Distribution of ESBLs among Escherichia coli isolates from outpatients with recurrent UTIs and their antimicrobial resistance

Al-Mayahie, Sareaa Maseer Gatya; Kuriashy, Jaafar Jh Al

doi:10.3855/jidc.6661

Cited by 19 publications

(13 citation statements)

References 33 publications

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“…In addition, a similar result was obtained by Alqasim et al [25] in Saudi Arabia who reported that 41% of UPEC isolates were potential ESBL producers, but a higher rate of potential ESBL-producing UPEC was reported by Al-Mayahie et al [26] in Iraq, Thabit et al [27] in Egypt and Mukherjee et al [28] in India, where the rates were 80.2%, 76.47, 70%, respectively. For the ESBL confirmatory double-disk synergy test, DDST detected ESBLs in 42/110 (38.18%).…”

Section: Discussionsupporting

confidence: 76%

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Multiple Drug Resistance Patterns in Various Phylogenetic Groups of Hospital-Acquired Uropathogenic E. coli Isolated from Cancer Patients

et al. 2020

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Cancer patients are more susceptible to several bacterial infections, particularly urinary tract infections caused by uropathogenic E. coli (UPEC). The objective of this work was detection and the phylogenetic characterization of hospital-acquired isolates of uropathogenic E. coli in cancer patients and the determination of its relation with antibiotic resistance. A total of 110 uropathogenic E. coli responsible for hospital-acquired urinary tract infections in cancer patients were included in this study. A triplex PCR was employed to segregate different isolates into four different phylogenetic groups (A, B1, B2 and D). Drug resistance was evaluated by the disc diffusion method. All of the isolates were multiple drug-resistant (MDR) and 38.18% of all UPEC isolates were extended-spectrum beta-lactamase (ESBL) producers from which 52% were positive for the blaCTX-M gene, 40% for the blaTEM gene, and 17% for the blaSHVgene. Among 42 ESBL-producing uropathogenic E. coli isolates, the majority belonged to phylogenetic group B2 (43%), followed by group D (36%), group A (19%) and group B1 (2%). Our results have shown the emergence of MDR isolates among uropathogenic E. coli with the dominance of phylogenetic group B2. Groups A and B1 were relatively less common. The most effective drug in all phylogenetic groups was imipenem.

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Section: Discussionsupporting

confidence: 76%

“…Higher levels of ESBL production were reported by Al-Mayahie et al [26] in Iraq, Chandra et al [14] in India, Al-Agamy et al [30] in Egypt, Mekki et al [31] in Sudan, and Abayneh et al [32] in Southwest Ethiopia. The rates reported for each study were 64.8%, 62.5%, 60.9%, 53% and 76.5%, respectively.…”

Section: Discussionmentioning

confidence: 79%

Multiple Drug Resistance Patterns in Various Phylogenetic Groups of Hospital-Acquired Uropathogenic E. coli Isolated from Cancer Patients

et al. 2020

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“…From these tables we can observe that studies conducted in non-con ict affected countries appeared to be slightly more focused in terms of site of infection under investigation compared to studies conducted in con ict affected countries, where in a substantial proportion of cases the site of infection was not speci ed, or the authors were analysing resistance pro les of bacteria isolated from a miscellanea of specimens. Publication bias was assessed for a sub-sample of ten studies which presented the proportion of ESBL producers among E. coli isolates on urinary samples, six of which were conducted in con ict-affected countries (45,54,59,75,114,159) and four in non-con ict settings (154,155,164,165). Figure 4 shows the funnel plots elaborated on the logarithm of the proportion of ESBL producers amongst E. coli isolates in urine samples in all studies, and in studies conducted in con ict and non-con ict settings separately.…”

Section: Synthesis Of Resultsmentioning

confidence: 99%

Antimicrobial resistance among GLASS pathogens in conflict and non-conflict affected settings in the Middle East: A systematic review

