Distribution of different efflux pump genes in clinical isolates of multidrug-resistant Acinetobacter baumannii and their correlation with antimicrobial resistance

Lin, Ming-Chyuan; Lin, Yun-You; Tu, Chi-Chao; Lan, Chung-Yu

doi:10.1016/j.jmii.2015.04.004

Cited by 85 publications

(57 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The MacA-MacB-TolC is a three protein efflux system that expels out mainly macrolide class of antibiotics, and their expression may not be influenced in a large way by the eravacycline [61]. MATE family pumps are not much related to resistance towards the tetracycline class of drugs and basically confer resistance towards fluoroquinolones and imipenems [62]. However, there was a 7-fold change in the gene expression of MATE family pumps in the eravacycline induced A. baumannii ATCC 19606, but negative differential expression of negative 5-fold change in the JU0126 strain.…”

Section: Degs As Resistance Determinantsmentioning

confidence: 99%

Integrative analysis of outer membrane vesicles proteomics and whole-cell transcriptome analysis of eravacycline induced Acinetobacter baumannii strains

Kesavan

Vasudevan

et al. 2020

BMC Microbiol

View full text Add to dashboard Cite

Background: Acinetobacter baumannii is a multidrug-resistant (MDR) hazardous bacterium with very high antimicrobial resistance profiles. Outer membrane vesicles (OMVs) help directly and/or indirectly towards antibiotic resistance in these organisms. The present study aims to look on the proteomic profile of OMV as well as on the bacterial transcriptome upon exposure and induction with eravacycline, a new synthetic fluorocycline. RNA sequencing analysis of whole-cell and LC-MS/MS proteomic profiling of OMV proteome abundance were done to identify the differential expression among the eravacycline-induced A. baumannii ATCC 19606 and A. baumannii clinical strain JU0126. Results: The differentially expressed genes from the RNA sequencing were analysed using R package and bioinformatics software and tools. Genes encoding drug efflux and membrane transport were upregulated among the DEGs from both ATCC 19606 and JU0126 strains. As evident with the induction of eravacycline resistance, ribosomal proteins were upregulated in both the strains in the transcriptome profiles and also resistance pumps, such as MFS, RND, MATE and ABC transporters. High expression of stress and survival proteins were predominant in the OMVs proteome with ribosomal proteins, chaperons, OMPs OmpA, Omp38 upregulated in ATCC 19606 strain and ribosomal proteins, toluene tolerance protein, siderophore receptor and peptidases in the JU0126 strain. The induction of resistance to eravacycline was supported by the presence of upregulation of ribosomal proteins, resistance-conferring factors and stress proteins in both the strains of A. baumannii ATCC 19606 and JU0126, with the whole-cell gene transcriptome towards both resistance and stress genes while the OMVs proteome enriched more with survival proteins.Conclusion: The induction of resistance to eravacycline in the strains were evident with the increased expression of ribosomal and transcription related genes/proteins. Apart from this resistance-conferring efflux pumps, outer membrane proteins and stress-related proteins were also an essential part of the upregulated DEGs. However, the expression profiles of OMVs proteome in the study was independent with respect to the whole-cell RNA expression profiles with low to no correlation. This indicates the possible role of OMVs to be more of back-up additional protection to the existing bacterial cell defence during the antibacterial stress.

show abstract

Section: Degs As Resistance Determinantsmentioning

confidence: 99%

Integrative analysis of outer membrane vesicles proteomics and whole-cell transcriptome analysis of eravacycline induced Acinetobacter baumannii strains

Kesavan

Vasudevan

et al. 2020

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…The mechanisms of antibiotic resistance in A. baumannii are very complex, mainly involving the expression of active efflux pumps; changes in antibiotics targets; production of inactive enzymes; and reduction, deficiency, and mutation of outer membrane proteins (Lee et al, 2017). Overexpression of efflux pumps can decrease the accumulation of drugs and may be among the main mechanisms inducing A. baumannii multidrug resistance (Lin, Lin, Tu, & Lan, 2017;Yoon et al, 2015). To date, three Acinetobacter drug efflux (Ade) resistance-nodulation-cell division (RND) systems, AdeABC (Magnet, Courvalin, & Lambert, 2001), AdeFGH (Coyne, Rosenfeld, Lambert, Courvalin, & Périchon, 2010), and AdeIJK (Damier-Piolle et al, 2008), have been characterized in A. baumannii.…”

mentioning

confidence: 99%

Biofilm formation in Acinetobacter baumannii was inhibited by PAβN while it had no association with antibiotic resistance

