Distribution of cells expressing Jun and Fos proteins and synthesizing DNA in the adrenal cortex of hypophysectomized rats: regulation by ACTH and FGF2

Torres, Thompson E.P.; Lotfi, Claudimara Ferini Pacicco

doi:10.1007/s00441-007-0436-0

Cited by 10 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous findings from our laboratory have shown that ACTH administration in hypophysectomized (Hyp) or Dextreated rats induces S-phase entry in all zones of the adrenal cortex, which confirms that the adrenocortical proliferative capacity is not restricted to the outer adrenal zone (Baccaro et al 2007;Torres and Lotfi 2007). Moreover, these studies have implicated the AP-1 family of transcription factors in the regulation of the cell cycle during ACTH-induced proliferation in the adrenal cortex .…”

Section: Introductionsupporting

confidence: 71%

“…Conversely, evidence is also available that each zone can be renewed from within itself. Although proliferation is more frequent in the subcapsular/glomerulosa cell zone, it is not restricted to this region, since cell proliferation also occurs in the ZF and ZR (McEwan et al 1999;Baccaro et al 2007;Torres and Lotfi 2007). Additionally, there is evidence that POMC-derived peptides other than ACTH play a role in the control of adrenal growth.…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Synthetic modified N-POMC1–28 controls in vivo proliferation and blocks apoptosis in rat adrenal cortex

2010

View full text Add to dashboard Cite

The identity of the pro-opiomelanocortin (POMC)-derived mitogen in the adrenal cortex has been historically controversial. We have used well-established in vivo models, viz., hypophysectomized (Hyp) or dexamethasone (Dex)-treated rats, to study the effect of the synthetic modified peptide N-terminal POMC (N-POMC(1-28)) on DNA synthesis in the adrenal cortex, as assessed by BrdU incorporation and compared with adrenocorticotropic hormone (ACTH). We evaluated the importance of disulfide bridges on proliferation by employing N-POMC(1-28) without disulfide bridges and with methionines replacing cysteines. Acute administration of synthetic modified N-POMC(1-28) distinctly increased DNA synthesis in the zona glomerulosa and zona fasciculata, but not in the zona reticularis in Hyp rats, whereas in Dex-treated rats, this peptide was effective in all adrenal zones. ACTH administration led to an increase of BrdU-positive cells in all adrenal zones irrespective of the depletion of Hyp or Dex-POMC peptides. The use of the ACTH antagonist, ACTH(7-38), confirmed the direct participation of ACTH in proliferation. Two different approaches to measure apoptosis revealed that both peptides similarly exerted a protective effect on all adrenocortical zones, blocking the apoptotic cell death induced by hypophysectomy. Thus, ACTH(1-39) and N-POMC(1-28) have similar actions suggesting that the disulfide bridges are important but not essential. Both peptides seem to be important factors determining adrenocortical cell survival throughout the adrenal cortex, reinforcing the idea that each zone can be renewed from within itself.

show abstract

Section: Introductionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 89%

Synthetic modified N-POMC1–28 controls in vivo proliferation and blocks apoptosis in rat adrenal cortex

2010

View full text Add to dashboard Cite

show abstract

“…Products encoded by these genes regulate transition of mouse Y1 and H295R cells from G 0 /G 1 to S phase of the cell cycle (43,49). Similar mechanisms control proliferative activity of rat adrenocortical cells in vivo (50). Our study revealed that ACTH, CER and desCER have a potent stimulatory effect on expression of the Fos gene in cultured rat adrenocortical cells, however, expression of Jun gene was unaffected.…”

Section: Discussionmentioning

confidence: 51%

Cerebellin and des-cerebellin exert ACTH-like effects on corticosterone secretion and the intracellular signaling pathway gene expression in cultured rat adrenocortical cells - DNA microarray and QPCR studies

