Distribution of D1 and D5 dopamine receptors in the primate nucleus accumbens

Muly, E. Chris; Maddox, Marcelia; Khan, Zafar U.

doi:10.1016/j.neuroscience.2010.06.025

Cited by 12 publications

(12 citation statements)

References 63 publications

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“…Immunohistochemistry and preparation for electron microscopy was done according to standard techniques (Muly et al, 2010). We used an affinity purified rabbit polyclonal antibody directed against the cytoplasmic domain of the GluR1 subunit of the AMPA glutamate receptor (ab1504; Millipore; Temicula, CA) used at 0.3 μg/ml and biotinylated goat-anti-rabbit secondary antisera (Vector, Burlingame, CA, USA) diluted 1:200.…”

Section: Methodsmentioning

confidence: 99%

Effects of stress on AMPA receptor distribution and function in the basolateral amygdala

Hubert

Rainnie

et al. 2013

Brain Struct Funct

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Stress is a growing public health concern and can lead to significant disabilities. The neural response to stressors is thought to be dependent on the extended amygdala. The basolateral amygdala (BLA) is responsible for associations of sensory stimuli with emotional valence and is thought to be involved in stress-induced responses. Previous behavioral and electrophysiological experiments demonstrate that, in response to stress, changes occur in glutamatergic neurotransmission within the BLA and, in particular in transmission at AMPA receptors. Given the established role of AMPA receptors in memory and synaptic plasticity, we tested the hypothesis that stress produces alterations in the distribution of these receptors in a way that might account for stress induced alterations in amygdala circuitry function. We examined the subcellular localization of GluR1 subunits of the AMPA receptor and the electrophysiological characteristics of BLA principal neurons in an animal model of unpredictable stress. Compared to controls, we demonstrated an increase in the ratio of labeled spines to labeled dendritic shafts in the BLA of rats 6 and 14 days post-stress, but not 1 day post-stress. Furthermore, the frequency of mini-EPSCs was increased in stressed animals without a change in general membrane properties, mini-EPSC amplitude, or in paired pulse modulation of glutamate release. Taken together, these data suggest that the shift of GluR1-containing AMPA receptors from dendritic stores into spines may be in part responsible for the persistent behavioral alterations observed following severe stressors.

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Section: Methodsmentioning

confidence: 99%

Effects of stress on AMPA receptor distribution and function in the basolateral amygdala

Hubert

Rainnie

et al. 2013

Brain Struct Funct

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“…There are differences within striatal regions in terms of the relative balance of D2 and D3 receptors (57), expression of the DA transporter (58), and DA D1 receptor density. (59) Moreover, there are not homogenous regions in terms of function. For example, DA D2/D3 receptors show opposite roles in subregions of the nucleus accumbens,(60) Finally, there is uncertainty regarding state-effects of illness and whether these are secondary to extremes of dietary intake, premorbid and persistent traits, or scars.…”

Section: Da Function In An and Bnmentioning

confidence: 99%

Does a Shared Neurobiology for Foods and Drugs of Abuse Contribute to Extremes of Food Ingestion in Anorexia and Bulimia Nervosa?

Kaye

Wierenga

Bailer

et al. 2013

Biological Psychiatry

142

101

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Is starvation in anorexia nervosa (AN) or overeating in bulimia nervosa (BN) a form of addiction? Alternatively, why are individuals with BN more vulnerable and AN protected from substance abuse? Such questions have been generated by recent studies that suggest that there are overlapping neural circuits for foods and drugs of abuse. In order to determine whether a shared neurobiology contributes to eating disorders (EDs) and substance abuse, this review focused on imaging studies that investigated response to tastes of food and tasks designed to characterize reward and behavioral inhibition in AN and BN. BN and those with substance abuse disorders may share dopamine D2 receptor related vulnerabilities, and opposite findings may contribute to “protection” from substance abuse in AN. Moreover, imaging studies provide insights into executive cortico-striatal processes related to extraordinary inhibition and self-control in AN and diminished inhibitory self-control in BN that may influence the rewarding aspect of palatable foods and likely other consummatory behaviors. AN and BN tend to have premorbid traits, such as perfectionism and anxiety that make them vulnerable to employing extremes of food ingestion which serve to reduce negative mood states. Dysregulation within and/or between limbic and executive cortio-striatal circuits contributes to such symptoms. Limited data support the hypothesis that reward and inhibitory processes may contribute to symptoms in ED and addictive disorders, but little is known about the molecular biology of such mechanisms in terms of shared or independent processes.

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“…Viral mediated silencing of targeted proteins offers higher temporal (RNAi starts right after transduction of targeted cells) and spatial control (inoculation of defined volumes and concentrations of viral vectors restricts silencing to targeted areas) of manipulations, compared to conventional knockout or pharmacological strategies. D1a (D1aR), rather than D1b receptors, are largely prevalent in striatal areas (Levey et al, 1993;Luedtke et al, 1999), including NAc (Muly et al, 2010). Therefore in the present study, D1aR silencing by lentiviral mediated expression of specific siRNA in discrete regions of rat NAc shell or core, was utilized to investigate the role of D1aR in cocaine reinforcement using an i.v.…”

Section: Introductionmentioning

confidence: 99%

Impairment of acquisition of intravenous cocaine self-administration by RNA-interference of dopamine D1-receptors in the nucleus accumbens shell

et al. 2015

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Microdialysis during i.v. drug self-administration (SA) have implicated nucleus accumbens (NAc) shell DA in cocaine and heroin reinforcement. However, this correlative evidence has not been yet substantiated by experimental evidence obtained by studying the effect of selective manipulation of NAc shell DA transmission on cocaine and heroin SA. In order to investigate this issue, DA D1a receptor (D1aR) expression was impaired in the NAc shell and core by locally infusing lentiviral vectors (LV) expressing specific D1aR-siRNAs (LV-siRNAs). Control rats were infused in the same areas with LV expressing GFP. Fifteen days later, rats were trained to acquire i.v. cocaine or heroin self-administration (SA). At the end of behavioral experiments, in order to evaluate the effect of LV-siRNA on D1aR expression, rats were challenged with amphetamine and the brains were processed for immunohistochemical detection of cFos and D1aR. Control rats acquired i.v. cocaine and heroin SA. Infusion of LV-siRNAs in the medial NAc shell reduced D1aR density and the number of c-Fos positive nuclei in the NAc shell, while sparing the core, and prevented the acquisition of cocaine, but not heroin SA. In turn, LV-siRNAs infusion in the core reduced D1aR density and the number of c-Fos positive nuclei in the same area, while sparing the shell, and failed to affect acquisition of cocaine. The differential effect of LV impairment of NAc shell D1aR on cocaine and heroin SA indicates that NAc shell DA acting on D1aR specifically mediates cocaine reinforcement.

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Distribution of D1 and D5 dopamine receptors in the primate nucleus accumbens

Cited by 12 publications

References 63 publications

Effects of stress on AMPA receptor distribution and function in the basolateral amygdala

Effects of stress on AMPA receptor distribution and function in the basolateral amygdala

Does a Shared Neurobiology for Foods and Drugs of Abuse Contribute to Extremes of Food Ingestion in Anorexia and Bulimia Nervosa?

Impairment of acquisition of intravenous cocaine self-administration by RNA-interference of dopamine D1-receptors in the nucleus accumbens shell

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