Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Argentina

Volder, A López De; Teves, S.; Isasmendi, Adela; Pinheiro, José Luís Palmeiro; Ibarra, L.; Breglia, Noelia; Herrera, Teófilo; Vazquez, M.; Hernández, C; Degrossi, José

doi:10.1007/s10123-020-00151-z

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…The Burkholderia cepacia complex (Bcc) is a group of at least 24 species that are ubiquitous in nature and are highly problematic opportunistic pathogens ( 1 – 3 ). Consistent with the large range of ecological niches the Bcc occupies, species of the complex are also present in clinical settings and can contaminate medical equipment, including nebulizers, saline solutions, and disinfectants ( 4 – 8 ). The Bcc species cause chronic pulmonary infections, pneumonia, and septicemia in immunocompromised individuals, particularly those with cystic fibrosis (CF) ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

Burkholderia contaminans Bacteriophage CSP3 Requires O-Antigen Polysaccharides for Infection

2023

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Section: Introductionmentioning

confidence: 99%

Burkholderia contaminans Bacteriophage CSP3 Requires O-Antigen Polysaccharides for Infection

2023

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“…Due to their nature and broad distribution, BCC bacteria have been proposed as objectionable organisms in warning letters, safety alerts, or recalls by the United States Food and Drug Administration. Global recalls related to BCC bacteria contamination were found frequently in water-based products, especially in pharmaceutical products, personal care products, and disinfectants ( Kuhn et al, 1982 ; Balkhy et al, 2005 ; De Volder and Teves, 2020 ; Tavares et al, 2020 ; Zou et al, 2020 ; Du et al, 2021 ). BCC isolates, especially B. cepacia , were identified in 22% of those recalls as the leading cause of microorganisms in non-sterile product contamination from 2004 to 2011 ( Jimenez, 2007 ; Sutton and Jimenez, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Specific biomarker mining and rapid detection of Burkholderia cepacia complex by recombinase polymerase amplification

Fan,

Wang,

Song

et al. 2023

Front. Microbiol.

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ObjectiveTo mine specific proteins and their protein-coding genes as suitable molecular biomarkers for the Burkholderia cepacia Complex (BCC) bacteria detection based on mega analysis of microbial proteomic and genomic data comparisons and to develop a real-time recombinase polymerase amplification (rt-RPA) assay for rapid isothermal screening for pharmaceutical and personal care products.MethodsWe constructed an automatic screening framework based on Python to compare the microbial proteomes of 78 BCC strains and 263 non-BCC strains to identify BCC-specific protein sequences. In addition, the specific protein-coding gene and its core DNA sequence were validated in silico with a self-built genome database containing 158 thousand bacteria. The appropriate methodology for BCC detection using rt-RPA was evaluated by 58 strains in pure culture and 33 batches of artificially contaminated pharmaceutical and personal care products.ResultsWe identified the protein SecY and its protein-coding gene secY through the automatic comparison framework. The virtual evaluation of the conserved region of the secY gene showed more than 99.8% specificity from the genome database, and it can distinguish all known BCC species from other bacteria by phylogenetic analysis. Furthermore, the detection limit of the rt-RPA assay targeting the secY gene was 5.6 × 102 CFU of BCC bacteria in pure culture or 1.2 pg of BCC bacteria genomic DNA within 30 min. It was validated to detect <1 CFU/portion of BCC bacteria from artificially contaminated samples after a pre-enrichment process. The relative trueness and sensitivity of the rt-RPA assay were 100% in practice compared to the reference methods.ConclusionThe automatic comparison framework for molecular biomarker mining is straightforward, universal, applicable, and efficient. Based on recognizing the BCC-specific protein SecY and its gene, we successfully established the rt-RPA assay for rapid detection in pharmaceutical and personal care products.

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“…In view of the significant morbidity and mortality associated with BCC infection, rapid and accurate detection is vital to initiate timely and effective treatment. Currently, BCC identification mainly involves isolation on selective media and DNA-based detection methods such as PCR and qPCR ( De Volder et al., 2021 ). However, novel methods that offer high sensitivity and specificity, while being quick and easy to perform, are much needed ( Ahn et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification

Gan

Tian

et al. 2022

Front. Cell. Infect. Microbiol.

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The Burkholderia cepacia complex (BCC) is a group of opportunistic pathogens, including Burkholderia cepacia, Burkholderia multivorans, Burkholderia vietnamiensis and Burkholderia ambifaria, which can cause severe respiratory tract infections and lead to high mortality rates among humans. The early diagnosis and effective treatment of BCC infection are therefore crucial. In this study, a novel and rapid recombinase-aided amplification (RAA) assay targeting the 16S rRNA gene was developed for BCC detection. The protocol for this RAA assay could be completed in 10 min at 39°C, with a sensitivity of 10 copies per reaction and no cross-reactivity with other pathogens. To characterize the effectiveness of the RAA assay, we further collected 269 clinical samples from patients with bacterial pneumonia. The sensitivity and specificity of the RAA assay were 100% and 98.5%, respectively. Seven BCC-infected patients were detected using the RAA assay, and three BCC strains were isolated from the 269 clinical samples. Our data showed that the prevalence of BCC infection was 2.60%, which is higher than the 1.40% reported in previous studies, suggesting that high sensitivity is vital to BCC detection. We also screened a patient with B. vietnamiensis infection using the RAA assay in clinic, allowing for appropriate treatment to be initiated rapidly. Together, these data indicate that the RAA assay targeting the 16S rRNA gene can be applied for the early and rapid detection of BCC pathogens in patients with an uncharacterized infection who are immunocompromised or have underlying diseases, thereby providing guidance for effective treatment.

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Distribution of Burkholderia cepacia complex species isolated from industrial processes and contaminated products in Argentina

Cited by 10 publications

References 44 publications

Burkholderia contaminans Bacteriophage CSP3 Requires O-Antigen Polysaccharides for Infection

Burkholderia contaminans Bacteriophage CSP3 Requires O-Antigen Polysaccharides for Infection

Specific biomarker mining and rapid detection of Burkholderia cepacia complex by recombinase polymerase amplification

Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification

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