Distribution of E.coli O157:H7 in fecal and urine samples of cattle

Al-Rudha, Aseel M H; Al-Rubaie, Ezdihar Mohammed Mahal; Khalil, N

doi:10.30539/iraqijvm.v40i1.142

Cited by 3 publications

(4 citation statements)

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“…This is higher than the study done in Debre-Birhan, Ethiopia (0.81%) [ 19 ], Hawassa, Ethiopia (4.7%) [ 34 ], Jimma, Ethiopia (7.3%) [ 48 ], Addis Ababa Municipal Abattoir, Ethiopia (6.4%) [ 49 ], Debre-Zeit, Ethiopia (7%) [ 50 ], Sudan (8%) [ 45 ], and Nigeria (0.2%) [ 51 ]. However, it is lower than the study reported in Iraqi (91.25%) [ 52 ], and Mexico (22.03%) [ 39 ]. These variations among different studies could be attributed to differences in geographical area, and the type of samples taken since recto-anal mucosal swab sample is more sensitive than fecal sampling for determining the prevalence of STEC O157:H7 in ruminant animals [ 6 ].…”

Section: Discussioncontrasting

confidence: 63%

“…Sudan (8%) [45], and Nigeria (0.2%) [51]. However, it is lower than the study reported in Iraqi (91.25%) [52], and Mexico (22.03%) [39]. These variations among different studies could be attributed to differences in geographical area, and the type of samples taken since recto-anal mucosal swab sample is more sensitive than fecal sampling for determining the prevalence of STEC O157:H7 in ruminant animals [6].…”

Section: Plos Onementioning

confidence: 93%

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Shiga toxin-producing Escherichia coli O157:H7 among diarrheic patients and their cattle in Amhara National Regional State, Ethiopia

Engda,

Tessema,

Mesifin

et al. 2023

PLoS ONE

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Background Shiga toxin-producing Escherichia coli O157:H7 (STEC O157:H7) is a zoonotic pathogen that causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome worldwide. This study aimed to determine the prevalence, antibiotic susceptibility, and associated risk factors of STEC O157:H7 among diarrheic patients and their cattle. Methods A cross-sectional study was conducted among diarrheic patients and their cattle in Amhara National Regional State, Ethiopia from December- 2020 to June- 2022. A total of 1,149 diarrheic patients and 229 cattle were included in the study. STEC O157:H7 detection was done using culture, latex agglutination test, and polymerase chain reaction on diarrheic stool samples and recto-anal mucosal swabs of cattle. Antibiotic susceptibility tests were performed using disk diffusion techniques. Risk factors association were identified using binary and multivariable logistic regression analysis. Results The overall prevalence of STEC O157:H7 in diarrheic patients and their cattle was 11.1% (128/1149) and 14.4% (33/229) respectively. High percentage of the study subjects were found in under-five children (34.5%). Age less than 5 (AOR: 4.02, 95%CI:1.608–10.058,P = 0.003), and greater than 64 years old (AOR:3.36, 95% CI:1.254–8.986, P = 0.016), presence of diarrheic patient in the house (AOR:2.11, 95%CI:1.309–3.390, P = 0.002), availability of cattle in the house (AOR:2.52, 95%CI:1.261–5.049, P = 0.009), and habit of consuming raw foods (AOR:4.35, 95%CI:2.645–7.148, P = 0.000) were risk factors. Antibiotic resistance was shown in 109(85.2%), and 31(93.9%) isolates from diarrheic patients and their cattle respectively. The highest levels of antibiotic resistance were found to tetracycline (54.7%, 69.7%) in diarrheic patients and their cattle respectively. Multiple drug resistance was also observed among 56(43.8%) and 11(33.3%) isolates in diarrheic patients and their cattle respectively. Conclusion Our study showed high prevalence of STEC O157:H7 in diarrheic patients and their cattle. Therefore, health education should be given to the community on how to care for animals, proper sanitation, and the impact of raw food consumption.

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Section: Discussioncontrasting

confidence: 63%

Section: Plos Onementioning

confidence: 93%

Shiga toxin-producing Escherichia coli O157:H7 among diarrheic patients and their cattle in Amhara National Regional State, Ethiopia

Engda,

Tessema,

Mesifin

et al. 2023

PLoS ONE

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“…Food-borne diarrhea, bloody diarrhea, and hemolytic uremic syndrome each account for around 4% of all infection cases in children. (Al-Rudha, 2016). Urinary tract infections occurring by E. coli O157:H7 are one of the most important illnesses in babies and children, and they are one of the leading causes of renal failure and mortality in patients (Al-Iraqi, 2017).…”

Section: Introductionmentioning

confidence: 99%

Study the Effect of Ciprofloxacin and Diclofenac Combination Treating Urinary Tract Infection by Resistance E.coli 0157:H7 in Female Rabbits

