apoptosis and inhibited cell proliferation in several cancer cells (24).…”

Section: Discussionmentioning

confidence: 99%

Depletion of enteric gonadotropin-releasing hormone is found in a few patients suffering from severe gastrointestinal dysmotility

Hammar

Ohlsson

Veress

et al. 2012

Scandinavian Journal of Gastroenterology

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Depletion of enteric gonadotropin-releasing hormone is found in a few patients suffering from severe gastrointestinal dysmotility.Hammar, Oskar; Ohlsson, Bodil; Veress, Bela; Alm, Ragnar; Nordin Fredrikson, Gunilla; Montgomery, Agneta . (2012). Depletion of enteric gonadotropin-releasing hormone is found in a few patients suffering from severe gastrointestinal dysmotility. Scandinavian Journal of Gastroenterology, 47(10), 1165Gastroenterology, 47(10), -1173Gastroenterology, 47(10), . https://doi.org/10.3109/00365521.2012 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. GnRH and dysmotility AbstractObjective: Many patients, especially women, suffer from severe gastrointestinal pain and dysmotility for several years without being diagnosed. Depletion of gonadotropin-releasing hormone (GnRH) in the enteric nervous system (ENS) has been described in some patients.The aim of this study was to examine the expression of GnRH in ENS and antibodies against GnRH in serum, in a dysmotility patient cohort of southern Sweden. Materials and Methods:All consecutive patients (n=35) referred for laparoscopic fullthickness biopsy because of symptoms or signs of severe dysmotility between 1998 and 2009, or patients with a severe dysmotility disorder having had a bowel resection within the timeframe, were considered for inclusion. In 22 cases, representative biopsy material containing ganglia was available, and these patients were included. Medical records were scrutinized.The expression of GnRH was determined by immunohistochemistry in bowel biopsies from these patients and in patients with carcinoma or diverticulosis without ENS histopathology.Antibodies against GnRH in serum were determined by ELISA in patients and controls.Results: Fourteen patients were diagnosed with enteric dysmotility and 8 with chronic intestinal pseudo-obstruction due to varying etiology. Immunostained biopsies showed expression of GnRH in the ENS. A reduced expression of GnRH-containing neurons was found in 5 patients, as well as antibodies against GnRH in serum. Three of these patients had a history of in vitro fertilization (IVF) using GnRH analogs. Conclusions:A subgroup of patients with severe dysmotility had a reduced expression of GnRH-containing neurons in the ENS and expressed antibodies against GnRH in serum.

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APGW/AKH Precursor from Rotifer Brachionus plicatilis and the DNA Loss Model Explain Evolutionary Trends of the Neuropeptide LWamide, APGWamide, RPCH, AKH, ACP, CRZ, and GnRH Families

Cadena-Caballero,

Munive-Argüelles,

Vera-Cala

et al. 2023

J Mol Evol

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In the year 2002, DNA loss model (DNA-LM) postulated that neuropeptide genes to emerged through codons loss via the repair of damaged DNA from ancestral gene namely Neuropeptide Precursor Predictive (NPP), which organization correspond two or more neuropeptides precursors evolutive related. The DNA-LM was elaborated according to amino acids homology among LWamide, APGWamide, red pigment-concentrating hormone (RPCH), adipokinetic hormones (AKHs) and in silico APGW/RPCH NPPAPGW/AKH NPP were proposed. With the above principle, it was proposed the evolution of corazonin (CRZ), gonadotropin-releasing hormone (GnRH), AKH, and AKH/CRZ (ACP), but any NPP never was considered. However, the evolutive relation via DNA-LM among these neuropeptides precursors not has been established yet. Therefore, the transcriptomes from crabs Callinectes toxotes and Callinectes arcuatus were used to characterized ACP and partial CRZ precursors, respectively. BLAST alignment with APGW/RPCH NPP and APGW/AKH NPP allow identified similar NPP in the rotifer Brachionus plicatilis and other invertebrates. Moreover, three bioinformatics algorithms and manual verification were used to purify 13,778 sequences, generating a database with 719 neuropeptide precursors. Phylogenetic trees with the DNA-LM parameters showed that some ACP, CRZ, AKH2 and two NPP share nodes with GnRH from vertebrates and some of this neuropeptide had nodes in invertebrates. Whereas the phylogenetic tree with standard parameters do not showed previous node pattern. Robinson-Foulds metric corroborates the differences among phylogenetic trees. Homology relationship showed four putative orthogroups; AKH4, CRZ, and protostomes GnRH had individual group. This is the first demonstration of NPP in species and would explain the evolution neuropeptide families by the DNA-LM.

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Distribution, cloning and sequencing of GnRH, its receptor, and effects of gastric acid secretion of GnRH analogue in gastric parietal cells of rats

Cited by 17 publications

References 44 publications

Expression and distribution of GnRH, LH, and FSH and their receptors in gastrointestinal tract of man and rat

Expression and distribution of GnRH, LH, and FSH and their receptors in gastrointestinal tract of man and rat

Depletion of enteric gonadotropin-releasing hormone is found in a few patients suffering from severe gastrointestinal dysmotility

APGW/AKH Precursor from Rotifer Brachionus plicatilis and the DNA Loss Model Explain Evolutionary Trends of the Neuropeptide LWamide, APGWamide, RPCH, AKH, ACP, CRZ, and GnRH Families

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