2008
DOI: 10.1002/cne.21754
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Distribution of a splice variant of choline acetyltransferase in the trigeminal ganglion and brainstem of the rat: Comparison with calcitonin gene‐related peptide and substance P

Abstract: Rat trigeminal ganglion neurons have been shown to contain a splice variant of choline acetyltransferase (pChAT). Here we report the distribution pattern of pChAT-containing afferents from the trigeminal ganglion to the brainstem, compared with that of calcitonin gene-related peptide (CGRP) and substance P (SP), by use of the immunohistochemical techniques in the rat. Most of CGRP(+) SP(+) ganglion cells contain pChAT, whereas half of the pChAT(+) ganglion cells possess neither CGRP nor SP. In the brainstem, p… Show more

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Cited by 30 publications
(38 citation statements)
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“…It specifically stains primary afferent neurons in rats, as shown by previous publications (Heglind et al, 2005, Yasuhara et al, 2008Krajnik et al, 2009Krajnik et al, , 2010Mousa et al, 2010). SP-specific immunolabeling with this antibody in rat brain was completely abolished by preabsorption with the synthetic SP peptide (Yasuhara et al, 2008).…”
Section: Characterization Of Antibodiessupporting
confidence: 53%
“…It specifically stains primary afferent neurons in rats, as shown by previous publications (Heglind et al, 2005, Yasuhara et al, 2008Krajnik et al, 2009Krajnik et al, , 2010Mousa et al, 2010). SP-specific immunolabeling with this antibody in rat brain was completely abolished by preabsorption with the synthetic SP peptide (Yasuhara et al, 2008).…”
Section: Characterization Of Antibodiessupporting
confidence: 53%
“…This hypothesis well correlates with the observation suggesting that CGRP is not only released at the end of the central axon terminal of the sensory neurons but also at the peripheral end, that is, at the receptor of the afferent fibers (Lundberg et al 1992). In CGRP immunoreactive cell bodies, SP immunoreactivity was also demonstrated (Lee et al 1985;Yasuhara et al 2008). The multiple coexistence is also a feature of terminal varicosities.…”
Section: Discussionsupporting
confidence: 77%
“…The question arises on what role neuropeptides has in the sensory neurons. The principal neurotransmitter of these neurons is an excitatory amino acid, the glutamate and an inhibitory amino acid, γ-aminobutyric acid (GABA) (Wanaka et al 1987;De Biasi and Rustioni 1988;Stoyanova et al 1998) and acetylcholin (Bellier and Kimura 2007;Yasuhara et al 2008). It is accepted by the The diameter of secretin-expressing cells is much greater than that of CGRP, SP and VIP immunoreactive cells.…”
Section: Discussionmentioning
confidence: 98%
“…0906) is a polyclonal antibody raised in goat against synthetic rat Tyr-CGRP (amino acid residues 23-37) and is thought to react with the whole CGRP molecule (amino acid residues 1-37) and the C-terminal fragment (amino acid residues 23-37; manufacturer's information). Western blot analysis has been used to verify the specificity of the anti-CGRP antibody in rat medulla extracts, and pre-absorption with the manufacturer's synthetic CGRP peptide abolishes immunostaining (Yasuhara et al 2008). The anti-GFAP (Advanced Immunochemicals; no.…”
Section: Antibody Characterization and Specificitymentioning
confidence: 99%