Distribution of ?1A adrenergic receptor m RNA in the rat brain visualized by in situ hybridization

Domyancic, Anna V.; Morilak, David A.

doi:10.1002/(sici)1096-9861(19970929)386:3<358::aid-cne3>3.0.co;2-0

Cited by 115 publications

(89 citation statements)

References 47 publications

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“…The distribution of α 1 noradrenergic receptor staining was similar to the pattern of mRNA expression previously reported in the striatum and thalamus (Domyancic and Morilak, 1997). α 1 noradrenergic receptor staining was mostly cytoplasmic, and, in a few neurons, staining also was visible in the processes [ Figure 6B and E (drug-naive), C and F (LgA)].…”

Section: Resultssupporting

confidence: 83%

α1-Noradrenergic system role in increased motivation for cocaine intake in rats with prolonged access

Wee¹,

Mandyam²,

Lekic³

et al. 2008

European Neuropsychopharmacology

140

118

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In rodents, extended access to cocaine produces an escalation in cocaine self-administration that has face and construct validity for human compulsive drug intake. Here we report that rats with six-hour access (long access, LgA) to cocaine self-administration produced a higher breakpoint for cocaine using a progressive-ratio schedule than rats with one-hour access (short access, ShA), and prazosin (α 1 receptor antagonist) reduced the higher breakpoint for cocaine in LgA rats. Additionally, the number of neurons with α 1 -adrenergic receptor-like immunoreactivity in the bed nucleus of stria terminalis (BNST) was found to be much lower in LgA rats than in ShA and drug-naive rats. In contrast, UK14304 (α 2 receptor agonist) and betaxolol (β 1 receptor antagonist) had no effect on cocaine self-administration in either group. The data suggest that activation of the α 1 -noradrenergic system, perhaps in the BNST, is associated with increased motivation for cocaine in rats with extended access.

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Section: Resultssupporting

confidence: 83%

α1-Noradrenergic system role in increased motivation for cocaine intake in rats with prolonged access

Wee¹,

Mandyam²,

Lekic³

et al. 2008

European Neuropsychopharmacology

140

118

View full text Add to dashboard Cite

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“…The distribution of these receptors varies in different nuclei of the amygdala. The BLA expresses the a 1A adrenoceptor subtype almost exclusively (Day et al, 1997;Domyancic and Morilak, 1997). The role of these receptors in the amygdala's physiology and function has been unknown.…”

Section: Discussionmentioning

confidence: 99%

Stress Impairs α1A Adrenoceptor-Mediated Noradrenergic Facilitation of GABAergic Transmission in the Basolateral Amygdala

Braga

Aroniadou‐Anderjaska

Manion

et al. 2003

Neuropsychopharmacol

130

106

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Intense or chronic stress can produce pathophysiological alterations in the systems involved in the stress response. The amygdala is a key component of the brain's neuronal network that processes and assigns emotional value to life's experiences, consolidates the memory of emotionally significant events, and organizes the behavioral response to these events. Clinical evidence indicates that certain stressrelated affective disorders are associated with changes in the amygdala's excitability, implicating a possible dysfunction of the GABAergic system. An important modulator of the GABAergic synaptic transmission, and one that is also central to the stress response is norepinephrine (NE). In the present study, we examined the hypothesis that stress impairs the noradrenergic modulation of GABAergic transmission in the basolateral amygdala (BLA). In control rats, NE (10 mM) facilitated spontaneous, evoked, and miniature IPSCs in the presence of b and a 2 adrenoceptor antagonists. The effects of NE were not blocked by a 1D and a 1B adrenoceptor antagonists, and were mimicked by the a 1A agonist, A61603 (1 mM). In restrain/tail-shock stressed rats, NE or A61603 had no significant effects on GABAergic transmission. Thus, in the BLA, NE acting via presynaptic a 1A adrenoceptors facilitates GABAergic inhibition, and this effect is severely impaired by stress. This is the first direct evidence of stress-induced impairment in the modulation of GABAergic synaptic transmission. The present findings provide an insight into possible mechanisms underlying the antiepileptogenic effects of NE in temporal lobe epilepsy, the hyperexcitability and hyper-responsiveness of the amygdala in certain stress-related affective disorders, and the stress-induced exacerbation of seizure activity in epileptic patients.

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“…Presence of mRNA signal has previously been shown for a 1a , a 1b , a 2a , a 2c , b 1 , and b 2 adrenergic receptors in BST, and a 1a , a 1b , and a 2a adrenergic receptors in CeA (Nicholas et al, 1993a, b;Pieribone et al, 1994;Scheinin et al, 1994;Day et al, 1997;Domyancic and Morilak, 1997). We compared mRNA levels for these receptors in HR vs LR animals.…”

Section: Experiments Iv: Measurement Of Mrna Levels For Adrenergic Recmentioning

confidence: 94%

β1 Adrenergic Receptors in the Bed Nucleus of Stria Terminalis Mediate Differential Responses to Opiate Withdrawal

Cecchi

Capriles

Watson

et al. 2006

Neuropsychopharmacol

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The negative physical and affective aspects of opioid abstinence contribute to the prolongation of substance abuse. Withdrawal treatment is successful only in a subset of subjects, yet little is known about the neurobiological causes of these individual differences. Here, we compare the somatic and motivational components of opioid withdrawal in animals with high reactivity (HR) vs low reactivity (LR) to novelty, a phenotype associated with differential vulnerability to drug abuse. During withdrawal, HR relative to LR showed increased teeth chattering and eye twitching episodes, somatic signs associated with adrenergic modulation. Given the role of noradrenergic circuitry of the extended amygdala in opioid withdrawal, we examined adrenergic receptor gene expression in the bed nucleus of stria terminalis (BST) and central nucleus of the amygdala. Relative to LR, HR rats exhibit a selective increase in b 1 adrenergic receptor expression in lateral and medial BST. To uncover the functional relevance of this difference, we microinjected betaxolol, a selective b 1 receptor antagonist, into dorsal BST and assessed somatic and affective responses during withdrawal. Betaxolol microinjection dose-dependently decreased teeth chattering episodes in HR to levels observed in LR animals. Moreover, the antagonist blocked conditioned place aversion, a measure of negative affect associated with withdrawal, in HR but not in LR animals. Our results reveal for the first time that reactivity to novelty predicts somatic and affective aspects of opiate dependence, and that b 1 receptors in BST are implicated in opiate withdrawal but only in novelty-seeking individuals.

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Distribution of ?1A adrenergic receptor m RNA in the rat brain visualized by in situ hybridization

Cited by 115 publications

References 47 publications

α1-Noradrenergic system role in increased motivation for cocaine intake in rats with prolonged access

α1-Noradrenergic system role in increased motivation for cocaine intake in rats with prolonged access

Stress Impairs α1A Adrenoceptor-Mediated Noradrenergic Facilitation of GABAergic Transmission in the Basolateral Amygdala

β1 Adrenergic Receptors in the Bed Nucleus of Stria Terminalis Mediate Differential Responses to Opiate Withdrawal

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