Distribution of 28.5kDa antigen in the tegument of adult Fasciola gigantica

Anuracpreeda, Panat; Wanichanon, Chaitip; Chaithirayanon, Kulathida; Preyavichyapugdee, Narin; Sobhon, Prasert

doi:10.1016/j.actatropica.2006.09.006

Cited by 37 publications

(24 citation statements)

References 14 publications

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“…Interestingly, some of these proteins have been localized at the tegument of S. japonicum (44), and a recent proteomic study of the F. hepatica tegument revealed uncharacterized proteins with DM9 motifs to be associated with the tegumental membrane of the parasite (72), which supports our findings. On the other hand, numerous tegumental proteins lacking the DM9 motif have been identified and purified from F. gigantica (5)(6)(7)64), S. japonicum (69,70), S. mansoni (31,39,55), C. sinensis (18,74), Echinococcus granulosus (56), and Taenia spp. (40,42,54), and none of these proteins shares homology with FhTP16.5.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Characterization and Differential Expression of a 16.5-Kilodalton Tegument-Associated Antigen from the Liver Fluke Fasciola hepatica

Gaudier

Cabán-Hernández

Osuna

et al. 2012

Clin. Vaccine Immunol.

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ABSTRACTA cDNA encoding a 16.5-kDa protein termed FhTP16.5 was identified by immunoscreening of a cDNA library fromFasciola hepaticaadult flukes using pooled sera from rabbits infected withF. hepaticafor 4 weeks. Quantitative reverse transcriptase PCR (qPCR) analysis revealed that FhTP16.5 is not expressed in unembryonated eggs. It is poorly expressed in miracidia and highly expressed at the juvenile and adult stages; however, significant differences were found between the expression levels of FhTP16.5 in juveniles versus adult flukes. Recombinant FhTP16.5 was expressed at high levels inEscherichia coli, purified by affinity chromatography, and used to raise anti-FhTP16.5 polyclonal antibodies in rabbits. Immunoblot analysis using the anti-FhTP16.5 IgG antibody identified FhTP16.5 in crude and tegumental extracts and in excretory-secretory products ofF. hepatica. The protein was not detected in crude extracts ofSchistosoma mansoniorSchistosomajaponicum. Antibodies to FhTP16.5 were detected in the sera of rabbits at 3 to 12 weeks ofF. hepaticainfection as well as in the sera of humans with chronic fascioliasis; these findings suggest that FhTP16.5 could be a good antigen for serodiagnosis of fascioliasis. Immunohistochemistry demonstrated that FhTP16.5 localizes to the surface of the tegument of various developmental stages and in parenchymal tissues of the adult fluke. Such specific localization makes FhTP16.5 an attractive target for immunoprophylaxis or chemotherapy.

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Section: Discussionmentioning

confidence: 99%

Biochemical Characterization and Differential Expression of a 16.5-Kilodalton Tegument-Associated Antigen from the Liver Fluke Fasciola hepatica

Gaudier

Cabán-Hernández

Osuna

et al. 2012

Clin. Vaccine Immunol.

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“…Fasciola antigens are mostly released from the gut and the tegument into the blood circulation and excreted in stools of infected animals and humans (35)(36)(37)(38)(39). Several Fasciola antigens have been detected as circulating antigens in serum or as coproantigens in feces and were successfully used in immunodiagnosis of human fascioliasis (8,11,16,34,40).…”

Section: Discussionmentioning

confidence: 99%

Immunodetection of Fasciola gigantica Circulating Antigen in Sera of Infected Individuals for Laboratory Diagnosis of Human Fascioliasis

Attallah¹,

Bughdadi

El-Shazly

et al. 2013

Clin. Vaccine Immunol.

