Distribution of Culturable Phosphate-Solubilizing Bacteria in Soil Aggregates and Their Potential for Phosphorus Acquisition

He, Donglan; Wan, Wenjie

doi:10.1128/spectrum.00290-22

Cited by 20 publications

(9 citation statements)

References 58 publications

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“…By contrast, other study observed isolated Pseudomonas sp. strain PSB-2 exhibited good solubilization of TCP with a high SI (>4.0) (He and Wan, 2022). In the present study, MQR6 T was isolated from the rhizosphere soil of A. truncatum, and showed clear halos of solubilizing TCP with the largest SI value of 3.98.…”

Section: Discussionmentioning

confidence: 52%

“…Gluconic and 2-keto gluconic acids produced by bacteria play an important role in weathering and solubilization of phosphate in soil, acting as Ca 2+ chelators and providing the acidification of the external environment to dissolve the sparingly soluble calcium phosphates (Yu et al, 2011). Furthermore, the glucose dehydrogenase (gcd) gene, coding for the first enzyme in the direct oxidation pathway, contributes significantly to mineral phosphate solubilization by the plant growth-promoting rhizabacteria (Shariati et al, 2017;He and Wan, 2022). Similarly, our study revealed that the genes related to gluconic and 2-keto-Dgluconic acid production were found in the strain MQR6 T , including gcd, gdh, pqq, and bet (Table S3).…”

Section: Discussionmentioning

confidence: 99%

“…Genomic DNA was extracted using a Bacterial DNA Kit (Tiangen, Beijing, China) following the manufacturer's instructions. The 16S rRNA gene was amplified by PCR using the universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3') (He and Wan, 2022). and the purified PCR products were sequenced by Sangon Biotech (Shanghai, PR China).…”

Section: Phylogenetic 16s Rrna Gene Analysismentioning

confidence: 99%

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Isolation and characterization of phosphate-solubilizing bacterium Pantoea rhizosphaerae sp. nov. from Acer truncatum rhizosphere soil and its effect on Acer truncatum growth

Wang

et al. 2023

Front. Plant Sci.

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The Acer truncatum Bunge, widely distributed in North China, shows excellent tolerance to low-P soils. However, little information is available on potential phosphate-solubilizing bacterial (PSB) strains from the A. truncatum rhizosphere. The objectives of this work were to isolate and characterize PSB from A. truncatum rhizosphere soil and to evaluate the effect of inoculation with the selected strain on A. truncatum seedlings. The strains were characterized on the basis of phenotypic characteristics, carbon source utilization pattern, fatty acid methyl esters analysis, 16S rRNA gene and the whole-genome sequence. A Gram-negative and rod-shaped bacterium, designated MQR6T, showed a high capacity to solubilize phosphate and produce indole-3-acetic acid (IAA) and siderophores. The strain can solubilize tricalcium phosphate (TCP) and rock phosphate (RP), and the solubilization of TCP was about 60% more effective than RP. Phylogenetic analyses based on the 16S rRNA gene and whole-genome sequences revealed that strain MQR6T formed a distinct phyletic lineage as a new species within the genus Pantoea. The digital DNA-DNA hybridization value between strain MQR6T and the closely related strains was 19.5-23.3%. The major cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c), summed feature 8 (C18:1ω6c and/or C18:1ω7c), C14:0, C16:0, and C17:0 cyclo. Several genes related to IAA production, phosphonate transport, phosphate solubilization and siderophore biogenesis were found in the MQR6T genome. Furthermore, inoculation with the strain MQR6T significantly improved plant height, trunk diameter, dry weight and P accumulation in roots and shoot of A. truncatum seedlings compared to non-inoculated control. These plant parameters were improved even further in the treatment with both inoculation and P fertilization. Our results suggested that MQR6T represented a new species we named Pantoea rhizosphaerae, as a plant growth-promoting rhizobacterium that can solubilize inorganic P and improve growth of A. truncatum seedlings, emerging as a potential strategy to improve A. truncatum cultivation.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic 16s Rrna Gene Analysismentioning

confidence: 99%

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Isolation and characterization of phosphate-solubilizing bacterium Pantoea rhizosphaerae sp. nov. from Acer truncatum rhizosphere soil and its effect on Acer truncatum growth

Wang

et al. 2023

Front. Plant Sci.