Ruciński

Ziółkowska²,

Szyszka³

et al. 2009

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Precerebellins (Cbln) belong to the C1q/TNF superfamily of secreted proteins which have diverse functions. They are abundantly expressed in the cerebellum, however, three of them are also expressed in the rat adrenal gland. All members of the Cbln family form homomeric and heteromeric complexes with each other in vitro and it was suggested that such complexes play a crucial role in normal development of the cerebellum. The aim of our study was to investigate whether Cbln1-derived peptides, cerebellin (CER) and des-Ser 1 -cerebellin (desCER) are involved in regulating biological functions of rat adrenocortical cells. In the primary culture of rat adrenocortical cells, 24 h exposure to CER or desCER notably stimulated corticosterone output and inhibited proliferative activity and similar effects were evoked by ACTH. To study gene transcript regulation by CER, desCER and ACTH, we applied Oligo GEArray ® DNA Microarray: Rat Signal Transduction Pathway Finder™. In relation to the control culture, 13 of the 113 transcripts present on the array were differentially expressed. These transcripts were either up-or down-regulated by ACTH and/or CER or desCER treatment. Validation of DNA Microarray data by QPCR revealed that only 5 of 13 genes studied were differentially expressed. Of those genes, Fos and Icam1 were up-regulated and Egr1 was down-regulated by ACTH, CER and desCER. The remaining two genes, Fasn (insulin signaling pathway) and Hspb1 (HSP27) (stress signaling pathway), were regulated only by CER and desCER, but not by ACTH. Thus, both CER and desCER have effects similar to and different from corticotrophin on the intracellular signaling pathway gene expression in cultured rat adrenocortical cells.

show abstract

“…Jun and Fos protooncogenes were identified as important transcription factors activated very early (10 minutes) after partial hepatectomy [19]. In adrenocortical cells Fos gene is induced by both ACTH and Fgf2 [58–60]. In our experiments in the beginning of regeneration (days 1-2) only Fos gene has been found to be upregulated, while between days 5 and 15 after enucleation a lowered expression of both genes has been found.…”

Section: Discussionmentioning

confidence: 59%

Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation

Tyczewska

Ruciński

Ziółkowska

et al. 2014

International Journal of Endocrinology

View full text Add to dashboard Cite

Enucleation-induced adrenal regeneration is a highly controlled process; however, only some elements involved in this process have been recognized. Therefore, we performed studies on regenerating rat adrenals. Microarray RNA analysis and QPCR revealed that enucleation resulted in a rapid elevation of expression of genes involved in response to wounding, defense response, and in immunological processes. Factors encoded by these genes obscure possible priming effects of various cytokines on initiation of regeneration. In regenerating adrenals we identified over 100 up- or downregulated genes involved in adrenocortical cell proliferation. The changes were most significant at days 2-3 after enucleation and their number decreased during regeneration. For example, expression analysis revealed a notable upregulation of the growth arrest gene, Gadd45, only 24 hours after surgery while expression of cyclin B1 and Cdk1 genes was notably elevated between days 1–8 of regeneration. These changes were accompanied by changes in expression levels of numerous growth factors and immediate-early transcription factors genes. Despite notable differences in mechanisms of adrenal and liver regeneration, in regenerating adrenals we identified genes, the expression of which is well recognized in regenerating liver. Thus, it seems legitimate to suggest that, in the rat, the general model of liver and adrenal regeneration demonstrate some degree of similarity.

show abstract

Distribution of cells expressing Jun and Fos proteins and synthesizing DNA in the adrenal cortex of hypophysectomized rats: regulation by ACTH and FGF2

Cited by 10 publications

References 38 publications

Synthetic modified N-POMC1–28 controls in vivo proliferation and blocks apoptosis in rat adrenal cortex

Synthetic modified N-POMC1–28 controls in vivo proliferation and blocks apoptosis in rat adrenal cortex

Cerebellin and des-cerebellin exert ACTH-like effects on corticosterone secretion and the intracellular signaling pathway gene expression in cultured rat adrenocortical cells - DNA microarray and QPCR studies

Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation

Contact Info

Product

Resources

About