Distribution of aflatoxin M1 in cheese obtained from milk artificially contaminated

López, C.; Ramos, Laura; Ramadán, Silvana; Bulacio, Lucía; Pérez, Javier

doi:10.1016/s0168-1605(00)00444-x

Cited by 79 publications

(61 citation statements)

References 19 publications

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“…The mean concentrations of high incidence of AFM 1 in positive samples of Minas Frescal and Minas Padrao cheese were 0.142±0.118 and 0.118±0.054 ng g -1 , respectively. In another study, fresh cheese produced in Argentina from artificially contaminated milk with AFM 1 at levels of 1.7-2.0 ng mL -1 had 60% of AFM 1 in the whey and 40% in cheese [32] .…”

Section: Discussionmentioning

confidence: 95%

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To Determine the Occurence of Aflatoxin M1 (AFM1) in Samples of Cyprus Traditional Cheese (Halloumi): A Cross-Sectional Study

Öztürk¹,

Çelik²,

Çelik³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryThis Cross-Sectional present study was conducted to determine the occurence of aflatoxin M1 (AFM1) in samples of Turkish Republic of North Cyprus (TRNC) traditional cheese (halloumi). In the current study a total of 128 halloumi cheese samples including 36 industrial made and 92 home made were selected by using cluster sampling method and analyzed for AFM1 with the competitive ELISA. The percent of AFM1 contamination in halloumi cheese was found to be low, since 28.8% (0-16.66 ng/kg) of the samples were positive in industrial made and 21.7% (0-4.63 ng/kg) in home made. The mean, standard error of mean (SEM), 95% Confidence Interval (95% CI) values of aflatoxin M1 in halloumi cheese with industrial and home made samples were 0.84±0.24, (95% CI :0.35-1.35) and 1.28±0.32, (95% CI: 0.63-1.93) respectively. Both means were not significantly difference (P=0.422) and found very low from the limits of European Commission (EC) (250 ng/kg) and Turkish Food Codex (TFC) (500 ng/kg) (P<0.001). In order to prevent from introduction of aflatoxin M1 into cheese industry cycle, hygienic conditions, appropriate storage and control of livestock feed at all stages of planting and requires system that makes aflatoxin control are necessary. Keywords: Halloumi cheese, Aflatoxin M1, Cheese, Enzyme-linked immunosorbent assay, ELISA Kıbrıs Geleneksel Peynir (Hellim) Örneklerinde Aflatoksin M1 (AFM1) Oluşumunun Belirlenmesi: Kesitsel Bir Çalışma ÖzetBu kesitsel çalışma ile Kıbrıs geleneksel peynir (Hellim) örneklerinde aflatoksin M1 (AFM1) oluşumunu belirlemek için yapılmıştır. Çalışmada küme örnekleme yöntemiyle 36 endüstriyel yapımı ve 92 ev yapımı olmak üzere toplam 128 Hellim peyniri örneğe seçildi. Peynir örneklerindeki AFM1 varlığı ELISA yöntemi ile analiz edilerek belirlendi. Endüstri yapımı Hellim peynirinde AFM1 pozitif bulunma oranı %28.8 (0.00-16.66 ng/kg), ev yapımı Hellim peynirinde ise bu oran %21.7 (0.00-4.63 ng/kg) şeklinde düşük bir oran olduğu belirlendi. Endüstri ve ev yapımı Hellim peynirindeki AFM1 değerinin ortalama, standart hata (SEM) ve %95 güven aralığında sırasıyla 0.84±0.24, (%95 CI: 0.35-1.35) ve 1.28±0.32, (%95 CI: 0.63-1.93) şeklinde bulundu. Her iki ortalama arasındaki farklılık önemli bulunmadı (P=0.422) ve European Commission (EC) (250 ng/kg) and Turkish Food Codex (TFC) (500 ng/kg) değerlerinden önemli derecede düşük olduğu bulundu (p<0.001). Aflatoksin M1 'in peynirin endüstri döngüsündeki girişini önlemek için, hijyenik koşullar, uygun depolama ve hayvan yemlerinin tüm aşamalarda kontrolünün sağlanması ve aflatoxin düzeyini denetleyen bir sistemin olması gerektiği bilinmelidir.

