Distribution of biocide resistant genes and biocides susceptibility in multidrug-resistant Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii — A first report from the Kingdom of Saudi Arabia

Vijayakumar, Rajendran; Sandle, Tim; Al-Aboody, Mohammad Saleh; AlFonaisan, Meshal K.; Alturaiki, Wael; Mickymaray, Suresh; Premanathan, Mariappan; Alsagaby, Suliman A.

doi:10.1016/j.jiph.2018.05.011

Cited by 74 publications

(62 citation statements)

References 29 publications

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“…In our study, the qacEdelta and qacE genes have been more frequently associated with wastewater isolates, suggesting the selection of this type of resistance in the aquatic environment, probably due to the high selection pressure exerted by the presence of disinfectants. The qacdeltaE and qacE were isolated from a class I integron in the R751 plasmid, and were first documented in K. pneumoniae [69]. The qacE gene was, as expected, less encounteredsince it is predominantly associated with Gram-positive bacteria [70].…”

Section: Discussionmentioning

confidence: 66%

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Surleac

Barbu

Paraschiv³

et al. 2020

PLoS ONE

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We report on the genomic characterization of 47 multi-drug resistant, carbapenem resistant and ESBL-producing K. pneumoniae isolates from the influent (I) and effluent (E) of three wastewater treatment plants (WWTPs) and from Romanian hospital units which are discharging the wastewater in the sampled WWTPs. The K. pneumoniae whole genome sequences were analyzed for antibiotic resistance genes (ARGs), virulence genes and sequence types (STs) in order to compare their distribution in C, I and E samples. Both clinical and environmental samples harbored prevalent and widely distributed ESBL genes, i.e. bla SHV , bla OXA , bla TEM and bla CTX M . The most prevalent carbapenemase genes were bla NDM-1 , bla OXA-48 and bla KPC-2 . They were found in all types of isolates, while bla OXA-162 , a rare bla OXA-48 variant, was found exclusively in water samples. A higher diversity of carbapenemases genes was seen in wastewater isolates. The aminoglycoside modifying enzymes (AME) genes found in all types of samples were aac(6'), ant(2'') Ia, aph(3'), aaD, aac(3) and aph(6). Quinolone resistance gene qnrS1 and the multi-drug resistance oqxA/B pump gene were found in all samples, while qnrD and qnrB were associated to aquatic isolates. The antiseptics resistance gene qacEdelta1 was found in all samples, while qacE was detected exclusively in the clinical ones. Trimethroprim-sulfamethoxazole (dfrA, sul1 and sul2), tetracyclines (tetA and tetD) and fosfomycin (fosA6, known to be located on a transpozon) resistance genes were found in all samples, while for choramphenicol and macrolides some ARGs were detected in all samples (catA1 and catB3 / mphA), while other (catA2,

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Section: Discussionmentioning

confidence: 66%

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Surleac

Barbu

Paraschiv³

et al. 2020

PLoS ONE

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“…[143]. Baicalein (44), EGCG (20), galangin (18), kaempferide (48), DL-cycloserine (49), quercetin (1), apigenin (8), and kaempferide-3-O-glucoside (50) (Figure 2) inhibit the synthesis of peptidoglycan, which is an essential component of the bacterial cell wall, resulting in cell wall damage [144][145][146].…”

Section: Inhibition Of Cell Wall Constructionmentioning

confidence: 99%

“…Alkaloids are nitrogenous compounds characterized by their alkaline nature, which aids the inhibition of cell respiration, intercalates with DNA, and inhibits various enzymes involved in replication, transcription, and translation [147]. Plant-based bioactive compounds such as quercetin (1), nobiletin (51), myricetin (43), tangeritin (52,) genistein (31), apigenin (8), chrysin (24), kaempferol (23), and 3, 6, 7, 30, 40-pentahydroxyflavone (39) have been recognized as noteworthy DNA gyrase inhibitors, which are essential for DNA replication in prokaryotes including V. harveyi, B. subtilis, M. smegmatis, M. tuberculosis, and E. coli [146,[148][149][150][151]. These bioactive compounds binding to the β subunit of gyrase and the corresponding blockage of the ATP binding pocket eventually contribute to the antimicrobial activity.…”

