Distribution of 25-hydroxyvitamin D3 in dried blood spots and implications for its quantitation by tandem mass spectrometry

Kvaskoff, David; Ko, Pauline; Simila, Henry A.; Eyles, Darryl W.

doi:10.1016/j.jchromb.2012.05.040

Cited by 47 publications

(42 citation statements)

References 40 publications

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“…We previously observed that 25OHD concentrations in DBS varied with the position on the spot from which the 3.2-mm punch was made (15 ). In particular, we noticed increased concentrations in punches sampled from the periphery of the spot compared with centrally located punches.…”

Section: Discussionmentioning

confidence: 63%

“…We previously observed a bias toward increased 25OHD 3 concentrations in outer relative to inner locations when a 3.2-mm DBS was punched from the same 50-L spot (15 ). This corresponded with increased sample weight, and we believe this was related to issues of blood flow in heparinized whole blood.…”

Section: Spot Positionmentioning

confidence: 86%

“…Both patient and analytical samples were collected with heparin as an anticoagulant. It may be that our previous single analytical sample was not allowed to properly heparinize and some low level of clotting could have contributed to a greater distribution of the noncellular compartment of whole blood in peripheral regions, the socalled chromatographic effect previously discussed (15 ). Alternatively, perhaps the temperature or hydration status of the Whatman 903 blotting paper differed between the site of patient blood collection and our laboratory (19 ).…”

Section: Discussionmentioning

confidence: 98%

“…After using this assay for the assessment of vitamin D metabolites for the past 6 years, we wished to reassess interassay precision, but in a real-world context across studies of moderate to large patient cohorts (5,(7)(8)(9)(10)(11)13 ). A single in-house DBS sample with endogenous 25OHD 3 but no 25OHD 2 was prepared as previously described and stored at room temperature (3,15 ). This single sample was run in replicates of 6 within each patient assay (31 separate runs over the last 2 years) in the assessment of Ͼ12000 DBS samples.…”

Section: Assay Performance and Robustnessmentioning

confidence: 99%

“…As previously shown, 25OHD 3 recovery from both sera and DBS was not absolute. Recovery from DBS samples remained 3% lower than sera in samples vortex-mixed for the longest period [F (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) ϭ 2.66, P ϭ 0.032] (Fig. 5).…”

Section: Effect Of Vortex-mix Time On 25ohd 3 Recovery From Dbs Relatmentioning

confidence: 99%

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Minimizing Matrix Effects for the Accurate Quantification of 25-Hydroxyvitamin D Metabolites in Dried Blood Spots by LC-MS/MS

et al. 2016

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BACKGROUND The noncalcemic actions of vitamin D in multiple organs are now widely recognized. Vitamin D status has been linked with a wide variety of conditions, which has led to an increasing demand for vitamin D screening. In particular, there is intense interest in the impact of vitamin D on a variety of developmental conditions. The most readily accessible pediatric samples are dried blood spots, and health organizations are increasingly archiving such samples for later assessment of the antecedents of disease. METHODS In 2009, we developed a method to quantify the major circulatory form of vitamin D, 25-hydroxyvitamin D, in archived dried blood spots. Over the last 6 years, we have made substantial alterations to the published method to enhance throughput, sensitivity, and assay robustness. RESULTS With the alterations, the assay was 3 times faster than the previously published assay and had a >10-fold increase in signal strength. Intraassay imprecision decreased from 13.4% to 6.9%, and there was a 5-fold reduction in interfering phospholipids. In actual use over 2 years, the assay showed an interassay imprecision of 11.6%. CONCLUSIONS This assay has performed reliably over the past 6 years. The practical changes we have made should allow clinical chemists to successfully adapt this method.

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Section: Discussionmentioning

confidence: 63%

Section: Spot Positionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Section: Assay Performance and Robustnessmentioning

confidence: 99%

Section: Effect Of Vortex-mix Time On 25ohd 3 Recovery From Dbs Relatmentioning

confidence: 99%

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Minimizing Matrix Effects for the Accurate Quantification of 25-Hydroxyvitamin D Metabolites in Dried Blood Spots by LC-MS/MS

et al. 2016

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Neonatal vitamin D status and risk of multiple sclerosis

Ueda

Rafatnia

Bäärnhielm

et al. 2014

Annals of Neurology

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Dried blood spots: Concepts, present status, and future perspectives in bioanalysis

Sharma

Jaiswal

Shukla

et al. 2014

Drug Testing and Analysis

144

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Over the past several years, dried blood spot (DBS) sampling technique has emerged as a pertinent method in both qualitative and quantitative bioanalysis context. In the DBS method, the blood sample is directly soaked on to a paper (with or without treatment). After drying it can be analyzed by modern analytical, immunological, or genomic detection systems. Several advantages of the DBS technique such as low blood volume requirement, transportation and storage without special treatment, better analytes stability, enhanced clinical cooperation in clinical trials, and reduced unforeseeable exposure of analysts to biohazards, make it the most appropriate blood sampling technique. This review illustrates the information available on the DBS method which may serve as a single window for investigators in the field of bioanalysis. Also, it explores the proficiency and appliance of the DBS method in pharmacokinetic (PK), therapeutic drug monitoring (TDM), toxicokinetic (TK), metabolomic, and disease diagnosis.

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Distribution of 25-hydroxyvitamin D3 in dried blood spots and implications for its quantitation by tandem mass spectrometry

Cited by 47 publications

References 40 publications

Minimizing Matrix Effects for the Accurate Quantification of 25-Hydroxyvitamin D Metabolites in Dried Blood Spots by LC-MS/MS

Minimizing Matrix Effects for the Accurate Quantification of 25-Hydroxyvitamin D Metabolites in Dried Blood Spots by LC-MS/MS

Neonatal vitamin D status and risk of multiple sclerosis

Dried blood spots: Concepts, present status, and future perspectives in bioanalysis

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