Distribution of endogenous type B and type D sheep retrovirus sequences in ungulates and other mammals.

Abstract

The jaagsiekte sheep retrovirus (JSRV), which appears to be a type B/D retrovirus chimera, has been incriminated as the cause of ovine pulmonary carcinoma. Recent studies suggest that the sequences related to this virus are found in the genomes of normal sheep and goats. To

Jaagsiekte Sheep Retrovirus Proviral Clone JSRV JS7 , Derived from the JS7 Lung Tumor Cell Line, Induces Ovine Pulmonary Carcinoma and Is Integrated into the Surfactant Protein A Gene

“…The lesions were similar to those induced by clone JSRV 21 , a provirus which had been isolated directly from tumor DNA rather than from a cell line (25). Because both of these pathogenicity studies were performed only with animals that are known to contain at least 15 copies of endogenous sheep retroviruses closely related to JSRV (13) and because lesions were detected after a 4-to 5-month incubation period, the possibility that endogenous sheep retroviruses may contribute to JSRV pathogenicity through recombination cannot be excluded. Thorough sequence analysis of JSRV isolates recovered from animals inoculated with JSRV molecular clones will be required to investigate this possibility.…”

“…JSRV is very closely related to the enzootic nasal tumor virus which is associated with transmissible intranasal tumors of sheep and goats (6). JSRV is also closely related to a family of endogenous sheep retroviruses in the sheep genome (1,2,12,13,24). Using exogenous JSRV-specific U3 and TM probes, Southern blot hybridization revealed a single proviral integration site in the JS7 lung tumor cell line (1), suggesting this cell line as a good candidate for isolation of a JSRV provirus and characterization of its integration site.…”

Jaagsiekte Sheep Retrovirus (JSRV): from virus to lung cancer in sheep

“…A family of endogenous retroviruses, enJSRV (endogenous JSRV) closely related to JSRV, is present in domestic and wild sheep and goats [34]. JSRV and en-JSRV genomes are highly related with 90-98% homology in deduced amino-acid sequences [59].…”

Receptor Usage and Fetal Expression of Ovine Endogenous Betaretroviruses: Implications for Coevolution of Endogenous and Exogenous Retroviruses

“…JSRV induces ovine pulmonary adenocarcinoma (OPA), a contagious lung tumor of sheep originating from the differentiated epithelial cells of the distal tract of the lung (23). ENTV causes the enzootic nasal tumor of sheep and goats, wherein the tumor originates from the olfactory mucosa of the nasal or ethmoid turbinates (5-7).Fifteen to 20 copies of endogenous elements related to JSRV and ENTV (enJSRVs) are present in the genome of sheep, goats, and most wild members of the Ovis and Capra genera (11,12,29). Interestingly, enJSRVs are highly expressed along the epithelium of the genital tract of the ewe and, in particular, in the luminal and glandular epithelia of the endometrium of the uterus (20,21,26).…”