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Section: Discussionmentioning

confidence: 95%

“…The assay was performed according to the manufacturer's recommendation and as described elsewhere [25] . The mean lower detection limit of the assay was 5 ng/l.…”

Section: Methods Of Analysismentioning

confidence: 99%

To Determine the Occurence of Aflatoxin M1 (AFM1) in Samples of Cyprus Traditional Cheese (Halloumi): A Cross-Sectional Study

Öztürk¹,

Çelik²,

Çelik³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…On the other hand, commercial ELISA kits have been widely used to study the fate of AFM1 during cheese-making or the occurrence of the toxin in various cheeses by several authors. Lopez et al evaluate the performance of one of these commercial kits (Ridascreen Aflatoxin M1, R-Biopharm, Darmstadt, Germany) in the determination of the target compound in cheese and validate it by comparison with a thin layer chromatographic reference method, according to AOAC (Lopez et al, 2001). A nice approach for the rapid detection of AFM1 in milk, which exploits components of an immunoassay, carried out "on-column" instead of in a microtitre plate, was proposed by Sibanda et al (1999).…”

Section: Rapid Techniques For Measuring Afm1 In Cheesementioning

confidence: 99%

“…In addition, sample manipulation often involves the use of hazardous chemicals and laboratory equipment (centrifuge, evaporation systems, etc). As a typical example, the extraction protocol required before measuring the target toxin by means of the Ridascreen ELISA kit (R-Biopharm, Germany), which is the most widely used in AFM1 monitoring in cheese (Tekinsen & Eken, 2008;Colak, 2007;Dashti et al, 2009;Amer & Ibrahim, 2010;Sarimehmetoglu et al, 2004;Lopez et al, 2001;Virdis et al, 2008;Yapar et al, 2008;Ardic et al, 2009;Gurbay et al, 2006;Fallah et al, 2009), consists of the following procedures:…”

Section: Extraction Of Afm1 From Cheese Samples To Be Analysed By Rapmentioning

confidence: 99%

Occurrence of Aflatoxin M1 in Dairy Products

Anfossi¹,

Baggiani²,

Giovannoli³

et al. 2011

Aflatoxins - Detection, Measurement and Control

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“…A number of survey and monitoring programs have been carried out in several countries to obtain general pattern of extent of milk, cheese and yoghurt contamination (AYCICEK et al, 2005;BARRIOS et al, 1996;CARVAJAL et al, 2003;DRAGACCI & FREMY, 1996;ELGERBI et al, 2004;GALVANO et al, 2001;GÜRBAY et al, 2006;KAMBAR, 2005KAMBAR, , 2006KIM et al, 2000;LÓPEZ et al, 2001;MARTINS & MARTINS, 2004;NAKAJIMA et al, 2004;ORUC et al, 2006;ROUSSI et al, 2002;SRIVASTAVA et al, 2001;TEKINSEN & TEKINSEN, 2005).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of aflatoxin M1 in parmesan cheese consumed in Minas Gerais, Brazil

et al. 2008

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RESUMOAflatoxina M 1 (AFM 1 ) pode ocorrer em leite e produtos de leite, resultante da ingestão de aflatoxina B 1 presente em rações de gado leiteiro. Um total de 88 amostras de queijo Parmesão, comercializadas no estado de Minas Gerais, Brasil, no período de março de 2004 a dezembro de 2004, foram analisadas para aflatoxina M 1 por cromatografia líquida de alta eficiência (CLAE) com detector de fluorescência, precedida de purificação da amostra por coluna de imunoafinidade. AFM 1 foi detectada em 40 das 88 amostras (46,4%). Entretanto, somente duas amostras entre estas estavam contaminadas em um nível acima do limite máximo permitido (250 ng kg -1 ), aceito pela Comunidade Européia para AFM 1 . O Brasil não apresenta tolerância máxima permitida para AFM 1 em queijo.Termos para indexação: Aflatoxina M 1 , queijo, ocorrência. ABSTRACTAflatoxin M 1 (AFM 1 ) may occur in milk and milk products, resulting from the ingestion of aflatoxin B 1 in feedstuffs by dairy cow. A total of 88 samples of Parmesan cheese marketed in Minas Gerais state, Brazil, from March 2004 to December 2004, were analyzed for AFM 1 by high-performance liquid chromatography (HPLC) with a fluorescence detector following sample clean-up using immunoaffinity columns. AFM 1 was detected in 40 of the 88 samples (46.4%). However, only two samples among these were contaminated at a level above the maximum permissible limit (250 ng kg -1 ) accepted by European Union for AFM 1. No AFM 1 maximum tolerance limit in cheese has been established in Brazil.

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Distribution of aflatoxin M1 in cheese obtained from milk artificially contaminated

Cited by 79 publications

References 19 publications

To Determine the Occurence of Aflatoxin M1 (AFM1) in Samples of Cyprus Traditional Cheese (Halloumi): A Cross-Sectional Study

To Determine the Occurence of Aflatoxin M1 (AFM1) in Samples of Cyprus Traditional Cheese (Halloumi): A Cross-Sectional Study

Occurrence of Aflatoxin M1 in Dairy Products

Occurrence of aflatoxin M1 in parmesan cheese consumed in Minas Gerais, Brazil

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