Section: Inhibition Of Prokaryotic Dna Replicationmentioning

confidence: 99%

Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens

Mickymaray

2019

Antibiotics

118

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Traditional medicinal plants have been cultivated to treat various human illnesses and avert numerous infectious diseases. They display an extensive range of beneﬁcial pharmacological and health effects for humans. These plants generally synthesize a diverse range of bioactive compounds which have been established to be potent antimicrobial agents against a wide range of pathogenic organisms. Various research studies have demonstrated the antimicrobial activity of traditional plants scientifically or experimentally measured with reports on pathogenic microorganisms resistant to antimicrobials. The antimicrobial activity of medicinal plants or their bioactive compounds arising from several functional activities may be capable of inhibiting virulence factors as well as targeting microbial cells. Some bioactive compounds derived from traditional plants manifest the ability to reverse antibiotic resistance and improve synergetic action with current antibiotic agents. Therefore, the advancement of bioactive-based pharmacological agents can be an auspicious method for treating antibiotic-resistant infections. This review considers the functional and molecular roles of medicinal plants and their bioactive compounds, focusing typically on their antimicrobial activities against clinically important pathogens.

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“…The microplate was covered and incubated at 37ºC for 16 -20 hours after which the turbidity was measured at 625 nm in a microplate reader. The MIC was defined as the lowest concentration of the antiseptic that inhibits visible growth of the tested A. baumannii isolate as measured by the OD625 values compared with the controls [12].…”

Section: Determination Of Mic Values For Antisepticsmentioning

confidence: 99%

“…However, extensive use of these biocides in hospitals had led to concerns on the development of resistance and the spread of biocide resistance genes [10,11], mirroring the spread of antibiotic resistance in pathogenic bacteria. Despite the importance of biocide resistance, there is a dichotomy in the number of published papers regarding reduced susceptibilities to biocides when compared with the much larger volume of publications on antibiotic resistance [12]. In Malaysia, there has only been a single published paper so far that reported on antiseptic resistance in A. baumannii and its associated resistance genes [13].…”

Section: Introductionmentioning

confidence: 99%

Biocide susceptibilities and biofilm-forming capacities of Acinetobacter baumannii clinical isolates from Malaysia

A'shimi

Alattraqchi

Rani

et al. 2019

J Infect Dev Ctries

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Introduction. Acinetobacter baumannii is a Gram-negative nosocomial pathogen that has the capacity to develop resistance to all classes of antimicrobial compounds. However, very little is known regarding its susceptibility to biocides (antiseptics and disinfectants) and capacity to form biofilms, particularly for Malaysian isolates. Aim. To determine the susceptibility of A. baumannii isolates to commonly-used biocides, investigate their biofilm-forming capacities and the prevalence of biocide resistance and biofilm-associated genes. Methodology. The minimum inhibitory concentration (MIC) values of 100 A. baumannii hospital isolates from Terengganu, Malaysia, towards the biocides benzalkonium chloride (BZK), benzethonium chloride (BZT) and chlorhexidine digluconate (CLX), were determined by broth microdilution. The isolates were also examined for their ability to form biofilms in 96-well microplates. The prevalence of biocide resistance genes qacA, qacE and qacDE1 and the biofilm-associated genes bap and abaI were determined by polymerase chain reaction (PCR). Results. Majority of the A. baumannii isolates (43%) showed higher MIC values (> 50 µg/mL) for CLX than for BZK (5% for MIC > 50 µg/mL) and BZT (9% for MIC > 50 µg/mL). The qacDE1 gene was predominant (63%) followed by qacE (28%) whereas no isolate was found harbouring qacA. All isolates were positive for the bap and abaI genes although the biofilm-forming capacity varied among the isolates. Conclusion. The Terengganu A. baumannii isolates showed higher prevalence of qacDE1 compared to qacE although no correlation was found with the biocides’ MIC values. No correlation was also observed between the isolates’ biofilm-forming capacity and the MIC values for the biocides.

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Distribution of biocide resistant genes and biocides susceptibility in multidrug-resistant Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii — A first report from the Kingdom of Saudi Arabia

Cited by 74 publications

References 29 publications

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania

Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens

Biocide susceptibilities and biofilm-forming capacities of Acinetobacter baumannii clinical isolates from Malaysia

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