Distribution, Detection Protocols and Characterization of Pseudomonas Syringae Pv. Actinidiae From Kiwifruit in Galicia (Northwest Spain)

Abelleira, A.; Ares, Aitana; Aguín, O.; Mansilla, P.; Lopez, Marcelo

doi:10.17660/actahortic.2015.1095.5

Cited by 10 publications

(19 citation statements)

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“…() and Abelleira et al . (). The biochemical and physiological resemblance between the Psa strains isolated in Portugal, the Japanese and the Italian strains (CFBP 4909 T and CFBP 7286, respectively) were also confirmed through the BIOLOG system (Biolog, Inc ), showing similar profiles.…”

Section: Discussionmentioning

confidence: 97%

“…; Abelleira et al . ). An alteration on the sequence of tox‐argK gene is also possible and it can block the amplification with this set of primers.…”

Section: Discussionmentioning

confidence: 97%

“…Determination of the FA profile was performed according to Abelleira et al . (). The bacterial cultures were replicated twice in TSBA (Trypticase Soy Broth Agar) and incubated 48 h at 28°C.…”

Section: Methodsmentioning

confidence: 97%

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Characterization ofPseudomonas syringaepv.actinidiaebiovar 3 on kiwifruit in north-west Portugal

García

Moura

Abelleira³

et al. 2018

J Appl Microbiol

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Aims Bacterial kiwifruit canker disease, caused by Pseudomonas syringae pv. actinidiae (Psa) was detected in north‐west Portugal in 2010, and has since caused significant losses. The objectives of this work were to characterize the Portuguese population(s) of Psa and to define the actual prevalence of Psa biovars in the most productive kiwifruit region in Portugal. Methods and Results Isolates obtained from Actinidia deliciosa orchards were characterized by morphological, biochemical, physiological, fatty acids and molecular tests (PCR, BOX‐PCR, duplex‐PCR, multiplex‐PCR and RFLP), phaseolotoxin, housekeeping and effector genes and pathogenicity. Results established that only Psa biovar 3 is present in the north‐west of Portugal, despite phenotypic and genetic variability among the isolates. Conclusions This work provides new information on P. syringae pv. actinidiae (Psa) genetic profile in Portugal, indicating for the first time, that two genetically different subpopulations of Psa biovar 3 are present. Significance and Impact of the Study A new subpopulation of Psa biovar 3 was found for the first time in Portugal, contributing to increase knowledge about this population worldwide and to support further understanding of the impact of Psa.

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Section: Discussionmentioning

confidence: 97%

“…; Abelleira et al . ). An alteration on the sequence of tox‐argK gene is also possible and it can block the amplification with this set of primers.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Characterization ofPseudomonas syringaepv.actinidiaebiovar 3 on kiwifruit in north-west Portugal

García

Moura

Abelleira³

et al. 2018

J Appl Microbiol

Self Cite

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show abstract

“…Determination of the FA profile was performed according to Abelleira et al . (). For microbial identification, the Sherlock ® system v. 4.5 (MIDI, Inc. Newark, DE, EE.UU.)…”

Section: Methodsmentioning

confidence: 97%

“…In surveys carried out in Pontevedra, Galicia (NW Spain), the main kiwifruit producing province in Spain, Abelleira et al . (, ) found Psa causing bacterial canker in orchards of A. deliciosa ‘Hayward’ and ‘Summer’, A. chinensis ‘Jin Tao’, and A. chinensis pollinator ‘Belen’, and obtained isolates corresponding all to the Psa3 population.…”

Section: Introductionmentioning

confidence: 99%