Distribution of Aconitum Alkaloids in Body Fluids and Tissues in a Suicidal Case of Aconite Ingestion

Ito, Kitae; Tanaka, Satoshi; Funayama, Masato; Mizugaki, Michinao

doi:10.1093/jat/24.5.348

Cited by 56 publications

(34 citation statements)

References 15 publications

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“…by eating plant material or by the uptake of Aconitum preparations. The suicidal and homicidal use of aconite tubers has also been reported [12,13,14,15,16,17,18,19,20,21].…”

Section: Introductionmentioning

confidence: 95%

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Determination of aconitine in body fluids by LC-MS-MS

et al. 2004

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A very sensitive and specific method was developed for the determination of aconitine, the main toxic alkaloid from plants of the genus Aconitum L., in biological samples. The method comprised solid-phase extraction using mixed-mode Cs cation exchange columns followed by liquid chromatography-tandem mass spectrometry (LC-MS-MS). Chromatographic separation was achieved with a RP8 column. Detection of aconitine was achieved using electrospray in the positive ionisation mode and quantification was performed using multiple reaction monitoring with m/z 646.4 as precursor ion, i.e. [M+H]+ of aconitine and m/z 586.5, m/z 526.4 and m/z 368.4 as product ions after collision-induced dissociation. The method was fully validated for the analysis of blood samples: the limit of detection and the limit of quantitation were 0.1 ng/g and 0.5 ng/g, respectively. Within the linear calibration range of 0.5-25 ng/g, analytical recovery was 79.9%. In two fatal cases with suspected aconite intoxication, aconitine could be detected in blood samples at concentrations of 10.0 and 12.1 ng/g. In one case, aconitine could also be detected in the stomach content (3 ng/g) and in the other in the urine (180 ng/ml).

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“…by eating plant material or by the uptake of Aconitum preparations. The suicidal and homicidal use of aconite tubers has also been reported [12,13,14,15,16,17,18,19,20,21].…”

Section: Introductionmentioning

confidence: 95%

“…For the analysis of the Aconitum alkaloids in biological specimens such as blood, serum and urine, several GC-MS methods have been described [12,18,21,28,29]. These employ a variety of extraction procedures followed by derivatisation to their TMS derivatives.…”

Section: Introductionmentioning

confidence: 99%

Determination of aconitine in body fluids by LC-MS-MS

et al. 2004

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“…Some groups have described GC-MS and LC-MS methods for analysis of Aconitum alkaloids in body fluids, tissues, and human urine [12][13][14]. However, no report on the determination of Aconitum alkaloids based on CE-electrochemiluminescence (CE-ECL) appeared to date.…”

Section: Introductionmentioning

confidence: 99%

Facile separation and determination of Aconitine alkaloids in traditional Chinese medicines by CE with tris(2,2'‐bipyridyl) ruthenium(II)‐based electrochemiluminescence detection

Yin

Guo

et al. 2006

Electrophoresis

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A facile CE method coupled with tris(2,2'-bipyridyl) ruthenium(II)-based electrochemiluminescence [Ru(bpy)(3) (2+)] detection was developed for simultaneous determination of Aconitum alkaloids, i.e., hypaconitine (HA), aconitine (AC), and mesaconitine (MA) in baseline separation. The optimal separation of these Aconitum alkaloids was achieved in a fused-silica capillary column (50 cm x 25 microm id) with 30 mM phosphate solution (pH 8.40) as running buffer at 12 kV applied voltage. The three alkaloids can be determined within 10 min by a single run. The calibration curves showed a linear range from 2.0 x 10(-7) to 2.0 x 10(-5) M for HA, 3.4 x 10(-7) to 1.7 x 10(-5) M for AC, and 3.8 x 10(-7) to 1.9 x 10(-5) M for MA. The RSDs for all analytes were below 3.01%. Good linear relationships were found with correlation coefficients for all analytes exceeding 0.993. The detection limits were 2.0 x 10(-8) M for HA, 1.7 x 10(-7) M for AC, and 1.9 x 10(-7) M for MA under optimal conditions. This method was successfully applied to determine the three alkaloids in Aconitum plants.

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“…[6][7][8][9][10] The techniques used include high-performance liquid chromatography (HPLC) with UV detection, 6) HPLC with mass spectrometry (MS) 7,8) and gas chromatography (GC) with MS detection. 9,10) But the blood concentration of BLA was extremely low because of the low dose and rapid metabolism of the drug. Therefore, it is necessary to develope a highly sensitive and specific method to determine BLA in blood.…”

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confidence: 99%