Introduction: India is a vast country with wide diversity in race, religion & creed. The same diversity has been observed in geographical distribution of blood groups in population within country. There are differences in the distributions of ABO, & Rh(D) blood groups amongst different populations. The study of blood grouping is important as it plays an important role in various genetic studies, in clinical studies for reliable geographical information and in blood transfusion practice as it will help a lot in reducing the morbidity and mortality rate. Methods: This study presents data about the prevalence of ABO & Rhesus (Rh) blood groups amongst blood donors during a period of one year (retrospective study from January 2014 to December 2014) conducted at Blood Bank of Shaheed Hasan Khan Mewati Govt. Medical College, Nalhar, Distt. Mewat, Haryana, India Results: During the study period, blood groups of 2724 donors were screened by antigen antibody agglutination method using commercially available antiseras which were validated at our Blood Bank. The study revealed that the commonest ABO blood group was B (38.83%) followed by O (28.70 %), A (22.90 %) and AB (9.54 %) respectively. Rh D Positive blood donors were 90.72 % and Rh D negative were 9.28 %.