Distribution of cholecystokinin‐like‐immunoreactive neurons in the guinea pig forebrain

Ciofi, Philippe; Tramu, G.

doi:10.1002/cne.903000107

Cited by 24 publications

(20 citation statements)

References 162 publications

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“…The specificity controls performed by immunocytochemistry and radioimmunoassay of the cholecystokinin (CCK) antiserum IS-15/8 show the expected crossreaction with gastrin (Ciofi and Tramu, 1990). CCK labeling is mainly found in large somata at the EPL/GL border (presumably in medium-sized tufted cells), as also reported using a mouse monoclonal antibody Kosaka and Kosaka, 2007b).…”

Section: Antibodies Characterizationsupporting

confidence: 65%

Somatostatin interneurons delineate the inner part of the external plexiform layer in the mouse main olfactory bulb

Lepousez

Csaba

Bernard

et al. 2010

J of Comparative Neurology

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Neuropeptides play a major role in the modulation of information processing in neural networks. Somatostatin, one of the most concentrated neuropeptides in the brain, is found in many sensory systems including the olfactory pathway. However, its cellular distribution in the mouse main olfactory bulb (MOB) is yet to be characterized. Here we show that approximately 95% of mouse bulbar somatostatin-immunoreactive (SRIF-ir) cells describe a homogeneous population of interneurons. These are restricted to the inner lamina of the external plexiform layer (iEPL) with dendritic field strictly confined to the region. iEPL SRIF-ir neurons share some morphological features of Van Gehuchten short-axon cells, and always express glutamic acid decarboxylase, calretinin, and vasoactive intestinal peptide. One-half of SRIF-ir neurons are parvalbumin-ir, revealing an atypical neurochemical profile when compared to SRIF-ir interneurons of other forebrain regions such as cortex or hippocampus. Somatostatin is also present in fibers and in a few sparse presumptive deep short-axon cells in the granule cell layer (GCL), which were previously reported in other mammalian species. The spatial distribution of somatostatin interneurons in the MOB iEPL clearly outlines the region where lateral dendrites of mitral cells interact with GCL inhibitory interneurons through dendrodendritic reciprocal synapses. Symmetrical and asymmetrical synaptic contacts occur between SRIF-ir dendrites and mitral cell dendrites. Such restricted localization of somatostatin interneurons and connectivity in the bulbar synaptic network strongly suggest that the peptide plays a functional role in the modulation of olfactory processing.

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Section: Antibodies Characterizationsupporting

confidence: 65%

Somatostatin interneurons delineate the inner part of the external plexiform layer in the mouse main olfactory bulb

Lepousez

Csaba

Bernard

et al. 2010

J of Comparative Neurology

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“…Labeling is abolished in the rodent brain by preadsorption with porcine galanin but not by porcine neuropeptide Y (NPY), GnRH, or vasopressin (Gasman et al, 1996;Ciofi and Tramu, unpublished observations Characterization by radioimmunoassay showed cross-reactivity with CCK-33 and gastrin but no cross-reactivity with substance P, Leu-or Met-enkephalin, b-endorphin, or FMRFamide (Studler et al, 1984). Immunocytochemical labeling is abolished in rodent brain by preadsorption with 10 À6 M CCK-8 and gastrin 1, but not by neurotensin, human growth hormone-releasing hormone (GHRH), porcine NPY, substance P, or leu-or Met-enkephalin (Ciofi and Tramu, 1990). The distribution of CCK-immunoreactive cell bodies reported here matches the mapping of CCK mRNA expression in mouse brain (Allen Institute Brain Atlas).…”

Section: Antibody Characterizationmentioning

confidence: 89%

Kisspeptin neurons co‐express met‐enkephalin and galanin in the rostral periventricular region of the female mouse hypothalamus

