Distribution of Cryptosporidium parvum subgenotypes in pre-weaned calves in Germany

Göhring, F.; Lendner, Matthias; Daugschies, Arwid

doi:10.1016/j.vprsr.2022.100806

Cited by 2 publications

(1 citation statement)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(Chalmers et al, 2019). In the IIa subtype family of C. parvum, the rst 9 subtypes are IIaA15G2R1 (32.4%, 742/2293), France and the Netherlands, it was found to be 20.5% in Belgium and 6.4% in the Netherlands(Pinto et al, 2021) In the subtyping study conducted in weaned calves in Germany, IIaA13G2R1 subtype was found at a rate of 0.4% (1/226), while the second most common IIaA14G1R1 subtype was found at a rate of 3.1% (7/226)(Göhring et al,2022). In the studies conducted by Yıldırım et al in Burdur and Kayseri province, which were evaluated within the scope of the study, IIaA13G2R1 was detected in 88% (122/138) of calves and heifers.…”

mentioning

confidence: 98%

Determination of Cryptosporidium Parvum Subtypes Detected in Calf and Cow

AYNUR

2023

Preprint

View full text Add to dashboard Cite

Cryptosporidium spp., an intracellular extracytoplasmic localized protozoan, is one of the leading infectious agents in the etiology of neonatal diarrheal syndrome of ruminants. Although cryptosporidiosis is a common disease seen all over the world. However molecular epidemiologic studies are limited on distribution of Cryptosporidium parvum subtypes in the world. To date, nearly 20 subtypes of C. parvum have been described. Gene sequence analysis of the glycoprotein 60 structural protein (GP60) is used to identify subtypes. It is reported that the GP60 gene sequence shows a high degree of genetic polymorphism among the strains. In this study, it was aimed to determine the subtypes of C. parvum parasite in cow and calf stool samples by using GP60 gene sequence analysis. In our study, 125 samples evaluated as Cryptosporidium spp. positive by Kinyoun Acid Fast staining. A total of 16 samples of cows (> 22 months) and 109 calves (< 30 days) from farms in 2 regions of Türkiye (Burdur/Yesilova and Aydin provinces) were used. DNA isolation from feces was done with the QIAamp Stool Mini Kit. In order to identify Cryptosporidium species, PCR based on SSU 18S rRNA gene amplification was performed. The 18S gene sequences were compared to genebank to identify the specie of the infectious agent. Nested PCR was performed to amplify GP60 gene and sequences of amplicons were used to identify subtypes. The results of C. parvum subtyping study showed that IIaA13G2R1 subtype was found to be common. IIaA13G2R1 was detected in 100% of 29 stool samples with C. parvum in Burdur. The diversity was higher among Aydin samples (n=96), 67 (70%) were IIaA13G2R1, 15 (15,5%) IIaA14G1R1, 12(12,5%) IIaA2R1 and one sample (1%) was IIaA16R1 and one sample (1%) IIaA5G1R1. In subtype studies based on the GP60 sequence, IIaA15G2R1 was found to be the most common in the world. In our study, IIaA13G2R1 subtype detected in cattle in Türkiye was found to be common. Our study is the first Cryptosporidium parvum subtyping study in Aydın province. In our study, IIaA2R1 and IIaA5G1R1 subtypes were defined for the first time in the world, IIaA16R1 subtype in Türkiye. Molecular epidemiology studies are important for better understanding the dissemination of subtypes and IIaA13G2R1 is the most common subtype in Turkey and the IIaA15G2R1 the most common subtype in the world were absent among our samples.

show abstract

mentioning

confidence: 98%

Determination of Cryptosporidium Parvum Subtypes Detected in Calf and Cow

AYNUR

2023

Preprint

View full text Add to dashboard Cite

show abstract

Molecular Detection and Distribution of Giardia duodenalis and Cryptosporidium spp. Infections in Wild and Domestic Animals in Portugal

Figueiredo,

Köster,

Dashti

et al. 2023

Transboundary and Emerging Diseases

View full text Add to dashboard Cite

Enteric protozoan parasites Giardia duodenalis, Cryptosporidium spp., and, to a lesser extent, the ciliate Balantioides coli are responsible for severe human and animal intestinal disorders globally. However, limited information is available on the occurrence and epidemiology of these parasites in domestic, but especially wild species in Portugal. To fill this gap of knowledge, we have investigated G. duodenalis, Cryptosporidium spp., and B. coli occurrence, distribution, genetic diversity, and zoonotic potential by analyzing 756 fecal samples from several wild carnivores (n = 288), wild ungulates (n = 242), and domestic species (n = 226) collected across different areas of mainland Portugal. Overall, infection rates were 16.1% (122/756; 95% CI: 13.59–18.96) for G. duodenalis and 2.7% (20/756; CI: 1.62–4.06) for Cryptosporidium spp., while no ungulate sample analyzed yielded positive results for B. coli. Giardia duodenalis was found across a wide range of hosts and sampling areas, being most prevalent in the Iberian lynx (26.7%), the Iberian wolf (24.0%), and the domestic dog (23.9%). Cryptosporidium spp. was only identified in wild boar (8.4%), red fox (3.4%), Iberian lynx (3.3%), red deer (3.1%), and Iberian wolf (2.5%). Sequence analysis of G. duodenalis determined zoonotic assemblage A (subassemblage AI) in one roe deer sample, canine-specific assemblages C and D in Iberian wolf, red fox, and domestic dog, and ungulate-specific assemblage E in wild boar, sheep, cattle, and horse. Six Cryptosporidium species were identified: C. scrofarum in wild boar, C. canis in the Iberian wolf and red fox, C. ubiquitum in red deer and wild boar, C. felis in the Iberian lynx, and both C. ryanae and C. occultus in red deer. Giardia duodenalis and Cryptosporidium spp. coinfections were observed in 0.7% (5/756) of the samples. This is the first, most comprehensive, and largest molecular-based epidemiology study of its kind carried out in Portugal, covering a wide range of wild and domestic hosts and sampling areas. The detection of zoonotic Cryptosporidium spp. and G. duodenalis subassemblage AI demonstrates the role of wild and domestic host species in the transmission of these agents while representing a potential source of environmental contamination for other animals and humans.

show abstract

Distribution of Cryptosporidium parvum subgenotypes in pre-weaned calves in Germany

Cited by 2 publications

References 58 publications

Determination of Cryptosporidium Parvum Subtypes Detected in Calf and Cow

Determination of Cryptosporidium Parvum Subtypes Detected in Calf and Cow

Molecular Detection and Distribution of Giardia duodenalis and Cryptosporidium spp. Infections in Wild and Domestic Animals in Portugal

Contact Info

Product

Resources

About