Distribution of basal lamina type IV collagen and laminin in normal rat tongue mucosa and experimental oral carcinoma: Ultrastructural immunolocalisation and immunogold quantitation

Dejun, Jiang; Wilson, David; Smith, Peter J. S.; Pierce, Angela; Wiebkin, Ole W.

doi:10.1016/0964-1955(94)90004-3

Cited by 11 publications

(11 citation statements)

References 34 publications

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“…Destruction of the extracellular matrix was extensive in s.c.c., with very little matrix remaining in advanced s.c.c. These results are in agreement with histological and immunohistochemical studies linking the carcinogenesis process with progressive decreases in extracellular matrix organization and presence [6][7][8][9].…”

Section: Discussionsupporting

confidence: 81%

“…The neoplastic process is associated with changes in the extracellular matrix (ECM), especially the collagen fibers and the framework that they constitute. Several authors have described collagen loss in malignancy, although the time-and spatially-resolved sequence of this process remains undefined [5][6][7][8][9]. Angiogenesis and altered tissue perfusion and oxygenation are also implicated in neoplasia, but again, the specific sequential, spatial, and functional relationship between vascularization and neoplasia has not been clearly elucidated [5,6].…”

Section: Introductionmentioning

confidence: 99%

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In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy

et al. 2004

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Background and Objective: Current techniques for oral diagnosis require surgical biopsy of lesions, and may fail to detect early malignant change. Non-invasive, sensitive tools providing early detection of oral cancer and a better understanding of malignant change are needed. These studies evaluated in vivo multiphoton excited fluorescence (MPM) techniques to (1) map epithelial and subepithelial changes through out oral carcinogenesis and (2) serve as an effective diagnostic modality. Study Design/Materials and Methods: In the hamster model (n ¼ 70), epithelial and subepithelial change was imaged in vivo throughout carcinogenesis. MPM-and histopathology-based diagnoses on a scale of 0 (healthy)-6 (squamous cell carcinoma [s.c.c.]) were scored by two prestandardized investigators. Results: Collagen matrix and fibers, cellular infiltrates, blood vessels, and microtumors were clearly visible. MPM agreed with the histopathology for 88.6% of diagnoses. Conclusions: In vivo MPM images provide (1) high resolution information on specific components of the carcinogenesis process (2) an excellent basis for oral diagnostics.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy

et al. 2004

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“…The immunohistochemical identification of laminin and Type IV collagen in normal and cancerous rat tissues has also been established using light microscopy and the avidin-biotin peroxidase technique 8 and subsequently by both routine 7 and quantitative immunogold electron microscopy. 17,18 These latter techniques employed standard IgG-gold and Protein A-gold postembedding incubations. membrane ( Fig.…”

Section: B) Immunohistochemistrymentioning

confidence: 99%

Oral cancer: Role of the basement membrane in invasion

Wilson

Dejun

Pierce

et al. 1999

Australian Dental Journal

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Invasive growth of cancer cells is a complex process involving specific interactions between tumour cells and the orderly, integrated complexes of the extracellular matrix. Basement membranes have been proposed as one constituent of extracellular matrix which carries responsibility for regulating invasion and metastasis. Using a chemically induced rat tongue carcinoma model, it has been shown that components of the basement membrane and its overall structure are altered during tumour invasion, and methods have been developed to quantitate some of these differences. Since the basement membrane can be specifically characterized by its fibrous protein network of Type IV collagen and laminin, which is embedded in a heparan sulphate-rich proteoglycan matrix, these components have been targeted. In particular, the current paper presents results in the context of current concepts of early changes in neoplastic invasion of underlying connective tissues. In consequence, further elaboration of the underlying mechanisms of epithelial migration in oral cancer may allow an exploration of the use of alterations in expression of basement membrane components as prognostic indicators.

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“…15 Jiang et al (1994) 16 and Harada et al (1994) 17 have demonstrated this finding by observing lower laminin expression in metastatic tumors. In our study, of 12 high malignancy lesions, two did not express laminin, while discrete intensity marking was seen in another 8 lesions.…”

Section: Discussionmentioning

confidence: 84%

Expressão da laminina na membrana basal em carcinoma escamocelular oral

Souza

Silva

et al. 2007

Rev. Bras. Otorrinolaringol.

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The basement membrane is a dynamic structure that undergoes quantitative and qualitative changes during the progression of squamous cell carcinoma, which is essencially important in tumoral invasion and metastasis. Aim: This study is aimed at investigating the behavior of the basement membrane in oral squamous cell carcinomas with different malignancy scores, which were obtained through the immunohistochemical expression of the laminin, a glycoprotein present in the basement membrane. Study design: History cross-sectional cohort. Material and method: Thirty-one cases of oral squamous cell carcinoma were subjected to histological grading of malignant tumors. The immunohistochemical expression of the laminin in lesions bearing different scores of malignancy was evaluated according to intensity and integrity, using the StreptavidinBiotin complex method. Results: We noticed significant differences in the media between intensity and continuity laminin expression in relation to different grades of malignancy. Conclusion: Different expressions of laminin, a glycoprotein present in basement membranes were evident in oral cell carcinomas within different grades of histological malignancy.

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Distribution of basal lamina type IV collagen and laminin in normal rat tongue mucosa and experimental oral carcinoma: Ultrastructural immunolocalisation and immunogold quantitation

Cited by 11 publications

References 34 publications

In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy

In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy

Oral cancer: Role of the basement membrane in invasion

Expressão da laminina na membrana basal em carcinoma escamocelular oral

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