In seasonally frozen regions, highway tunnels are prone to shallow buried bias pressures near the inlet/outlet, which leads to highway tunnels not only bearing asymmetric loads, but also facing the threat of extreme weather. However, there is still no clear understanding of the temperature field for topographically biased tunnel in seasonally frozen regions at present. Taking the Huitougou tunnel of Hegang-Dalian expressway as the object, this paper uses on-site monitoring, theoretical analysis, and numerical simulation to study the distribution law and influence factors of temperature field for topographically biased tunnel in seasonally frozen regions. The numerical results of the temperature field are in good agreement with the on-site monitoring data, which verified the accuracy of this numerical model based on the aerodynamic principle, turbulence model, and wall function method. Meanwhile, the effect of different slope angle and overburden thickness on the temperature field of the tunnel is further analyzed. It is found that when the slope angle increases, the temperature field in the tunnel surrounding rock changes accordingly. The connecting area between the surface and the tunnel temperature field is deflected from arch crown to the arch shoulder of the tunnel, resulting in a large change in the temperature of the shallow buried side, while minor change in the temperature of the deep buried side. The freezing depth of surrounding rock decreases with the rising slope angle. As the overburden thickness gradually increases, the temperature field of the surface surrounding rock and the tunnel surrounding rock gradually change from mutual influence to non-influence. When the overburden thickness exceeds 15 m, a “isolated temperature zone” appears in the middle with a temperature of 6~7 °C, the temperature field of the tunnel surrounding rock is basically not affected by the surface air temperature. These results can provide important theoretical and engineering guidance for the evaluation, construction, and maintenance of tunnel engineering in seasonally frozen regions.