Distribution of Cd, Zn and Cu in liver and gills of the eel Anguilla anguilla with special reference to metallothioneins

Noël-Lambot, F.; Gerday, Ch.; Distèche, A.

doi:10.1016/0306-4492(78)90130-2

Cited by 58 publications

(39 citation statements)

References 41 publications

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“…We have no information on the raltive binding affinities for the three metals in bluegills. Noel-Lambot et al found that zinc, and not copper, could be displaced by cadmium on the MTs in laiver of eels (23). Our data indicate that Cu may Limited data are available on the changes that occur with time in the quantities of metals bound to MTs even though in many ecosystems fish are subjected to chronic exposure to low levels of metals.…”

Section: Discussionmentioning

confidence: 44%

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Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Harrison¹,

Lam²

1986

Environmental Health Perspectives

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Livers from bluegills exposed to increased soluble copper (Cu) under field and laboratory conditions were analyzed to determine the concentration and distribution of Cu in metalloproteins of different molecular size. Analyses were performed on bluegills collected from the impoundment of the H. B. Robinson Steam Electric Plant (Florence, SC) near the effluent discharge from the power plant, near the water intake to the cooling system, and from a control pond as well as on bluegills exposed under controlled laboratory conditions. Metalloproteins were separated into low molecular weight (LMW), intermediate molecular weight (IMW), and high molecular weight (HMW) fractions by using high-performance liquid chromatography.In the field-exposed bluegills, Cu concentrations in the LMW, IMW, and HMW fractions were highest in bluegills from the discharge site and lowest in those from the control pond. In the laboratory-exposed bluegills, Cu concentrations in the fractions increased with exposure concentration and time. Concentrations of Cu in the LMW protein fraction and pellet of bluegills exposed to 160 jig Cu/L appeared to plateau with long exposure times, whereas those in the HMW fraction continued to increase. Bluegills maintained in 80 ,ug Cu/L water at pH 5.5 accumulated lower concentrations of Cu in the LMW and pellet fractions and higher amounts in the HMW than in those maintained in 80 jig Cu/L at pH 7.0. Mortality was dependent on exposure concentration and duration and was higher in bluegills maintained in water at pH 5.5 than at pH 7.0.

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Section: Discussionmentioning

confidence: 44%

“…Experiments to determine the effect of copper exposure on MTs in fish have been perforned only on coho salmon (20)(21)(22) and rainbow trout (30)(31)(32). However, copper binding to MTs has been detected in the eel (23), flounder (9), chum salmon (10), killifish (27), skipjack (33), yellowtail (33), blackbream (33), and carp (33).…”

Section: Introductionmentioning

confidence: 99%

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Harrison¹,

Lam²

1986

Environmental Health Perspectives

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“…The liver plays an important role in accumulation and detoxification of HMs [16]. Exposure of fish to elevated levels of HMs induces the synthesis of metallothioneine proteins (MT), which are metal binding proteins [17,18]. Fishes are known to possess the metallothioneine proteins [19].…”

Section: Comparison In the Concentrations Of Hms In Different Organs mentioning

confidence: 99%

Heavy Metals (Lead, Cadmium and Nickel) Concentration in Different Organs of Three Commonly Consumed Fishes in Bangladesh

