Distribution of 2,3-Diaminohexuronic Acid in Strains of Propionibacterium and Other Bacteria

Abstract

Trichloroacetic acid extracts of 119 strains of bacteria were examined by thin-layer chromatography for the presence of 2,3-diamino-2,3-dideoxyhexuronic acid. This compound was identified by thin-layer chramatography as material giving a distinctive blue spot with a ninhydrin-collidine reagent and moving to the same position as authentic 2,3-diamino-2,3-dideoxyglucuronic acid. Material of this kind was found in all strains of proploriibacteria except Propionibacterium freudenreichii and also in some strains of Clostridium, Actinomyces, and Arachnia. It was not found in representatives of Corynebacterium (11 strains), Bacillus (4 strains), Lactobacillus (1 1 strains), Staphylococcus (3 strains), Nocardiu (3 strains), Streptomyces (1 strain), Actinoplanes (1 strain), Erysipelothrix (1 strain), Peptostreptococcus (2 strains), and Arcanobacterium (1 strain), but was present in one of the four strains of Eubacterium examined and in one of two strains of Bijidobucterium. It was not present in whole cells or lipopolysaccharides of seven strains of Bacteroides, but was found in two of five Pseudomonas aeruginosa strains tested.

A novel type of meso-diaminopimelic acid-based peptidoglycan and novel poly (erythritol phosphate) teichoic acids in cell walls of two coryneform isolates from the surface flora of French cooked cheeses

“…Oxidates were reduced by NaBH4 (10 mg/ml) in 0.1 M NH4OH. Separation and detection of diaminohexuronic acid was carried out by TLC according to Cummins (1985).…”

“…To clarify the nature of this compound, we considered the occurrence of a diaminodideoxyhexuronic acid, since such a sugar has been described as a constituent of cell wall polysaccharides (Cummins and White 1983) and lipopolysaccharides (Dmitriev et al 1982;Okuda and Suzuki 1983). Using the procedure described (Cummins 1985), the presence of a diaminodideoxyhexuronic acid in hydrolysates of CW-TRYP and in extracted teichoic acids was confirmed by the appearance of a characteristic bright blue spot on TL chromatograms. Further confirmation that this substance was a diaminodideoxyhexuronic acid was furnished by NMR-spectroscopy of isolated teichoic acid fragments (see below).…”