Distribution of antimicrobial‐resistant lactic acid bacteria in natural cheese in Japan

Ishihara, Katsuya; Nakajima, Kumiko; Kishimoto, Satoko; Atarashi, Fumiaki; Muramatsu, Yasukazu; Hotta, Akitoyo; Ishii, Shinya; Takeda, Yasuyuki; Kikuchi, Masanori; Tsutsumi, Yutaka

doi:10.1111/1348-0421.12090

Cited by 8 publications

(10 citation statements)

References 26 publications

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“…Moderate level of erythromycin (63/105, 60%) and tetracycline resistance (38/105, 36.19%) reported in present study is in agreement with many of the previous studies (Lonkar et al, 2005;Egervärn et al, 2009;Karapetkov et al, 2011;Ishihara et al, 2013;Dec et al, 2015Dec et al, , 2017 Erythromycin resistance is generally encoded by erm(A), erm(B) and erm(C) while resistance to tetracycline by tet(M), tet(K), tet(S), tet(L) and tet(O) (Egervärn et al, 2009;Dec et al, 2015Dec et al, , 2017. Resistance genes including erm(A), erm(B), erm(C), tet(M), tet(K), tet(S) and tet (O) have been detected from lactobacilli isolated from poultry gut, fermented foods and human gut in previous studies from different parts of the world (Karapetkov et al, 2011;Nawaz et al, 2011;Ishihara et al, 2013;Carrique-Mas et al, 2015;Dec et al, 2015Dec et al, , 2017van Schaik, 2015;). Although, erm(B) or tet(M) have also been successfully amplified from pathogenic bacteria (Farid et al, 2015).…”

Section: Discussionsupporting

confidence: 93%

Phenotypic and Molecular Analysis of Antibiotic Resistance in Lactobacilli of Poultry Origin from Lahore, Pakistan

Saleem¹

2018

PVJ

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Acquired antibiotic resistance in lactobacilli poses a significant threat to public health. This study presents phenotypic and molecular analysis of antibiotic resistance in lactobacilli of poultry gut origin. A total of 105 Lactobacillus spp. isolates including 59 from indigenous and 46 from commercial poultry were recovered. Lactobacilli were identified by genus specific polymerase chain reaction (PCR) targeting 16SrDNA-23S rDNA inter-spacer region. Minimum inhibitory concentrations of different antibiotics were determined by broth micro dilution method. Antibiotic resistance genes for erythromycin [erm(B)] and tetracycline [tet(M)] were amplified by PCR and sequenced for homology analysis. Antibiotic resistance against cephradine (67.39% vs 55.93%), cefuroxime (100% vs 77.96%), erythromycin (86.95% vs 38.98%), tetracycline (41.30% vs 32.2%), ciprofloxacin (91.30% vs 84.74%), levofloxacin (97.82% vs 81.35%) and ofloxacin (95.65% vs 88.13%) were comparatively higher in lactobacilli of commercial poultry origin as compared to indigenous poultry. Resistance against ampicillin was slightly higher in back yard (49.15%) as compared to commercial poultry (43.47%). Overall, a high level resistance against cefuroxime (87.61%), levofloxacin (88.57%), ofloxacin (91.42%), ciprofloxacin (87.61%), and moderate level resistance against cephradine (60.95%), ampicillin (46.66%), tetracycline (36.19%) and erythromycin (60%) was observed. Erythromycin and tetracycline resistant genes [erm (B), and tet(M), respectively] were successfully amplified from phenotypically resistant lactobacilli. Sequencing analysis revealed that erm(B) gene had >99% similarity with erm(B) gene of Enterococcus faecium while tet(M) had >99% similarity with tet(M) of E. coli. It is concluded that lactobacilli of poultry gut origin contain acquired antibiotic resistance and its transmission to other bacterial strains is a significant threat to public health.

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Section: Discussionsupporting

confidence: 93%

Phenotypic and Molecular Analysis of Antibiotic Resistance in Lactobacilli of Poultry Origin from Lahore, Pakistan

Saleem¹

2018

PVJ

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“…The percentage of enrofloxacin-resistant Lactobacillus strains (MIC≥64 μg/ml) in the turkeys (60%) was higher than in chickens (48%) and in geese (23%) in Poland [ 16 , 45 ]. Lactobacilli from other sources, such as dairy products or cattle intestine, are usually sensitive to enrofloxacin [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and antibiotic susceptibility of lactobacilli isolated from turkeys

