Distribution, gene sequence and expression<i>in vivo</i>of the plasmid encoded fimbrial antigen of<i>Salmonella</i>serotype Enteritidis

Woodward, Martin J.; Allen‐Vercoe, Emma; Redstone, Jacqueline S.

doi:10.1017/s0950268800001084

Cited by 29 publications

(23 citation statements)

References 38 publications

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“…on May 12, 2018 by guest http://aem.asm.org/ ulent phenotype in mice; e.g., 8 of 11 animal clinical isolates of serovar Bovismorbificans did not have the capacity to cause murine systemic disease. Additionally, consistent with previous reports, the plasmid-associated pef gene was serovar specific, i.e., common to members of some serovars (e.g., Enteritidis and Choleraesuis [28]) but absent in other serovars (e.g., Dublin [77]). (Serovar Arizonae contains spvB in the chromosome but does not contain the virulence plasmid [or pef] [10,48].)…”

Section: Resultssupporting

confidence: 91%

Human Salmonella Clinical Isolates Distinct from Those of Animal Origin

Heithoff

Shimp

Lau

et al. 2008

Appl Environ Microbiol

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The global trend toward intensive livestock production has led to significant public health risks and industry-associated losses due to an increased incidence of disease and contamination of livestock-derived food products. A potential factor contributing to these health concerns is the prospect that selective pressure within a particular host may give rise to bacterial strain variants that exhibit enhanced fitness in the present host relative to that in the parental host from which the strain was derived. Here, we assessed 184 Salmonella enterica human and animal clinical isolates for their virulence capacities in mice and for the presence of the Salmonella virulence plasmid encoding the SpvB actin cytotoxin required for systemic survival and Pef fimbriae, implicated in adherence to the murine intestinal epithelium. All (21 of 21) serovar Typhimurium clinical isolates derived from animals were virulent in mice, whereas many (16 of 41) serovar Typhimurium isolates derived from human salmonellosis patients lacked this capacity. Additionally, many (10 of 29) serovar Typhimurium isolates derived from gastroenteritis patients did not possess the Salmonella virulence plasmid, in contrast to all animal and human bacteremia isolates tested. Lastly, among serovar Typhimurium isolates that harbored the Salmonella virulence plasmid, 6 of 31 derived from human salmonellosis patients were avirulent in mice, which is in contrast to the virulent phenotype exhibited by all the animal isolates examined. These studies suggest that Salmonella isolates derived from human salmonellosis patients are distinct from those of animal origin. The characterization of these bacterial strain variants may provide insight into their relative pathogenicities as well as into the development of treatment and prophylactic strategies for salmonellosis.Salmonella enterica is a significant food-borne pathogen of humans transmitted via the consumption of meat, animal products, and food products (e.g., fruits and vegetables) contaminated with animal waste (24,41,76). Clinical manifestations of human and animal salmonellosis range from self-limiting gastroenteritis to severe bacteremia and typhoid fever. More than 2,300 serovars of S. enterica have been identified and classified previously, typically by serotyping based on antigenic variation in the lipopolysaccharide (O-antigen) and phase 1 (H1) and phase 2 (H2) flagella (22,45,46). Although serotyping has been an epidemiologically useful resource for classifying isolates, it provides relatively limited information regarding bacterial diversity, evolutionary relatedness, and pathogenicity (11,14,18,30,57,61).There are six subspecies of S. enterica, and the vast majority of human and animal infections are caused by strains belonging to subspecies I. In spite of the close genetic relationship among serovars assigned to subspecies I, there are significant differences in virulence, host adaptation, and host specificity (70, 71), and accordingly, they have been categorized into three different groups: broad...

