Distribution of Adrenergic and Cholinergic Nerves in the Porcine Myometrium During the Oestrous Cycle. A Histochemical Investigation

Thilander, G.; Rodriguez‐Martinez, Heriberto

doi:10.1111/j.1439-0442.1989.tb00731.x

Cited by 7 publications

(8 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The contractile activity of the porcine myometrium is controlled by many factors including adrenergic, cholinergic and non-adrenergic non-cholinergic innervation (Łakomy 1987, Thilander et al 1989, Taneike et al 1991, Kaleczyc 1994, Majewski et al 1995, as well as endocrine, paracrine and autocrine factors (Kitazawa et al 1997, Cao et al 2002, Sukjumlong et al 2009, Franczak and Bogacki 2009. The most important substances regulating porcine uterine contractions include: noradrenaline (NA), serotonine (Kitazawa et al 2001a), acetylcholine (Ach) (Kitazawa et al 2003), oxytocin (Carnahan et al 1999, Kitazawa et al 2001b, prostanoids (prostaglandin F 2 α, E 1 and E 2 ) (Kucharski et al 2007, Jana et al 2010, tromboxane A 2 , histamine (Kitazawa et al 1997), neuropeptide Y (Markiewicz et al 2003) and endothelin (Isaka et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Markiewicz¹,

Kamińska²,

Bogacki³

et al. 2012

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

Recent studies show that a representative of phospholipids, namely lysophosphatidic acid (LPA) and its receptors (LPA 1-3 ) play a significant role in the reproductive processes, i. a, in the modulation of the uterine contractility. The participation of LPA 3 in the reproductive processes has been revealed in mice and has not been studied in gilts. Therefore, in the present study we investigated the role/action of LPA and its receptors LPA 1 , LPA 2 and LPA 3 on the contraction activity in the porcine uterus. The study was conducted on an experimental model in which the pig uterus consisted of the one whole uterine horn and a part of the second horn, both connected with the uterine corpus. Uterine strips consisting of the endometrium with the myometrium (ENDO/MYO) and myometrium (MYO) alone were collected on days 12-14 of the estrous cycle (control group; n = 5) or pregnancy (experimental group; n = 5). Two analogues of LPA at increasing doses were used: oleoyl-sn-glycero-3-phosphate (L-α-LPA, a selective agonist of LPA 1 and LPA 2 receptors; 10 -7 M; 10 -6 M and 10 -5 M) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT, a selective agonist of LPA 3 receptor; 68 nM; 136 nM and 680 nM). L-α-LPA caused an increase in the contraction tension, amplitude and frequency of ENDO/MYO from the uterine horn with the developing embryos. This effect was not observed in MYO in both groups examined. In the ENDO/MYO strips of the uterine horn with developing embryos, OMPT significantly increased the contraction tension at the highest dose (680 nM) and amplitude at all doses examined, while frequency of contractions was decreased at doses of 136 nM and 680 nM. In the MYO strips of the uterine horn with embryos a significant increase in the contraction tension and amplitude after the highest dose of OMPT was observed. The results obtained imply the important role of receptors LPA 1 , LPA 2 and LPA 3 in the contraction activity of the porcine uterus during early pregnancy.

show abstract

Section: Introductionmentioning

confidence: 99%

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Markiewicz¹,

Kamińska²,

Bogacki³

et al. 2012

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…Few investigations, however, of the innervation of the female reproductive tract in pigs have been carried out (21,22,33,46). Electron microscopic examinations of the pregnant and parturient porcine myometrium revealed a diminished amount of the scanty adrenergic nerves (47,48).…”