et al. 2018

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BackgroundThe aim of this study was to identify Lactobacillus isolates derived from turkeys from six Polish farms and to characterize their phenotypic and genotypic antibiotic resistance profiles.ResultsAmong 62 isolates identified by MALDI-TOF mass spectrometry and restriction analysis of 16S rDNA, the dominant species was L. salivarius (35%), followed by L. crispatus (21%), L. ingluviei (14.5%) and L. johnsonii (10%). A high prevalence of resistance to tetracycline (68% resistant isolates), lincomycin (64.5%) and enrofloxacin (60%) among the lactobacilli tested was observed. Fewer than 50% isolates were resistant to ampicillin (47%), erythromycin (45%), streptomycin (31%), chloramphenicol (29%) and gentamicin (10%). As many as 64,5% of the isolates showed multidrug resistance. High MIC values for ampicillin (≥64 μg/ml) were usually accompanied by elevated MICs for cephalosporins (≥16 μg/ml) and high MICs for tiamulin, i.e. ≥32 μg/ml, were noted in most of the turkey lactobacilli (61%). The occurrence of resistance genes was associated with phenotypic resistance, with the exception of five phenotypically susceptible isolates that contained the tetM, tetL, ermC, ermB or cat genes. The most frequently identified were ermB (45% isolates), tetL (40%), tetW (37%) and tetM (29%), and the occurrence of lnuA (18%), cat (10%), ermC (6%), ant(6)-Ia (5%) and aadE (5%) was less frequent. The mechanism of ampicillin resistance has not been elucidated, but the results of nitrocefin test confirmed that it is not involved in the production of beta-lactamases.ConclusionsThe high rate of antibiotic resistance observed in this study indicates the need to implement the principles of rational use of antibiotics in poultry. The presence of transmissible resistant genes in lactobacilli may contribute to the development of antibiotic resistant pathogenic strains that pose a threat to both poultry and consumers. The results of these studies may be useful for committees providing guidance on antibiotic susceptibility of microorganisms in order to revise and supplement current microbiological cut-offs values within the genus Lactobacillus.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1269-6) contains supplementary material, which is available to authorized users.

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“…Other authors have reported greater susceptibility of LAB to enrofloxacin. The MIC range noted by Ishihara et al [ 54 ] for lactobacilli isolated from dairy products was 1–8 μg/ml, and in the case of bovine lactobacilli MIC ≥64 μg/ml was noted for only 3% of isolates [ 44 ]. The presence of low MIC values for enrofloxacin, i.e.…”

Section: Discussionmentioning

confidence: 99%

Assessment of antibiotic susceptibility in Lactobacillus isolates from chickens

et al. 2017

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BackgroundThe aim of this study was to determine the susceptibility of 88 Lactobacillus isolates derived from chickens to antibiotic substances and to detect drug-resistance genes.ResultsThe minimal inhibitory concentration of 13 antimicrobial substances was determined by the broth microdilution method, and resistance genes were detected by PCR. We recorded a high prevalence of resistance to tiamulin (90% resistant isolates), tetracyclines (74%) and lincomycin (70%), and a moderately high frequency of resistance to enrofloxacin (48%), macrolides (42%), aminoglycosides (12.5–31%), ampicillin (26%) and chloramphenicol (23%). Multi-drug resistance was observed in 79.5% of isolates. The presence of resistance genes was generally correlated with phenotypic resistance, but some molecular determinants were also recorded in susceptible isolates. Among tetracycline resistance genes, the most frequently identified was tetW (45% isolates), followed by tetM (26%) and tetL (24%). The ermB, ermC and lnuA genes, associated with resistance to macrolides and lincosamides, were observed in 39, 12 and 39% of isolates, respectively. Among genes determining resistance to aminoglycoside antibiotics, we identified ant(6)-Ia (10% of isolates), aac(6′)-Ie-aph(2′)-Ia (8%), aph(2″)-Ic (6%) and aadE (4.5%). The cat gene was present in 32 isolates, including 8 of 20 found to be resistant to chloramphenicol. Two genes encoding efflux pumps were identified—the acrA gene was present in all isolates tested, and 10 of 79 lactobacilli determined to be phenotypically resistant to tiamulin contained the lsaE gene. We were unable to explain the resistance mechanism of Lactobacillus isolates to ampicillin, but showed that it did not involve the production of β-lactamases.ConclusionsOur findings indicate that intestinal lactobacilli should be considered a reservoir of resistance genes and that antibiotics must be used prudently in poultry production. The data derived from this study can be used as a basis for reviewing current microbiological breakpoints for categorization of susceptible and resistant strains within the genus Lactobacillus.

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Distribution of antimicrobial‐resistant lactic acid bacteria in natural cheese in Japan

Cited by 8 publications

References 26 publications

Phenotypic and Molecular Analysis of Antibiotic Resistance in Lactobacilli of Poultry Origin from Lahore, Pakistan

Phenotypic and Molecular Analysis of Antibiotic Resistance in Lactobacilli of Poultry Origin from Lahore, Pakistan

Identification and antibiotic susceptibility of lactobacilli isolated from turkeys

Assessment of antibiotic susceptibility in Lactobacillus isolates from chickens

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