Chen

Huang

et al. 2020

MicrobiologyOpen

View full text Add to dashboard Cite

This study was conducted to investigate the relationship between Acinetobacter baumannii biofilm formation and antibiotic resistance. Furthermore, the effects of PAβN, a potential efflux pump inhibitor, on A. baumannii biofilm formation and dispersion were tested, and the gene expression levels of efflux pumps were determined to study the mechanisms. A total of 92 A. baumannii isolates from infected patients were collected and identified by multiplex PCR. The antimicrobial susceptibility of A. baumannii clinical isolates was tested by VITEK 2 COMPACT®. Genotypes were determined by ERIC‐2 PCR. Biofilm formation and dispersion were detected by crystal violet staining. The presence and mRNA expression of efflux pump genes were analyzed by conventional PCR and real‐time PCR, respectively. More than 50% of the A. baumannii strains formed biofilm and were divided into different groups according to their biofilm‐forming ability. Antibiotic resistance rates among most groups did not significantly differ. There were 7 clonal groups in 92 strains of A. baumannii and no dominant clones among the different biofilm‐forming groups. PAβN inhibited A. baumannii biofilm formation and enhanced its dispersion, whereas adeB, adeJ, and adeG and the mRNA expression of adeB, abeM, and amvA showed no differences in the different biofilm‐forming groups. In conclusion, there was no clear relationship between biofilm formation and antibiotic resistance in A. baumannii. The effects of PAβN on A. baumannii biofilm formation and dispersion were independent of the efflux pumps.

show abstract

“…The antimicrobial drugs resistant due to adeM gene are not related to adeABC and totally known. In certain studies it was noted that adeM is not as-Journal of Biosciences and Medicines sociated with the resistance of β-lactams, or cephalosporin [73] [74]. But it could have an implication of resistance in that of amino glycosides, trimethoprim, fluoroquinolones, erythrocin, and chloramphenicol.…”

Section: Mate Efflux Pumpsmentioning

confidence: 99%

Mechanisms Efflux Pumps of <i>Acinetobacter baumannii</i> (MDR): Increasing Resistance to Antibiotics

Temgoua¹,

Wu²

2019

JBM

View full text Add to dashboard Cite

Acinetobacter baumannii has greatly increased its degree of resistance to become multidrug resistant (MDR) over the past 30 years and is on the red line of the most widely replicated bacteria according to World Health Organization (WHO). The efflux pumps are the main cause for the increasing antibiotic resistance of A. baumannii originated from nosocomial infection. The progressive resistance of A. baumannii even on the recent drugs (tigecycline and fosfomycin) reduces to very effective antibiotic scale. With attention focused on MDR and pan-drug-resistant (PDR) in A. baumannii multiple works on efflux pumps chemical inhibitor (NMP, PAβN, omeprazole, verapamil, reserpine, CCCP) are still in progress. Certain inhibitors from plants (Biricodar and timcodar, Falvone, Mahonia, Dalea versicolor, Lycopus europaeus, and Rosmarinus officinalis) have the capability to have such compounds according to their very significant synergistic effect with antibiotics. In this review we focused on the growth of antibiotic resistance to explain the mechanism of efflux pumps into these different super families and a comprehensive understanding of the extrusion, regulation and physiology role of drug efflux pumps in the essential development of anti-resistivity drugs. We recapitulated the evolution of the work carried out in these fields during the last years and in the course of elaboration, with the aim of increasing the chances of decreasing bacterial resistivity to antibiotics.

show abstract

Distribution of different efflux pump genes in clinical isolates of multidrug-resistant Acinetobacter baumannii and their correlation with antimicrobial resistance

Abstract: Efflux pump genes are universally present in the collected clinical MDRAB isolates. The upregulation of the adeB, adeJ, macB and abeS genes is more related with antibiotic resistance.

Cited by 85 publications

References 32 publications

Integrative analysis of outer membrane vesicles proteomics and whole-cell transcriptome analysis of eravacycline induced Acinetobacter baumannii strains

Integrative analysis of outer membrane vesicles proteomics and whole-cell transcriptome analysis of eravacycline induced Acinetobacter baumannii strains

Biofilm formation in Acinetobacter baumannii was inhibited by PAβN while it had no association with antibiotic resistance

Mechanisms Efflux Pumps of <i>Acinetobacter baumannii</i> (MDR): Increasing Resistance to Antibiotics

Contact Info

Product

Resources

About

Distribution of different efflux pump genes in clinical isolates of multidrug-resistant Acinetobacter baumannii and their correlation with antimicrobial resistance

Abstract: Efflux pump genes are universally present in the collected clinical MDRAB isolates. The upregulation of the adeB, adeJ, macB and abeS genes is more related with antibiotic resistance.

Cited by 85 publications

References 32 publications

Integrative analysis of outer membrane vesicles proteomics and whole-cell transcriptome analysis of eravacycline induced Acinetobacter baumannii strains

Integrative analysis of outer membrane vesicles proteomics and whole-cell transcriptome analysis of eravacycline induced Acinetobacter baumannii strains

Biofilm formation in Acinetobacter baumannii was inhibited by PAβN while it had no association with antibiotic resistance

Mechanisms Efflux Pumps of &lt;i&gt;Acinetobacter baumannii&lt;/i&gt; (MDR): Increasing Resistance to Antibiotics

Contact Info

Product

Resources

About

Mechanisms Efflux Pumps of <i>Acinetobacter baumannii</i> (MDR): Increasing Resistance to Antibiotics