Distribution of bovine fetuin and albumin in plasma, allantoic and amniotic fluids during development

Lai, Ping-Chin; Huang, Leeyaun; Panrucker, D. E.; Church, Robert B.; Lorscheider, F. L.

doi:10.1530/jrf.0.0630053

Cited by 18 publications

(10 citation statements)

References 20 publications

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“…The pig is the only species so far described in which plasma albumin does not rise consid erably during the last part of gestation [3,5,13,16,28; this paper, table I]. In our experi ments albumin contributed no more than 10% of the total plasma protein concentra tion in the fetus (table I) and we confirm that, as previously reported [36], the albumin con centration around 30 days gestation was very low indeed.…”

Section: Proteins In Fetal Plasmasupporting

confidence: 88%

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Proteins in Cerebrospinal Fluid and Plasma of Fetal Pigs during Development

et al. 1982

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The concentrations of total protein, α-fetoprotein, fetuin, transferrin and albumin have been measured in cerebrospinal fluid (CSF) and plasma of fetal pigs from 26 to 109 days gestation. Plasma total protein concentration was lowest in the youngest fetuses but thereafter it was between 1,500 and 2,200 mg/100 ml. α-Fetoprotein was the predominant plasma protein early in development but its concentration declined throughout the fetal period. α-Fetoprotein, fetuin and transferrin accounted for over 65% of the total protein concentration at all fetal ages. Albumin was not detectable in either plasma or CSF at 26 days and did not account for more than 10% of total plasma protein concentration at later stages of gestation; in contrast fetuin was the major protein in plasma (and CSF) later in fetal development. In CSF the concentration of fetuin was highest (178 ± 8 mg/100 ml) at 26 days. The concentrations of total protein (961 ± 95 mg/100 ml), α-fetoprotein (406 ± 77 mg/100 ml) and transferrin (195 ± 19 mg/100 ml) were highest at 31 days after which the concentration of all the main proteins fell very considerably. Albumin was first detected in CSF at 38 days and contributed less than 10% of total protein concentration at later fetal ages. Possible origins of plasma proteins in fetal CSF are discussed. Differences in CSF: plasma ratios for different proteins are consistent with a specific transfer mechanism between their two compartments; but synthesis by fetal choroid plexus or brain has not been excluded.

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Section: Proteins In Fetal Plasmasupporting

confidence: 88%

“…It would be interesting to know if plasma fe tuin concentration falls very rapidly in the postnatal piglet as has been reported for new born calves [28] in contrast to the rapid rise in albumin [22] and total protein [11,34] which occurs in neonatal piglet serum.…”

Section: Proteins In Fetal Plasmamentioning

confidence: 99%

Proteins in Cerebrospinal Fluid and Plasma of Fetal Pigs during Development

et al. 1982

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“…Two properties attributed to fetuin are relevant to the first aspect: 1) fetuin is a carrier protein for growth factors (Fisher et al, 1958;Graham, 1972;Temin et al, 1972;Lai et al, 1981;Lieberman and Ove, 1981). and 2) fetuin has a protease inhibitory activity (Galembeck and Cann, 1974;Rohrlich and Rifkin, 1981).…”

Section: Discussionmentioning

confidence: 99%

Fetuin: A serum component associated with rat sertoli and spermatogenic cells in coculture

Abdullah

Crowell

Tres

et al. 1986

Journal Cellular Physiology

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Cocultures of rat Sertoli-spermatogenic cells plated in a culture medium supplemented with 10% fetal bovine serum for 6-12 h and then maintained in serum free, hormone/growth factor-supplemented medium accumulated an acidic glycoprotein of molecular weight of 68,000 dalton (68 kD) and isoelectric point range of about 4.2-3.5. Anion exchange chromatography has allowed the partial purification of this protein, which consists of a major protein band of 68 kD and two minor, low molecular weight components. A rabbit antiserum raised against the 68 kD component also crossreacts with the two low molecular weight components, thus suggesting that these two minor components are antigenically related to the 68 kD protein. The 68 kD protein has been identified as fetuin, the major component of fetal bovine serum, based on similar molecular weight, isoelectric point, immunoreactivity and trypsin inhibitory activity. Labeling experiments with [14C]amino acid mixture show that 68 kD protein is not synthesized by cocultured rat Sertoli and spermatogenic cells. Immunocytochemistry and Western blot approaches carried out under various experimental conditions support the view that the fetuin-68 kD protein is taken up from serum by both Sertoli cells and pachytene spermatocytes. Because fetuin 1) behaves as a carrier protein for growth factors, 2) has protease inhibitory activity, 3) is preferentially internalized by Sertoli cells and pachytene spermatocytes and 4) fetal bovine serum-supplemented medium impairs spermatogenic cell viability, there is a need to further define appropriate conditions for optimizing long-term viability and differentiation of spermatogenic cells in vitro.

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“…It should be noted that only a 10% FCS was enough to suppress endogenous virus genome expression in the persistent infection system of MDCK-IVpi cells. Furthermore, the concentration of 1 mg/ml fetuin that causes antiviral eVects is calculated to be equivalent to the fetuin level in 10% FCS [20]. Fetuin has the potential capacity to stimulate cell division of cultured cells in serum-free medium [30].…”

Section: Discussionmentioning

confidence: 99%

Fetal calf serum inhibits virus genome expression in Madin-Darby canine kidney cells persistently infected with influenza A virus

Hossain

Mori

Dong

et al. 2007

Med Microbiol Immunol

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A cell line of Madin-Darby canine kidney (MDCK) cells persistently infected with human influenza A virus has been established and designated as MDCK-IVpi cells. Production of progeny virus in MDCK-IVpi cells was suppressed when the cells were incubated in the presence of 10% fetal calf serum (FCS). FCS impaired virus mRNA synthesis in MDCK-IVpi cells, which resulted in a scarcity of virus proteins for virion formation. However, MDCK-IVpi cells well supported the growth of superinfecting heterologous influenza viruses, even in the presence of FCS. A certain fetuin-like substance in FCS might be responsible for the observed inhibition of virus replication.

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Distribution of bovine fetuin and albumin in plasma, allantoic and amniotic fluids during development

Cited by 18 publications

References 20 publications

Proteins in Cerebrospinal Fluid and Plasma of Fetal Pigs during Development

Proteins in Cerebrospinal Fluid and Plasma of Fetal Pigs during Development

Fetuin: A serum component associated with rat sertoli and spermatogenic cells in coculture

Fetal calf serum inhibits virus genome expression in Madin-Darby canine kidney cells persistently infected with influenza A virus

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