Distribution of Collagen in the Lamina Propria of the Human Vocal Fold

Melo, Erich Christiano Madruga de; Lemos, Miriam; Filho, João Aragão Ximenes; Sennes, Luiz Ubirajara; Saldiva, Paulo Hilário Nascimento; Tsuji, Domingos Hiroshi

doi:10.1097/00005537-200312000-00027

Cited by 95 publications

(75 citation statements)

References 13 publications

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“…The picrosirius stain showed abundant type I collagen but little in the way of Type III collagen within the elderly LP. This finding is supported by Melo et al (2003) who reported that in older vocal folds the proportion of Type I collagen is increased compared with Type III. Wang et al (2008) notes that Type III collagen becomes abundant compared with Type I collagen during tissue repair and proliferation, perhaps suggesting that the regenerative capacity of the fibroblasts within the VF LP is somewhat constrained in the elderly.…”

Section: Discussionsupporting

confidence: 68%

Microstructure of the vocal fold in elderly humans

2011

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Significant changes in the voice occur after the age of 50 years. Changes in the structure of the vocal fold (VF) can interfere with the voice. The aim of this study is to investigate the structure of the VF of elderly people that may contribute to the tendency of the human voice to deteriorate. Larynges were obtained from eight embalmed cadavers aged 72-98 years. The middle portion of each vocal fold was removed and placed in 4% buffered formalin. Tissue blocks were then processed and embedded in wax. Four to six micron coronal sections were cut and stained with Haematoxylin and Eosin (H&E), Masson's Trichrome (MTS), Elastin van Gieson (EVG), Herovici (HERO), picrosirius and CD31 immunohistochemical marker in order to study the collagen fibers, elastic fibers and microvasculature of the VF. The maturity of collagen fibers within the VF were noted to increase from the superficial to the deep layer of the lamina propria (LP). Contrary to current literature, the amount of elastic fibers was sparse in the superficial layer of the LP in the vocal tissue of elderly cadavers. Numerous cross-sectioned blood vessels were seen in the lamina propria near the free edge, and near the superior and inferior surfaces of the VF. The presence of lymphatic vessels was confirmed in the VF of elderly subjects. This study revealed that the collagenous component of the deep layer of the VF LP was made up of mature fibers whilst immature collagen fibers made up the superficial layer of the LP. There was a notable scarcity of elastic fibers in the superficial layer of the LP. Lymphatics were seen and were orientated differently in the geriatric vocal folds.

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Section: Discussionsupporting

confidence: 68%

Microstructure of the vocal fold in elderly humans

2011

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“…The accumulation of these proteins is of particular interest because they comprise the principle fibrous and interstitial proteins present within the human vocal fold [21][22][23]. The identification of strategies that accelerate the synthesis, accumulation and harvesting of these biomaterials may lead to the Fig.…”

Section: Discussionmentioning

confidence: 99%

The effect of bioreactor induced vibrational stimulation on extracellular matrix production from human derived fibroblasts

et al. 2009

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“…Most of the papers on the larynx have studied the mucosal layer that lines the vocal folds; various modifications have been described, such as: the reduced number of elastic fibers in the middle layer of the lamina propria; 1 the reduced density of epithelial cells and the thickness of the lamina propria; 2,3 the increased quantity of collagen in the lamina propria; 4 morphological changes in elastic fibers located in the superficial layer of the lamina propria; 5 and collagen fiber architectural derangement in the lamina propria. 6 Studies on the laryngeal muscle system have revealed results that suggest the following: a reduced number of muscle fibers in the thyroarytenoid muscle, particularly slow contraction muscle fibers, 7 and a reduced amount of those proteins that are responsible for muscle contraction. 8 Based on electromyography studies, other authors have presented results that suggest denervation or axonal injury involving laryngeal motor control in the elderly, leading to altered contraction of laryngeal muscles.…”

Section: Introductionmentioning

confidence: 99%