Mnh¹,

Bc²,

Rahman³

2017

Fish Aquac J

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Pollution and food safety are concerning issues in recent years. For that, the present investigation was aimed to determine the levels of heavy metals (Lead, Cadmium and Nickel) in different organs of three fish (Labeo rohita, Gibelion catla and Pangasius hypophthalmus) and to compare the present value with Food and Agriculture Organization (FAO)/World Health Oraganization (WHO) acceptable limit. These commonly consumed fish were collected from Kawran Bazar fish market, Bangladesh and the concentrations of heavy metals were carried out using Atomic Absorption Spectrophotometer. The highest (48.33 ± 2.52 ppm) concentration of Lead (Pb) was found in the gill of Pangus fish and the lowest (23.33 ± 2.52 ppm) was found in the gonad of Rui fish. In case of Cadmium (Cd), the highest (0.96 ± 0.08 ppm) concentration was found in the gill of Rui fish whereas the lowest (0.02 ± 0.01 ppm) was found in the kidney of Pangus fish. The highest (6.63 ± 1.00 ppm) concentration of Nickel (Ni) was found in the gill of Katla fish and the lowest (0.10 ± 0.10 ppm) was found in the liver of the same species. The metal concentrations in the muscle of Rui, Katla and Pangus fish were in the decreasing order of Pb>Ni>Cd, Pb>Cd>Ni and Pb>Ni>Cd respectively. In the study, it was found that Pb has exceeded the limits in every case whereas Cd was within the limits except for a few and Ni was completely within the maximum allowable limits provided by FAO/ WHO. However, all these concentrated metals in different parts of fish body could be concentrated into human body, if they are consumed and if so happen; there will be a massive health risk for us.

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“…Indeed recent work (Millar et al 1989) has shown the induction of P450E (CYP 1Al) in the pillar cells of the gill by immunoblotting techniques following intraperitoneal injection of the model inducer P-napthoflavone (BNF). Several studies have shown the induction of metal binding proteins in the gill in response to waterborne zinc (Kito et al 1982, Bradley et al 1985, cadmium (Noel-Lambot et al 1978, Reichert et al 1979, Kito et al 1982, Thomas et al 1983, 1985, Benson & Birdge 1985, Klaverkamp & Duncan 1987, Olsson & Hogstrand 1987, Olsson et al 1989) and mercury (Bouquegneau et al 1975). Induction of metallothionein genes has been shown in gills in response to intraperitoneal injection of cadmium, copper, and zinc (Zafarullah et al 1989) although in response to lower Cd doses (Norey et al 1990a, b) induced gene expression, but no increase in metallothionein, could be detected.…”

Section: Introductionmentioning

confidence: 99%

Changes in branchial Na+,K+-ATPase, metallo-thionein and P450 1A1 in dab Limanda limanda in the German Bight: indicators of sediment contamination?

Stagg¹,

Goksøyr²,

Rodger³

1992

Mar. Ecol. Prog. Ser.

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Na+,K+-ATPase activity, ouabain binding, metallothionein and P450 1Al were measured in gill tissue from the dab Lirnanda limanda caught from a contaminant gradient in the German Bight during the Bremerhaven Workshop. Metallothionein levels were highest at the most contaminated sites closest to the mouth of the Elbe and lowest at the outermost station on the Dogger Bank. Tissue levels of zinc and cadmium were also elevated at these sites but copper levels did not change along the gradient. P450 1Al levels, measured with an antibody probe, showed a complex pattern of induction which suggests an inhibitory effect at the most contaminated stations. Na+,Kc-ATPase was similar in fish from all of the stations but measurement of ouabain binding show that levels of the enzyme are elevated at the innermost and outer stations. Calculation of activity per binding site indicates that the turnover rates of the enzyme were lower at the most contaminated stations. The results show that the role of adaptive responses in the face of inhibition by contaminants is an important consideration in identifying biological effects. The data were analysed with respect to the contaminant burdens in the total and fine particulate fraction (< 63 pm) of the sediment and in the liver of dab caught at each station. The utility of biological effects measurement in the gill as an indicator of environmental contamination is discussed.

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Distribution of Cd, Zn and Cu in liver and gills of the eel Anguilla anguilla with special reference to metallothioneins

Cited by 58 publications

References 41 publications

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Heavy Metals (Lead, Cadmium and Nickel) Concentration in Different Organs of Three Commonly Consumed Fishes in Bangladesh

Changes in branchial Na+,K+-ATPase, metallo-thionein and P450 1A1 in dab Limanda limanda in the German Bight: indicators of sediment contamination?

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