Distribution of Atmospheric Methane Oxidation and Methanotrophic Communities on Hawaiian Volcanic Deposits and Soils

King, Gary M.; Nanba, Kenji

doi:10.1264/jsme2.me08529

Cited by 22 publications

(30 citation statements)

References 40 publications

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“…3 and 4) that might represent PmoA or AmoA sequences. Cluster 6 includes the environmental sequence EU723753 (retrieved from a forest soil) (26). The novel cluster 7 exclusively contains sequences obtained from Solling beech soil samples.…”

Section: Resultsmentioning

confidence: 99%

Different Atmospheric Methane-Oxidizing Communities in European Beech and Norway Spruce Soils

Degelmann¹,

Borken

Drake³

et al. 2010

Appl Environ Microbiol

145

123

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Norway spruce (Picea abies) forests exhibit lower annual atmospheric methane consumption rates than do European beech (Fagus sylvatica) forests. In the current study, pmoA (encoding a subunit of membranebound CH 4 monooxygenase) genes from three temperate forest ecosystems with both beech and spruce stands were analyzed to assess the potential effect of tree species on methanotrophic communities. A pmoA sequence difference of 7% at the derived protein level correlated with the species-level distance cutoff value of 3% based on the 16S rRNA gene. Applying this distance cutoff, higher numbers of species-level pmoA genotypes were detected in beech than in spruce soil samples, all affiliating with upland soil cluster ␣ (USC␣). Additionally, two deep-branching genotypes (named 6 and 7) were present in various soil samples not affiliating with pmoA or amoA. Abundance of USC␣ pmoA genes was higher in beech soils and reached up to (1.2 ؎ 0.2) ؋ 10 8 pmoA genes per g of dry weight. Calculated atmospheric methane oxidation rates per cell yielded the same trend. However, these values were below the theoretical threshold necessary for facilitating cell maintenance, suggesting that USC␣ species might require alternative carbon or energy sources to thrive in forest soils. These collective results indicate that the methanotrophic diversity and abundance in spruce soils are lower than those of beech soils, suggesting that tree species-related factors might influence the in situ activity of methanotrophs.

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Section: Resultsmentioning

confidence: 99%

Different Atmospheric Methane-Oxidizing Communities in European Beech and Norway Spruce Soils

Degelmann¹,

Borken

Drake³

et al. 2010

Appl Environ Microbiol

145

123

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“…Recovery of the diversity of methanotroph communities has been observed in sites where plant diversity was increased (Knief et al, 2005;King and Nanba, 2008;Zhou et al, 2008;Degelmann et al, 2010;Dorr et al, 2010), perhaps due to the differential production of monoterpenes (Maurer et al, 2008;Degelmann et al, 2010), suggesting a role for plant diversity in shaping methanotroph communities. Additionally, nitrogen fertilization is known to change the structure of methanotroph communities (Lau et al, 2007;Maxfield et al, 2008), most likely through competitive inhibition of methane monooxygenase by ammonia (Gulledge and Schimel, 1998), although it can stimulate CH 4 consumption under conditions where fixed nitrogen is limiting methanotroph metabolism (Bodelier and Laanbroek, 2004).…”

Section: Discussionmentioning

confidence: 99%

Agriculture's impact on microbial diversity and associated fluxes of carbon dioxide and methane

et al. 2011

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Agriculture has marked impacts on the production of carbon dioxide (CO 2 ) and consumption of methane (CH 4 ) by microbial communities in upland soils-Earth's largest biological sink for atmospheric CH 4 . To determine whether the diversity of microbes that catalyze the flux of these greenhouse gases is related to the magnitude and stability of these ecosystem-level processes, we conducted molecular surveys of CH 4 -oxidizing bacteria (methanotrophs) and total bacterial diversity across a range of land uses and measured the in situ flux of CH 4 and CO 2 at a site in the upper United States Midwest. Conversion of native lands to row-crop agriculture led to a sevenfold reduction in CH 4 consumption and a proportionate decrease in methanotroph diversity. Sites with the greatest stability in CH 4 consumption harbored the most methanotroph diversity. In fields abandoned from agriculture, the rate of CH 4 consumption increased with time along with the diversity of methanotrophs. Conversely, estimates of total bacterial diversity in soil were not related to the rate or stability of CO 2 emission. These combined results are consistent with the expectation that microbial diversity is a better predictor of the magnitude and stability of processes catalyzed by organisms with highly specialized metabolisms, like CH 4 oxidation, as compared with processes driven by widely distributed metabolic processes, like CO 2 production in heterotrophs. The data also suggest that managing lands to conserve or restore methanotroph diversity could mitigate the atmospheric concentrations of this potent greenhouse gas.

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“…Methane oxidizers in natural Podzoluvisol, Luvisol, and gleyic Kastanozem were distantly related to uncultured methanotrophs from Hawaiian forest soil [45], rice field soils [46], and soils in Greenland [47]. The novel pmoA/amoA cluster detected in the investigated soils was distantly related to known pmoA or amoA genes (Figure 2).…”

Section: Diversity Of Methanotrophs In Unmanaged and Managed Soilsmentioning

confidence: 92%

Aerobic Methanotrophs in Natural and Agricultural Soils of European Russia

2013

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Human activities such as land management and global warming have great impact on the environment. Among changes associated with the global warming, rising methane emission is a serious concern. Therefore, we assessed methane oxidation activity and diversity of aerobic methanotrophic bacteria in eight soil types (both unmanaged and agricultural) distributed across the European part of Russia. Using a culture-independent approach targeting pmoA gene, we provide the first baseline data on the diversity of methanotrophs inhabiting most typical soil types. The analysis of pmoA clone libraries showed that methanotrophic populations in unmanaged soils are less diverse than in agricultural areas. These clone sequences were placed in three groups of, so far, uncultured methanotrophs: USC-gamma, cluster I, and pmoA/amoA cluster, which are believed to be responsible for atmospheric methane oxidation in upland soils. Agricultural soils harbored methanotrophs related to genera Methylosinus, Methylocystis, Methylomicrobium, Methylobacter, and Methylocaldum. Despite higher numbers of detected molecular operational taxonomic units (MOTUs), managed soils showed decreased methane oxidation rates as observed in both in situ and laboratory experiments. Our results also suggest that soil restoration may have a positive effect on methane consumption by terrestrial ecosystems.

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Distribution of Atmospheric Methane Oxidation and Methanotrophic Communities on Hawaiian Volcanic Deposits and Soils

Cited by 22 publications

References 40 publications

Different Atmospheric Methane-Oxidizing Communities in European Beech and Norway Spruce Soils

Different Atmospheric Methane-Oxidizing Communities in European Beech and Norway Spruce Soils

Agriculture's impact on microbial diversity and associated fluxes of carbon dioxide and methane

Aerobic Methanotrophs in Natural and Agricultural Soils of European Russia

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