Distribution of Antimicrobial Resistance in <i>Campylobacter</i> Strains Isolated from Poultry at a Slaughterhouse and Supermarkets in Japan

Abstract

Fifty strains of Campylobacter jejuni/coli were detected in 108 specimens of chicken meat and organs sampled at six supermarkets and one poultry slaughterhouse (large scale) between April and October 2013 (isolation rates: 84.8% from the slaughterhouse, 29.3% from the supermarkets). 46/50 strains were successfully recovered and subjected to the E-test to examine their susceptibility to three fluoroquinolone antibacterial agents authorized for use in poultry in Japan: enrofloxacin (ERFX), ofloxacin (OFLX), and norfloxacin (NLFX). 29 isolates (63%) were resistant to all three agents and 2 isolates (4.3%) were resistant to two agents (ERFX and OFLX). The resistance rates of strains isolated fom the supermarkets and slaughterhouse were 61.9% and 72.0%, respectively. Because the chickens processed at the slaughterhouse were raised without the use of fluoroquinolone, the results did not suggest a positive relationship between the use of these agents and the distribution of antimicrobial-resistant bacteria. Susceptibility to macrolide antibiotics (erythromycin [EM]) was also tested in 42 strains, and one strain (2.4%), C. coli from a retailer sample, showed resistance. Previous studies have detected high rates of fluoroquinolone-resistant strains, suggesting an expanding distribution of resistant bacteria. The detection of EM-resistant bacteria downstream in the food distribution chain (i.e., closer to consumers) is a concern for human health.

Highly Prevalent Multidrug-Resistant Campylobacter spp. Isolated From a Yellow-Feathered Broiler Slaughterhouse in South China

“…The separation rate of Campylobacter spp. from the slaughterhouse reached a low point of 14.2% in this study, compared with previous reports from white-feathered broiler or turkey slaughterhouse ranging from 26.3 to 100% in China and some other Asian and African countries ( Chen et al, 2010 ; Ma et al, 2014 ; Messad et al, 2014 ; Kojima et al, 2015 ; Han et al, 2016 ). The low separation rate that was obtained may be due to undivided slaughter with the use of disinfectants to reduce the level of cross-contamination or the sample collection methods, seasonal factors ( Zendehbad et al, 2015 ), and the strict biosecurity measures used by the slaughterhouse ( Sasaki et al, 2014 ), may also account for their viable but non-culturable (VBNC) state.…”

A Historical Review on Antibiotic Resistance of Foodborne Campylobacter

“…A controversial avenue for the introduction of antibiotic-resistant Campylobacter strains to humans is through the consumption of meat, including poultry (Thakur et al, 2010; Barton, 2014; US Food and Drug Administration, 2014; Mäesaar et al, 2016). Campylobacter is present as a commensal organism in the gastrointestinal tracts (GITs) of poultry and can be antibiotic resistant (Zhao et al, 2010; Kojima et al, 2015; Ohishi et al, 2017; Raeisi et al, 2017). A Polish study indicated that as many as 94.4% of Campylobacter jejuni isolated from chicken are resistant to at least one class of antibiotic (Wieczorek et al, 2017).…”

Preincubation of <i>Escherichia coli</i> ATCC 25922 with NaCl Increases Its Attachment to Lettuce Surfaces Compared with Other Chemicals

“…Outbreaks of food-borne diseases have been increasingly linked to raw and minimally processed fruits and vegetables; the number of reports examining the attachment of bacterial cells to food has also been growing Kim et al, 2015;Kojima et al, 2015;Kroupitski et al, 2009;Moon et al, 2007;Saggers et al, 2008. Takeuchi et al 2000 evaluated the attachment of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica subsp.…”