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“…Our results confirmed the hypothesis that gcd community was obviously affected by land reclamation, and it was closely associated with labile and moderately labile legacy P fractions. From the practical viewpoint, the inoculation of effective gcd ‐harboring species as an environment friendly alternative to conventional chemical P fertilization is a promising P management strategy (He & Wan, 2022; Islam & Hossain, 2012).…”

Section: Discussionmentioning

confidence: 99%

Land reclamation increased labile and moderately labile P fractions and strengthened co‐occurrence network ofgcdcommunity in calcareous soils

2023

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Land reclamation significantly impacts the transformation and availability of phosphorus (P) in soils. P‐solubilizing microorganisms (PSMs) play key roles in activating soil P. However, the responses of PSMs to the buildup of P under land reclamation and the relationship between different P fractions and PSMs have not been clearly clarified. Here, an investigation was carried out containing three treatments: (i) vegetable field (VF), (ii) cropland (CF), and (iii) uncultivated land (UL). Different P fractions were measured; the abundance of stable oxygen isotope in phosphate of D.HCl‐Pi was determined. The diversity, composition, and co‐occurrence network of P‐solubilizing glucose dehydrogenase community (gcd) were analyzed. Compared with the UL treatment, soil total P in the treatments of VF and CF was significantly increased by 1.5 and 1.2 times, respectively. The proportions of labile and moderately labile P in the treatments of VF and CF were 151% and 58% higher than in the UL treatment. The α‐diversity of gcd community (i.e., Shannon and Chao1 indices) in the VF and CF treatments was significantly greater than in the UL treatment. The change in gcd community structure was closely associated with resin‐Pi, NaHCO3‐Pi, and NaOH‐Pi. The nodes and edges in the co‐occurrence networks of VF and CF treatments were higher than in the UL treatment. Overall, a substantial amount of P was accumulated after land reclamation, which was more pronounced by labile and moderately labile P than non‐labile P; the complexity and robustness of gcd community's co‐occurrence network were notably strengthened by land reclamation.

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“…Soil carbon storage, nutrient availability, and biodiversity maintenance are strongly regulated by soil microorganisms, which convert soil nutrients, neutralize pollutants, and interact with each other [8][9][10]. The metabolites of soil microorganisms promote the dissolution of insoluble substances in soil; for example, phosphate-solubilizing bacteria can decompose P in phosphate ores, and potassium-solubilizing bacteria can decompose K in potassium ores to facilitate crop absorption and utilization [11,12]. Soil microorganisms are also involved in the decomposition and utilization of urea, and some fixate nitrogen by converting atmospheric N into fixed nitrides for plant use [13].…”

Section: Introductionmentioning

confidence: 99%

Coal‐mining subsidence changed distribution of the microbiomes and their functional genes in a farmland

et al. 2023

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Land subsidence is a serious geological event, and can trigger severe environmental and ecological issues. In this study, the influences of coalmining subsidence on distribution of farmland microbiomes and their functional genes were investigated by 16 S ribosomal RNA (rRNA) gene and metagenome sequencing. The results showed the existence of a core microbiome, which determined the community compositions across the subsidence farmland. Subsidence decreased the relative abundances of dominant Streptomyces, Nocardioides, and Rhizophagus, but increased the relative abundances of dominant Bradyrhizobium, Rhizobium, and Trichoderma. Subsidence also decreased the relative abundances of genes related to carbon metabolism, Quorum sensing, aminoacyl-transfer RNA (tRNA) biosynthesis, and oxidative phosphorylation, and increased the relative abundances of genes related to two-component system and bacterial chemotaxis. Furthermore, subsidence weakened the biosynthesis of organic carbons by decreasing the relative abundances of genes encoding glycosyl transferases, and strengthened decomposition of degradable organic carbons of the microbiomes and auxiliary activities by increasing the relative abundances of genes encoding glycoside hydrolases and polysaccharide lyases.The concentrations of total phosphorus, Mg 2+ , and Ca 2+ at the lower areas were significantly higher than those at the upper areas, indicating an associated loss of soil nutrients. Canonical correspondence analysis showed that soil moisture, pH, and the concentrations of NH 4 + and Ca 2+ were the main factors affecting the distribution of the microbiomes and their functional genes. Collectively, this study shows that coal-mining subsidence alters soil

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Distribution of Culturable Phosphate-Solubilizing Bacteria in Soil Aggregates and Their Potential for Phosphorus Acquisition

Cited by 20 publications

References 58 publications

Isolation and characterization of phosphate-solubilizing bacterium Pantoea rhizosphaerae sp. nov. from Acer truncatum rhizosphere soil and its effect on Acer truncatum growth

Isolation and characterization of phosphate-solubilizing bacterium Pantoea rhizosphaerae sp. nov. from Acer truncatum rhizosphere soil and its effect on Acer truncatum growth

Land reclamation increased labile and moderately labile P fractions and strengthened co‐occurrence network ofgcdcommunity in calcareous soils

Coal‐mining subsidence changed distribution of the microbiomes and their functional genes in a farmland

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