Porteous

Petersen

Yeo

et al. 2011

J of Comparative Neurology

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It is now well established that the kisspeptin neurons of the hypothalamus play a key role in regulating the activity of gonadotropin-releasing hormone (GnRH) neurons. The population of kisspeptin neurons residing in the rostral periventricular region of the third ventricle (RP3V), encompassing the anteroventral periventricular (AVPV) and periventricular preoptic nuclei (PVpo), are implicated in the generation of the preovulatory GnRH surge mechanism and puberty onset in female rodents. The present study examined whether these kisspeptin neurons may express other neuropeptides in the adult female mouse. Initially, the distribution of galanin, neurotensin, met-enkephalin (mENK), and cholecystokinin (CCK)-immunoreactive cells was determined within the RP3V of colchicine-treated mice. Subsequent experiments, using a new kisspeptin-10 antibody raised in sheep, examined the relationship of these neuropeptides to kisspeptin neurons. No evidence was found for expression of neurotensin or CCK by RP3V kisspeptin neurons, but subpopulations of kisspeptin neurons were observed to express galanin and mENK. Dual-labeled RP3V kisspeptin/galanin cells represented 7% of all kisspeptin and 21% of all galanin neurons whereas dual-labeled kisspeptin/mENK cells represented 28-38% of kisspeptin neurons and 58-68% of the mENK population, depending on location within the AVPV or PVpo. Kisspeptin neurons in the arcuate nucleus were also found to express galanin but not mENK. These observations indicate that, like the kisspeptin population of the arcuate nucleus, kisspeptin neurons in the RP3V also co-express a range of neuropeptides. This pattern of co-expression should greatly increase the dynamic range with which kisspeptin neurons can modulate the activity of their afferent neurons.

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“…The following antisera were used: anti-KP (#AC024, 1:5000, from A. Caraty, INRA, Nouzilly, France), anti-galanin (# IS-42; 1:2000, Interchim Cat# AP101500, RRID: AB_2314518), anti-CCK-8 (# IS-15/8 RRID: AB_2314186, 1:1000, from P. Ciofi, National Institute of Health and Medical Research, Bordeaux, France) and anti-tyrosine hydroxylase (TH) (#TYH, 1:1000; Aves Labs Inc., Tigard, OR, USA). The characterization and specificity of the primary antibodies have been published elsewhere (Ciofi and Tramu, 1990; Porteous et al, 2011), the pre-absorption of the antibodies or their omission from the staining resulted in immunonegative sections. Cy3-conjugated secondary antibodies (Jackson Immunoresearch Europe Ltd, Suffolk, UK) were diluted in PBS containing 2% NHS (1:500) and applied on the sections for 4 h at RT.…”

Section: Methodsmentioning

confidence: 99%

Impact of Proestrus on Gene Expression in the Medial Preoptic Area of Mice

Vastagh

Liposits

2017

Front. Cell. Neurosci.

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The antero-ventral periventricular zone (AVPV) and medial preoptic area (MPOA) have been recognized as gonadal hormone receptive regions of the rodent brain that—via wiring to gonadotropin-releasing hormone (GnRH) neurons—contribute to orchestration of the preovulatory GnRH surge. We hypothesized that neural genes regulating the induction of GnRH surge show altered expression in proestrus. Therefore, we compared the expression of 48 genes obtained from intact proestrous and metestrous mice, respectively, by quantitative real-time PCR (qPCR) method. Differential expression of 24 genes reached significance (p < 0.05). Genes upregulated in proestrus encoded neuropeptides (kisspeptin (KP), galanin (GAL), neurotensin (NT), cholecystokinin (CCK)), hormone receptors (growth hormone secretagogue receptor, μ-opioid receptor), gonadal steroid receptors (estrogen receptor alpha (ERα), progesterone receptor (PR), androgen receptor (AR)), solute carrier family proteins (vesicular glutamate transporter 2, vesicular monoamine transporter 2), proteins of transmitter synthesis (tyrosine hydroxylase (TH)) and transmitter receptor subunit (AMPA4), and other proteins (uncoupling protein 2, nuclear receptor related 1 protein). Proestrus evoked a marked downregulation of genes coding for adenosine A2a receptor, vesicular gamma-aminobutyric acid (GABA) transporter, 4-aminobutyrate aminotransferase, tachykinin precursor 1, NT receptor 3, arginine vasopressin receptor 1A, cannabinoid receptor 1, ephrin receptor A3 and aldehyde dehydrogenase 1 family, member L1. Immunocytochemistry was used to visualize the proteins encoded by Kiss1, Gal, Cck and Th genes in neuronal subsets of the AVPV/MPOA of the proestrous mice. The results indicate that gene expression of the AVPV/MPOA is significantly modified at late proestrus including genes that code for neuropeptides, gonadal steroid hormone receptors and synaptic vesicle transporters. These events support cellular and neuronal network requirements of the positive estradiol feedback action and contribute to preparation of the GnRH neuron system for the pre-ovulatory surge release.

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Distribution of cholecystokinin‐like‐immunoreactive neurons in the guinea pig forebrain

Cited by 24 publications

References 162 publications

Somatostatin interneurons delineate the inner part of the external plexiform layer in the mouse main olfactory bulb

Somatostatin interneurons delineate the inner part of the external plexiform layer in the mouse main olfactory bulb

Kisspeptin neurons co‐express met‐enkephalin and galanin in the rostral periventricular region of the female mouse hypothalamus

Impact of Proestrus on Gene Expression in the Medial Preoptic Area of Mice

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