Section: Introductionmentioning

confidence: 99%

Adrenergic and Cholinergic Nerve Supply in the Porcine Myometrium and Cervix

Thilander

1989

Journal of Veterinary Medicine Series A

Self Cite

View full text Add to dashboard Cite

Summary The distribution of adrenergic and acetylcholinesterase‐containing (cholinergic) nerves in the porcine myometrium and cervix was investigated neurohistochemically. In early and mid‐pregnancy the number of adrenergic nerves increased from the adtubal part of the horns to corpus uteri, being more abundant in non‐placental than in placental regions. No obvious difference in the distribution of cholinergic nerves was observed among the regions examined. The cervix was richest innervated by both types of nerves throughout gestation. The diameter of the adrenergic nerves decreased during pregnancy, while cholinergic nerves showed no such a decrease. In late pregnancy and at parturition the adrenergic innervation was richest at the adtubal part of the horns and in the cervix and scanty in the rest of the uterus. Adrenergic nerves were seen both in vascular and in non‐vascular smooth muscle, while cholinergic nerves were mostly associated to blood vessels. Zusammenfassung Adrenerge und cholinerge Innervierung des Myometriums und der Cervix beim Schwein. Eine histochemische Untersuchung während der Trächtigkeit und der Geburt Die Verteilung von adrenergen und Azetylcholinesterase‐haltigen (cholinergen) Nerven im Myometrium und in der Cervix des Schweines wurde neurohistochemisch untersucht. Während der frühen und mittleren Trächtigkeit nahm die Anzahl der adrenergen Nerven vom oviduktnahen Bereich der Uterushörner zum Corpus uteri hin zu, wobei sie in Placenta‐freien Arealen häufiger waren als in Arealen mit Placenta. Bezüglich der Verteilung der cholinergen Nerven ergaben sich zwischen verschiedenen Uterusabschnitten keine auffälligen Unterschiede. Während der gesamten Trächtigkeit war die Cervix mit beiden Typen von Nerven am ausgeprägtesten innerviert. Der Durchmesser der adrenergen Nerven verringerte sich während der Trächtigkeit. Für die cholinergen Nerven trifft dies nicht zu. Während der späten Trächtigkeit und zum Zeitpunkt der Geburt war die adrenerge Innervation im oviduktnahen Bereich der Uterushörner und in der Cervix am ausgeprägtesten, während sie im restlichen Uterus spärlich war. Adrenerge Nerven wurden in der vaskulären und nicht‐vaskulären glatten Muskulatur beobachtet, während die cholinergen Nerven meist mit Blutgefäßen vergesellschaftet waren.

show abstract

“…, 1998; Kulkarni et al. , 1976; Thilander & Rodriguez‐Martinez, 1989; Thorbert, Alm, Omen & Sjoberg, 1977). Parasympathetic cholinergic fibers, via the pelvic nerve, non‐uniformly distribute in the myometrium and vasculature of the uterus in mice, rats, guinea pigs, and pigs (Moscarini, Cantagalli, Cavallotti, De Luca & Amenta, 1982; Papka et al.…”

Section: Introductionmentioning

confidence: 99%

“…Parasympathetic cholinergic fibers, via the pelvic nerve, non‐uniformly distribute in the myometrium and vasculature of the uterus in mice, rats, guinea pigs, and pigs (Moscarini, Cantagalli, Cavallotti, De Luca & Amenta, 1982; Papka et al. , 1999; Thilander & Rodriguez‐Martinez; Thorbert et al. ).…”

Section: Introductionmentioning

confidence: 99%

“…The uterus is one of the organs that receive abundant autonomic innervation. Sympathetic adrenergic fibers, via the hypogastric nerve, widely terminate in the smooth muscles and blood vessels of the upper and lower regions of the uterus and the cervix in rats, guinea pigs, pigs, and horses (Bae et al, 2001;Houdeau et al, 1998;Kulkarni et al, 1976;Thilander & Rodriguez-Martinez, 1989; Thorbert, Alm, Omen & Sjoberg, 1977). Parasympathetic cholinergic fibers, via the pelvic nerve, non-uniformly distribute in the myometrium and vasculature of the uterus in mice, rats, guinea pigs, and pigs (Moscarini, Cantagalli, Cavallotti, De Luca & Amenta, 1982;Papka et al, 1999; Thilander & Rodriguez-Martinez; Thorbert et al).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decreased cardiac parasympathetic nerve activity of pregnant women during foot baths

Miyazato

Matsukawa

2010

Japan Journal Nursing Sci

View full text Add to dashboard Cite

Aim: This study aimed to examine the effect of foot baths on the cardiac parasympathetic outflow of five pregnant (31-32 weeks; mid-pregnancy) women and 16 non-pregnant women. Methods: The cardiac parasympathetic outflow was assessed by using the respiratory variability of the R-R interval under controlled breathing according to three different methods: (i) respiratory sinus arrhythmia; (ii) a respiratory-synchronized component of the power spectrum of R-R interval variability with a fast Fourier transform; and (iii) a high-frequency component (HF at 0.15-0.40 Hz) of the power spectrum of R-R interval variability with a maximum entropy method. Results: The rate and amplitude of spontaneous respiration and arterial blood pressure at rest were the same between the non-pregnant and pregnant women. However, the baseline R-R interval was shorter and the respiratory-synchronized and HF components of the power spectrum of R-R interval variability were smaller in the pregnant women, indicating a decreased baseline cardiac parasympathetic outflow with midpregnancy. Although a foot bath for 15 min did not significantly affect the systemic hemodynamics and body temperature of both groups, the foot bath reduced the respiratory arrhythmia and the respiratorysynchronized and HF components of the power spectrum of R-R interval variability in both groups. The reduction in the respiratory R-R interval became more prominent in the pregnant group. Conclusion: A foot bath, used as part of midwives' daily nursing care, is able to decrease the cardiac parasympathetic outflow of pregnant women.

show abstract

Distribution of Adrenergic and Cholinergic Nerves in the Porcine Myometrium During the Oestrous Cycle. A Histochemical Investigation

Cited by 7 publications

References 25 publications

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Adrenergic and Cholinergic Nerve Supply in the Porcine Myometrium and Cervix

Decreased cardiac parasympathetic nerve activity of pregnant women during foot baths

Contact Info

Product

Resources

About