Truppa

Abo‐Shehada

2020

Preprint

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Background: In spite of the evident general negative effects of armed conflict on countries’ health systems and populations’ health outcomes, little is known about similar impacts of conflicts on the spread of antimicrobial resistances (AMR). This review was to address this evidence gap and describe:1. Patterns of AMR in the Middle East (ME) and resistance profiles of pathogens included in the Global AMR Surveillance System (GLASS) supported by the World Health Organization;2. Differences in proportions of AMR isolates between conflict and non-conflict countries. Methods: A systematic literature review was conducted following PRISMA guidelines and searching five electronic databases. Subject heading and free text were searched for “antimicrobial resistances” and “Middle East”, to identify observational studies on AMR published from January 2011 to June 2018. Data were extracted from included articles on a predefined set of variables. Percentages of AMR were analysed as median and interquartile ranges. Risk of bias was assessed using the Newcastle-Ottawa Scale. Results: A total of 132 articles met the inclusion criteria. Included studies showed heterogeneity in study design, laboratory methods and standards for interpretation of results, and an overall high risk of bias. Main findings were the following:1. High proportions of carbapenem resistance in Acinetobacter spp. (median 74.2%: range), and both carbapenem resistance (median 8.1% and 15.4% for E. coli and K. pneumoniae respectively) and ESBL-production (median 32.3% and 27.9% for E. coli and K. pneumoniae respectively) amongst Enterobacteriaceae. S. aureus isolates showed a median methicillin resistance percentage of 45.1%, while vancomycin resistance was almost absent. A median of 50% of the strains of S. pneumoniae showed non-susceptibility to penicillin. 2. Similar trends were observed in conflict and non-conflict affected countries.Conclusions: There is a lack of standardization in the methodological approach to AMR research in the Middle East. The proportion of antibiotic resistances among specific GLASS pathogens is high, particularly among Acinetobacter spp.

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“…Recent studies also from Jordan and Middle East Arab region reported high prevalence of MDR E. coli recovered from community and hospitalized patients suffering of UTIs. The rates of MDR E. coli in these studies were ranged between 42% and 87.9% [ 20 , 22 - 26 ]…”

Section: Discussionmentioning

confidence: 99%

“…There are few studies which had investigated the incidence of CTX-M-types of ESBLs and specifically carbanpenemases among uropathogenic E. coli in the Middle East Arab countries [ 20 , 22 - 24 ]. This study has found high rates of MDR E. coli isolates carried CTX-M-1 (76%) and CTX-M-15 (78%).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Multidrug Resistant Uropathogenic E. Coli Isolates from Jordanian Patients

Nairoukh¹,

Mahafzah²,

Irshaid³

et al. 2018

TOMICROJ

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Background:Emergence of multi-drug resistant uropathogenic E. coli strains is an increasing problem to empirical treatment of urinary tract infections in many countries. This study investigated the magnitude of this problem in Jordan.Methods:A total of 262 E. coli isolates were recovered from urine samples of Jordanian patients which were suspected to have urinary tract infections (UTIs). All isolates were primarily identified by routine biochemical tests and tested for antimicrobial susceptibility by disc diffusion method. Fifty representative Multidrug Resistance (MDR) E. coli isolates to 3 or more antibiotic classes were tested for the presence of resistance genes of blaCTX-M- 1, 9 and 15, carbapenemase (blaIMP, blaVIM, blaNDM-1, blaOXA-48), fluoroquinolones mutated genes (parC and gyrA) and clone of ST131 type using PCR methods.Results:A total of 150/262 (57.3%) of E. coli isolates were MDR. Urine samples of hospitalized patients showed significantly more MDR isolates than outpatients. Fifty representative MDR E. coli isolates indicated the following molecular characteristics: All were positive for mutated parC gene and gyrA and for ST131 clone, and 78% were positive for genes of CTX-M-15, 76% for CTX-M-I and for 8% CTX-M-9, respectively. Additionally, all 50 MDR E. coli isolates were negative for carbapenemase genes (blaIMP, blaVIM, blaNDM-1, blaOXA-48), except of one isolate was positive for blaKPC-2 .Conclusion:This study indicates alarming high rates recovery of MDR uropathogenic E. coli from Jordanian patients associated with high rates of positive ST131 clone, fluoroquinolone resistant and important types of blaCTX-M.

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Distribution of ESBLs among Escherichia coli isolates from outpatients with recurrent UTIs and their antimicrobial resistance

Cited by 19 publications

References 33 publications

Multiple Drug Resistance Patterns in Various Phylogenetic Groups of Hospital-Acquired Uropathogenic E. coli Isolated from Cancer Patients

Multiple Drug Resistance Patterns in Various Phylogenetic Groups of Hospital-Acquired Uropathogenic E. coli Isolated from Cancer Patients

Antimicrobial resistance among GLASS pathogens in conflict and non-conflict affected settings in the Middle East: A systematic review

Molecular Characterization of Multidrug Resistant Uropathogenic E. Coli Isolates from Jordanian Patients

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