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Section: Resultssupporting

confidence: 91%

Human Salmonella Clinical Isolates Distinct from Those of Animal Origin

Heithoff

Shimp

Lau

et al. 2008

Appl Environ Microbiol

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“…In addition, Salmonella Enteritidis produces PEF fimbriae encoded by the plasmid-localized pef operon (Woodward et al, 1996). SEF21 are type 1 fimbriae, which are the most common and one of the best-characterized enterobacterial adhesive structures (Abraham, 1994;Clegg & Gerlach, 1987).…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of the FimH adhesin from Salmonella enterica serovar Enteritidis

et al. 2006

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Salmonella enterica serovar Enteritidis has emerged during the last 20 years as the major causative agent of food-borne gastroenteritis in humans and as the major infectious agent on poultry farms, replacing Salmonella enterica serovar Typhimurium as the dominant pathogenic serovar. Because adhesion to gut tissues and colonization of the alimentary tract, mediated in large part by the FimH adhesins located on type 1 fimbriae, is an important stage in the pathogenesis of both serovars, the binding properties of the FimH adhesins from these two enteropathogens were compared. Salmonella Enteritidis FimH protein and the Salmonella Typhimurium low-adhesive variant of this adhesin were expressed in Escherichia coli and the recombinant proteins were analysed for their ability to bind glycoproteins carrying different oligomannosidic structures and different types of eukaryotic cells. In static binding assays (ELISA and Western blotting) both FimH proteins bound equally well to all three tested glycoproteins (RNase B, horseradish peroxidase and mannan-BSA). In addition, no differences were found in the binding specificity of the FimH proteins and intact cells of Salmonella Enteritidis and Salmonella Typhimurium to human colon carcinoma or bladder cancer cells. The presence of the same amino acid residues at positions 61 (glycine) and 118 (phenylalanine) and the similar binding properties of these two adhesins suggest that the newly described FimH protein of Salmonella Enteritidis represents the low-adhesive variant found in Salmonella Typhimurium. To study the binding specificity of Salmonella Enteritidis FimH protein further, direct kinetic analysis using surface plasmon resonance was performed. With this method it was found that Salmonella Enteritidis FimH adhesin bound with the highest K d value to high-mannose type N-glycans carried by RNase B; about 100 times lower K d values were obtained in the interactions with mannan-BSA and horseradish peroxidase.

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“…The plasmidencoded ®mbriae (PEF) and long polar ®mbriae (LPF) of Typhimurium are considered to be involved in the adhesion of this strain to villus small intestinal cells and Peyer's patches, respectively [8,9]. Enteritidis expresses PEF during infection in chickens [10], although its role in the infection process remains undetermined [11]. It also has the genetic potential to express LPF, but elaboration of this structure on Enteritidis has yet to be con®rmed [12].…”

Section: Introductionmentioning

confidence: 99%

Adhesion of Salmonella enterica var Enteritidis strains lacking fimbriae and flagella to rat ileal explants cultured at the air interface or submerged in tissue culture medium

Robertson¹,

Grant²,

Allen‐Vercoe³

et al. 2000

Journal of Medical Microbiology

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Rat ileal air interface and submerged explant models were developed and used to compare the adhesion of Salmonella enterica var Enteritidis wild-type strains with that of their isogenic single and multiple deletion mutants. The modi®ed strains studied were defective for ®mbriae,¯agella, motility or chemotaxis and binding was assessed on tissues with and without an intact mucus layer. A multiple a®mbriate=a¯agellate (®m 2 =¯a 2 ) strain, a ®mbriate but a¯agellate (¯a 2 ) strain and a ®mbriate=¯agellate but non-motile (mot 2 ) strain bound signi®cantly less extensively to the explants than the corresponding wild-type strains. With the submerged explant model this difference was evident in tissues with or without a mucus layer, whereas in the air interface model it was observed only in tissues with an intact mucus layer. A smooth swimming chemotaxis-defective (che 2 ) strain and single or multiple a®mbriate strains bound to explants as well as their corresponding wild-type strain. This suggests that under the present experimental conditions ®mbriae were not essential for attachment of S. enterica var Enteritidis to rat ileal explants. However, the possession of active¯agella did appear to be an important factor in enabling salmonellae to penetrate the gastrointestinal mucus layer and attach speci®cally to epithelial cells.

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Distribution, gene sequence and expressionin vivoof the plasmid encoded fimbrial antigen ofSalmonellaserotype Enteritidis

Cited by 29 publications

References 38 publications

Human Salmonella Clinical Isolates Distinct from Those of Animal Origin

Human Salmonella Clinical Isolates Distinct from Those of Animal Origin

Functional characterization of the FimH adhesin from Salmonella enterica serovar Enteritidis

Adhesion of Salmonella enterica var Enteritidis strains lacking fimbriae and flagella to rat ileal explants cultured at the air interface or submerged in tissue culture medium

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