Distribution of Frozen-Thawed Spermatozoa in the Reproductive Tract of Gilts at Different Time Intervals After Insemination

Einarsson, S.; Viring, S.

doi:10.1530/jrf.0.0320117

Cited by 32 publications

(9 citation statements)

References 9 publications

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“…Questions remain as to how uterine contractions seen on B-mode images relate to uterine fluid movement. In gilts, the presence of seminal plasma causes delayed clearance of sperm from the uterus when compared to extender (Einarsson and Viring 1973) and our results suggest that the same may be true in mares.…”

Section: Uterine Contractilitysupporting

confidence: 62%

Effect of seminal plasma on uterine inflammation, contractility and pregnancy rates in mares

Portus

Reilas

Katila

2010

Equine Veterinary Journal

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Summary Reasons for performing study: There is conflicting evidence over the role seminal plasma plays in sperm transport and inflammation within the uterus of mares. In in vitro studies, seminal plasma has been shown to reduce polymorphonuclear neutrophil (PMN) function, but the opposite effect on uterine inflammation has been reported in vivo. Objectives: To study the effect of seminal plasma on uterine contractility, inflammation and pregnancy rates by inseminating mares with low doses of sperm free from seminal plasma (Group 1) and containing seminal plasma (Group 2). Methods: Synchronised mares were inseminated with 50 times 106 sperm in either skim milk extender or seminal plasma. Uterine lavage was performed 6 h after insemination to assess the inflammatory response. The contraction frequency of the uterus was measured over a 4 min period 10 mins and 6 h after insemination, using B‐mode ultrasonography. Pregnancy rates were assessed 16 days after insemination. Results: Uterine contractions were less frequent in Group 1 mares inseminated with seminal plasma and significantly more PMNs were found in the lavage fluid of those mares. Pregnancy rates were identical in both groups (62%). Conclusions: This study provides evidence that seminal plasma decreases uterine contractility and increases the inflammatory response of the uterus to semen. No effect of seminal plasma on pregnancy rates was demonstrated. Potential relevance: Mares that develop persistent mating‐induced endometritis may have inherently poor uterine contractility and impaired uterine clearance. The presence of seminal plasma during breeding may not be desirable in these mares. The role of seminal plasma in problem mares warrants additional study.

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Section: Uterine Contractilitysupporting

confidence: 62%

Effect of seminal plasma on uterine inflammation, contractility and pregnancy rates in mares

Portus

Reilas

Katila

2010

Equine Veterinary Journal

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“…The life span of frozen thawed spermatozoa is limited to only a few hours (EINARSSON and VIRING, 1973; and hence rapid transport of frozen-thawed spermatozoa to the oviducts may be advantageous when inseminations are done close to the time of ovulation. In A.I.…”

Section: Discussionmentioning

confidence: 99%

In vivo Fertilizing Capacity of Deep Frozen Boar Semen Packaged in Plastic Bags and Maxi‐Straws

Bwanga

Hofmo

Grevle

et al. 1991

Journal of Veterinary Medicine Series A

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Summary Pooled ejaculates from six fertile boars were frozen under controlled conditions in Teflon® FEP‐film plastic bags (5 ml) and maxi‐straws (2.5 ml) using 3 % glycerol as cryoprotectant. The percentages of both post‐thaw motility and normal apical ridges were significantly higher (P< 0.001) for the bags (54.5 and 75 %) than for the maxi‐straws (40.1 and 59.4 %) respectively. For evaluation of the in vivo fertilizing capacity of the frozen‐thawed spermatozoa, 26 gilts were inseminated once 24 h after the first observation of standing reflex in their second oestrus, with 5 ml of semen (containing 5 billion spermatozoa) reconstituted in 80 ml of BTS from either bags or maxi‐straws. Ova were recovered from the oviducts/uteri 2–4 days following insemination and examined for cleavage and sperm binding to the zona pellucida (ZP). Significantly higher rates (P < 0.02) of fertilized ova were found in the bag‐inseminated (75%) than in maxi‐straw inseminated gilts (63%); and similarly their ova had significantly more spermatozoa in the ZP, irrespective of whether they were fertilized or non‐fertilized. This study confirmed that the plastic bags are suitable and may be used for packaging single insemination doses of deep frozen boar semen for routine A. I. work.

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“…2005). A beneficial effect of thawing cryopreserved boar sperm in SP on in vivo sperm longevity (Einarsson and Viring 1973) and on gilt and sow fertility has also been documented (e.g. Crabo and Einarsson 1971; Einarsson et al.…”

Section: Introductionmentioning

confidence: 98%

Effect of Insemination–Ovulation Interval and Addition of Seminal Plasma on Sow Fertility to Insemination of Cryopreserved Sperm

Abad

Garcia

Sprecher

et al. 2007

Reprod Domestic Animals

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In swine, the use of frozen-thawed (FT) sperm for artificial insemination (AI) is limited because of poor sow fertility, possibly associated with a post-thaw capacitation-like status resulting in fewer fully viable sperm. Sow fertility to AI with FT sperm may improve with deeper deposition of sperm within the female tract, insemination very close to ovulation, or reversal of cryocapacitation by seminal plasma (SP). We performed two experiments to examine these suggestions. In experiment 1, 122 multiparous Yorkshire sows received 600 IU equine chorionic gonadotrophin at weaning and 5 mg pLH 80 h later to control time of ovulation. The predicted time of ovulation (PTO) was 38 h after pLH injection. Thereafter, sows were assigned on the basis of parity to a single AI of FT sperm at 2 h before PTO, or at 12 h before PTO, or FT sperm supplemented with 10% SP at 12 h before PTO. Control sows received fresh semen at 12 h before PTO. All semen doses were adjusted to 3 x 10(9) live cells and deposited into the cervix. Experiment 2 employed 99 multiparous crossbred sows and repeated the treatments of experiment 1 except that all FT inseminations were intrauterine. In both experiments, farrowing rates were lower (p < 0.01) following FT inseminations with no effect of time of insemination or of supplemental SP. In experiment 1, litter size was smaller following FT insemination (p < 0.05), but no effect on litter size was evident in experiment 2. Supplemental SP had no effect on litter size in either experiment. The lack of effect of either SP or timing of FT insemination on sow fertility suggests that the non-lethal sperm cryoinjury affecting fertility involves more than just cryocapacitation.

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Distribution of Frozen-Thawed Spermatozoa in the Reproductive Tract of Gilts at Different Time Intervals After Insemination

Cited by 32 publications

References 9 publications

Effect of seminal plasma on uterine inflammation, contractility and pregnancy rates in mares

Effect of seminal plasma on uterine inflammation, contractility and pregnancy rates in mares

In vivo Fertilizing Capacity of Deep Frozen Boar Semen Packaged in Plastic Bags and Maxi‐Straws

Effect of Insemination–Ovulation Interval and Addition of Seminal Plasma on Sow Fertility to Insemination of Cryopreserved Sperm

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