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c Currently, the laboratory diagnosis of human fascioliasis is based on the parasitological examination of parasite eggs in stool specimens and serological detection of specific antibodies in serum samples, which are often unreliable diagnostic approaches. Ideally, a sensitive and specific diagnostic test for Fasciola infection should be based on the detection of circulating Fasciola antigen, which implies active infection. Here, a 27-kDa-molecular-mass antigen was identified in a Fasciola gigantica adult worm antigen preparation, excretory-secretory products, and sera from F. gigantica-infected individuals, and it was not detected in antigenic extracts of other parasites and sera from noninfected individuals. The target antigen was isolated and partially characterized as a protein. Immunoperoxidase staining located the target epitope within teguments and guts of F. gigantica adult worms. The performance characteristics of a newly developed enzyme-linked immunosorbent assay (ELISA) based on F. gigantica circulating antigen detection in serum (FgCA-27 ELISA) were investigated using sera of 120 parasitologically diagnosed F. gigantica-infected individuals and 80 noninfected individuals. The area under the receiving operating characteristic (ROC) curve (AUC) for ELISA was significantly high (AUC ‫؍‬ 0.961, P < 0.0001) for discriminating Fasciola-infected and noninfected individuals. The developed assay showed high degrees of sensitivity, specificity, and efficiency (>93%), and a significant correlation (r ‫؍‬ 0.715, P < 0.0001) between antigen level and parasite egg count was shown. In conclusion, a 27-kDa Fasciola antigen was identified in sera of F. gigantica-infected individuals. A highly sensitive and specific Fasciola antigen detection assay, FgCA-27 ELISA, was developed for laboratory diagnosis of human fascioliasis.

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“…Other F. hepatica polypeptides that also play a role in host immunomodulation are the tegument antigens. The tegument constitutes the parasite-host interface and is the place where much of the immune interplay between the fluke and host occur (13–17). F. hepatica tegument antigens have been shown to significantly suppress the serum levels of gamma interferon (IFNγ) and interleukin-12p70 (IL12p70) and to suppress expression of the cell-surface markers CD80, CD86 and CD40 by targeting multiple TLRs of DCs (18).…”

Section: Introductionmentioning

confidence: 99%

Fasciola hepaticaFatty Acid Binding Protein Inhibits TLR4 Activation and Suppresses the Inflammatory Cytokines Induced by Lipopolysaccharide In Vitro and In Vivo

Martin

Cabán-Hernández

Figueroa-Santiago

et al. 2015

The Journal of Immunology

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Toll-like receptor 4 (TLR4), the innate immunity receptor for bacterial endotoxins, plays a pivotal role in the induction of inflammatory responses. There is a need to develop molecules that block either activation through TLR4 or the downstream signaling pathways to inhibit the storm of inflammation typically elicited by bacterial lipopolysaccharide (LPS), which is a major cause of the high mortality associated with bacterial sepsis. We report here that a single intraperitoneal injection of 15μg Fasciola hepatica fatty acid binding protein (Fh12) 1 hour before exposure to LPS suppressed significantly the expression of serum inflammatory cytokines in a model of septic shock using C57BL/6 mice. Because macrophages are good source of IL12p70 and TNFα, and critical in driving adaptive immunity, we investigated the effect of Fh12 on the function of mouse bone marrow derived macrophages (bmMΦs). Whereas Fh12 alone did not induce cytokine expression, it significantly suppressed the expression of IL12, TNFα, IL6 and IL1β cytokines as well as iNOS2 in bmMΦs, and also impaired the phagocytic capacity of bmMΦs. Fh12 had a limited effect on the expression of inflammatory cytokines induced in response to other TLR-ligands. One mechanism used by Fh12 to exert its anti-inflammatory effect is binding to the CD14 co-receptor. Moreover, it suppresses phosphorylation of ERK, p38 and JNK. The potent anti-inflammatory properties of Fh12 demonstrated here open doors to further studies directed at exploring the potential of this molecule as a new class of drug against septic shock or other inflammatory diseases.

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Distribution of 28.5kDa antigen in the tegument of adult Fasciola gigantica

Cited by 37 publications

References 14 publications

Biochemical Characterization and Differential Expression of a 16.5-Kilodalton Tegument-Associated Antigen from the Liver Fluke Fasciola hepatica

Biochemical Characterization and Differential Expression of a 16.5-Kilodalton Tegument-Associated Antigen from the Liver Fluke Fasciola hepatica

Immunodetection of Fasciola gigantica Circulating Antigen in Sera of Infected Individuals for Laboratory Diagnosis of Human Fascioliasis

Fasciola hepaticaFatty Acid Binding Protein Inhibits TLR4 Activation and Suppresses the Inflammatory Cytokines Induced by Lipopolysaccharide In Vitro and In Vivo

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