Distribution of B-cell epitopes on the pseudorabies virus glycoprotein B.

Abstract

In order to map antigenically important regions of glycoprotein B (gB) of pseudorabies virus (PrV), a panel of recombinant fragments of gB expressed in E. coli and truncated fragments of gB generated by cleavage of purified native gB with trypsin and cyanogen bromide was analysed by using 26 monoclonal antibodies directed against gB. Three continuous epitopes were localized in the vicinity of the N terminus of gB, between amino acids (aa) 59 and 126. One continuous epitope mapped between residues 214 and 279. The residues involved in the assembly of eight discontinuous epitopes were located between aa 540 and 734. The constituents of two discontinuous epitopes were harboured in a segment encompassing aa 540-646. The clustering of continuous epitopes at the extreme N terminus of PrV gB and the locations of residues involved in the assembly of discontinuous epitopes of PrV gB are in good agreement with data on epitope locations in gB homologues from other herpesviruses.

Differential segregation of protective immunity by encoded antigen in DNA vaccine against pseudorabies virus

“…These glycoproteins are involved in the essential steps in the progress of viral infection 1 . Several B‐cell epitopes detected on PrV gB and PrV gC glycoproteins 10 , 11 , 12 , 13 and T‐cell epitopes detected on PrV gC 12 , 13 induce both humoral and cytotoxic responses 4 , 14 . PrV gD induces the production of strongly neutralizing antibodies and weak cytotoxic responses 14 .…”

Importance of M-Protein C Terminus as Substrate Antigen for Serodetection of Equine Arteritis Virus Infection

“…The use of procaryote-derived fusion proteins has provided a rapid and reliable tool for the determination of the antigenic structure of several viral proteins (27,35). Here, the iPCR procedure was used to introduce selected deletions within the M-protein amino acid sequence in a single experimental step, avoiding the use of subcloning procedures to generate mutant proteins.…”

Development of Dual Fluorescent Microsphere Immunological Assay for Detection of Pseudorabies Virus gE and gB IgG Antibodies

“…It can induce homotypic T cell aggregation and transmit the virus to highly susceptible cells by activating extracellular signal-regulated kinase 1/2 signaling pathways [12,13]. A major epitope in the gE amino acids (aa) 52-238 domain and eight epitopes in the gB aa 539-741 domain were identi ed [14,15]. Generally, vaccination is a valuable method to control PRV.…”