Distribution of Class 1 and 2 Integrons Among Salmonella Enterica Serovars Isolated from Iranian Patients

Ranjbar, Reza; Taghipour, Fatemeh; Afshar, Davoud; Farshad, Shohreh

doi:10.2174/1874285801913010063

Cited by 2 publications

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References 22 publications

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“…Integron-inserted gene cassettes have been reported in Aeromonas species isolated from fish in different countries, including China [6,7], Thailand [8], USA [9], Australia [10], Mexico [11], and South Africa [12][13][14]. However, the prevalence of the class 1 integron in Iran's aquaculture industry has not been addressed, and most studies in this area have focused on the prevalence of class 1 and 2 integrons in clinical isolates and environmental samples [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance and Incidence of Integrons in Aeromonas Species Isolated from Diseased Freshwater Animals and Water Samples in Iran

Ranjbar

Sharifiyazdi

2019

Antibiotics

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Aeromonas spp. is one of the major pathogens of freshwater animals. There has been little research on the genetics of antimicrobial resistance associated with it in Iranian aquaculture. To remedy this lack in research, 74 multi-drug-resistant Aeromonas spp. were isolated from farmed diseased carp, trout, sturgeon, ornamental fish, crayfish, and corresponding water samples and examined for genomic integron sequences. Class 1 integrons, containing seven types of integron cassette arrays (dfrA1-aadA1, dfrA1-orfC, dfrA12-aadA2, dfrA12-orfF-aadA2, dfrA15, dfrB4-catB3-aadA1, aac(6’)-Ib-cr-arr3-dfrA27) were found in 15% of the resistant isolates; no class 2 integrons were detected in any of the resistant isolates. As some tested isolates were resistant to more than two groups of antibiotics, our results demonstrated that freshwater animals in Iran could be a source of multiply drug-resistant Aeromonas spp. This finding suggests that the origin of the antimicrobial resistance of these animals be placed under increased surveillance in the future and that the use of antimicrobials be limited in aquaculture.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance and Incidence of Integrons in Aeromonas Species Isolated from Diseased Freshwater Animals and Water Samples in Iran

Ranjbar

Sharifiyazdi

2019

Antibiotics

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Molecular Study of Integrase Gene I and Integrase Gene II in Clinical Isolates of Pseudomonas aeruginosa

Zaki

Mahmoud

Rizk

2022

IDDT

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Background: The presence of class I integron gene is associated with the emergence of multiple drug resistance [MDR] phenotype in Pseudomonas aeruginosa [P. aeruginosa] isolates. Aim: The objectives were to study the prevalence of integrase genes I and II [Intel I & Intel II] in clinical isolates of P. aeruginosa and its association with antibiotics resistance in these isolates. Methods: The study was a retrograde cross-sectional study that was carried out on 150 clinical isolates of P. aeruginosa isolated from patients with health care associated infections. The isolates were subjected to biochemical identification and antibiotics sensitivity study by discs diffusion test. Intel I & Intel II genes were detected by polymmulerase chain reaction [PCR]. Results: Intel I gene was present in 48% of the isolates and Intel II was present in 1.3% of the isolates. Intel I gene was detected in statistically significant high rate in MDR- P. aeruginosa [76.9%, P=0.001] compared to non MDR- P. aeruginosa [3.4%]. While, intel II had statistically insignificant increase in MDR- P. aeruginosa [1.1%, P=1.00] compared to non MDR-P. aeruginosa [1.7%]. Both Intl I/ Intl II genes were detected together in 2.2% of MDR- P. aeruginosa isolates and were absent in non MDR-P. aeruginosa isolates with statistically insignificant difference [P=1.00]. P. aeruginosa isolates with Intel I gene had increase in antibiotics resistance pattern to the used antibiotics discs, However, this increase had statistically significance rates only to gentamicin [63.9%, P≤0.001], meropenem [47.2%, P=0.009], trimethoprim/sulfamethoxazole [37.5%, P=0.013] and imipenem [44.4%, P=0.025]. Conclusion: The present study highlights the high prevalence of Intel I gene in clinical isolates of P. aeruginosa, while Intel II gene was less prevalent in these isolates. There was significant association between the prevalence of Intel I gene and MDR phenotype of P. aeruginosa and resistance to gentamicin, meropenem, trimethoprim/sulfamethoxazole and imipenem. These findings need future evaluation in higher number of clinical isolates of P. aeruginosa.

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Distribution of Class 1 and 2 Integrons Among Salmonella Enterica Serovars Isolated from Iranian Patients

Cited by 2 publications

References 22 publications

Antimicrobial Resistance and Incidence of Integrons in Aeromonas Species Isolated from Diseased Freshwater Animals and Water Samples in Iran

Antimicrobial Resistance and Incidence of Integrons in Aeromonas Species Isolated from Diseased Freshwater Animals and Water Samples in Iran

Molecular Study of Integrase Gene I and Integrase Gene II in Clinical Isolates of Pseudomonas aeruginosa

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