R Kbyeh,

N. Abedsalih

2023

AAVS

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This study is designed to highlight some of the anti-bacterial effect for diclofenac aganist resist. E .coli 0157: H7, as well as the ability of the diclofenac represented by anti-inflammatory and ciprofloxacin combat the exposure of urinary tract infection. The virulence components responsible for colonization and penetration also have a role in the pathogenicity of the resistant Escherichia coli 0157: H7 strain. Resistance Escherichia coli 0157 H7 for antibiotics decreases the cure rate. Repurposing Food and Drug Administration (FDA) approved medications against the contributing factors is a novel method to overcome this scarcity. The analgesic medication diclofenac was reported to have anti-virulence action against resistance Escherichia coli O157 :H7. This study aimed to demonstrate the antimicrobial effect of diclofenac, Ciprofloxacin, and a combination (diclofenac +ciprofloxacin) against clinical resistance E coli o157 H7 isolates by using Polymerase chain reaction (PCR). The presented study, all rabbits, forty-eighth adult female rabbit aged (6-8 weeks) and weighing between (1500 and 2000) gm., was randomly divided into six equal groups(8/each) groups injection 0.1 ml in 1 x 10 9 CFU Escherichia coli 0157: H7 by urinary catheterization route excepting in negative control and all groups resulted in a significant increase in urea creatinine, sodium, Potassium, chloride concentration in the blood after three days comparison with negative control and in all experimental period (14 days) which was no significant change (P < 0.05) between positive control (PC) and diclofenac treated groups but combination (ciprofloxacin 3.5 mg/kg+diclofenac1mg/kg) COM1 group and combination (ciprofloxacin 1.75 mg/kg+diclofenac1mg/kg) com2 significant decrease (P< 0.05) after (seven, fourteen) days comparison with positive control ,diclofenac group was used in combination with antibiotics as the anti-virulence agent which was enhance the ability of the immune system to eradicate infection. Altogether, we can conclude that the diclofenac has the required anti-boiflim effects to support ciprofloxacin effect and the body responses against urinary tract infection consequences of E coli O157 :H7

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“…In addition to infecting adults,as well as these organisms are also responsible for various outbreaks in animals, poultry, and humans around the world. These organisms cause food-borne diarrhea, urinary tract infection, bloody diarrhea, and hemolytic uremic syndrome, which occur with an average of 4% of infections in women and children (Khudhir et al, 2022).One of the most serious illnesses in babies and children which induce renal failure or result in patient death is urinary tract infection brought on by E. coli O157:H7 (Al-Rudha, 2016).…”

mentioning

confidence: 99%

Study the Antibacterial Mechanism of Diclofenac and its Activity Alone or Combined with Ciprofloxacin in Treating Urinary Tract Infection

Frah Razzaq Kbyeh,

Ahmed N. Abedsalih

2023

wjps

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This study aimed to compare the effects of diclofenac, ciprofloxacin and their combination groups on urine tract infection (U.T.I.s) caused by resistance E coli 0157 H7 in vitro and in vivo. Two hundred urine samples were collected from adult women patients suffering from U.T.I.s from February 2022 to July 2022. The urine specimen was cultured on the blood agar and then incubated at 37°C for (1-2) days; MacConkey agar is the media used for determining the biochemistry results, and identification by Rapidchek test which was performed on typical colonies from the selective plate. The M.I.C effect of Diclofenac began at (25600µg/ml) against resistance E coli 0157H7 while M.I.C of ciprofloxacin it was at (0.96mg/ml), but M.I.C of a combination between (diclofenacn+ciprofloxacin) it was at (400+0.48) mg/ml. Resistance induction assay,with the ciprofloxacin has increased four times, while with the diclofenac has doubled, whilst the combination between (diclofenacn+ciprofloxacin) has no changed after 21 days, according to M.I.C.in the time-kill assay, it was proved that the ciprofloxacin and the combination between (diclofenacn+ciprofloxacin) works after 6 hours, compared to the diclofenac, which started to reduce bacterial counting after 40. After that, the examination was carried out by scanning electronic microscope (S.E.M.) to exam the mechanics of diclofenac,work which was found that it was works as anti-biofilm In this study, forty-eighth (48) rabbits were used and divided into six groups as follow positive control (P.C.), negative control (N.C.), diclofenac (D.C.) in 1mg/kg group, ciprofloxacin (C.I.P.)in7mg/kg group, combination (ciprofloxacin 3.5 mg/kg+diclofenac1mg/kg)COM1 group and combination (ciprofloxacin 1.75 mg/kg+diclofenac1mg/kg) com2 All animals (injected with 0.1 ml in CFU E coli 0157 H7 by urinary catheterization route excepting negative control all group excepting negative control resulted in a significant increase in pus cell, epithelium cell concentration in the urine after three days E coli 0157 H7 infection comparison with negative control while in all experimental period after (14 days) showed no significant (P < 0.05) between positive control (P.C.) and diclofenac treated groups but combination COM1 group and com2 decrease significantly (P< 0.05) after (seven and fourteen) days comparison with positive control. diclofenac can be combined with antibiotics as an anti-virulence agent, enhancing the immune system's ability to eradicate infection

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Distribution of E.coli O157:H7 in fecal and urine samples of cattle

Cited by 3 publications

References 5 publications

Shiga toxin-producing Escherichia coli O157:H7 among diarrheic patients and their cattle in Amhara National Regional State, Ethiopia

Shiga toxin-producing Escherichia coli O157:H7 among diarrheic patients and their cattle in Amhara National Regional State, Ethiopia

Study the Effect of Ciprofloxacin and Diclofenac Combination Treating Urinary Tract Infection by Resistance E.coli 0157:H7 in Female Rabbits

Study the Antibacterial Mechanism of Diclofenac and its Activity Alone or Combined with Ciprofloxacin in Treating Urinary Tract Infection

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