Distribution of G (VP7) and P (VP4) genotypes of group A bovine rotaviruses from Turkish calves with diarrhea, 1997–2008

Alkan, Feray; Özkul, Aykut; Oğuzoğlu, Tuba Çiğdem; Timurkan, Mehmet Ozkan; Caliskan, E.; Martella, Vito; Burgu, İbrahim

doi:10.1016/j.vetmic.2009.09.016

Cited by 49 publications

(74 citation statements)

References 39 publications

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“…These stud farms have an exchange procedure of horses for reproductive reasons especially. To date, there is no investigation based on the molecular characterization of VP4 and VP7 gene regions of GARVs from horses while the knowledge were reported about GARVs from other species in Turkey 31,32 . It's possible reasons are the inadequate knowledge on the rotavirus infection in foal diarrhea cases, disregarding of the mild diarrhea.…”

Section: Discussionmentioning

confidence: 99%

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Alkan

Timurkan²,

Karayel³

2013

Kafkas Univ Vet Fak Derg

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SummaryIn this study, the aetiological agent of diarrhea in Arabian thoroughbred foals housed in a stud farms and it's molecular characterization were reported. Out of sampled seven foals with diarrhoea, five were detected positive by RT-PCR depending on the amplification of the VP6 gene of rotavirus. Then the aetiological agent was characterized genetically by sequence analysis of the genome segments encoding VP6, VP7, VP4 and NSP4 of rotavirus. Findings revealed that the Turkish equine rotavirus circulating within this stud farm belongs to G3 and P[12] genotype with E2 NSP4 and I6VP6. This is the first study to report the G and P genotypes of equine group A rotaviruses in Turkey. Keywords: Rotavirus, Equine, Genotyping, Diarrhoea, Turkey Türkiye'de Bir Arap Atı İşletmesindeki Taylarda Rotavirus İshali Salgını ÖzetBu çalışmada, bir at yetiştiriciliği işletmesinde bulunan safkan arap taylarındaki ishal olgusunun etiyolojik ajanının tespiti ve moleküler karakterizasyonu bildirildi. İshal semptomlu yedi taydan sağlanan materyallerin beşinde RT-PCR tekniği ile rotavirus VP6 geni yönünden pozitiflik saptandı. Daha sonra enfeksiyona neden olan virusun VP6, VP7, VP4 ve NSP4 gen bölgelerini kodlayan gen bölgelerinin dizi analizi yapıldı. Elde edilen verilere dayanılarak söz konusu at yetiştiriciliği işletmesinde enfeksiyona neden olan ERV saha suşunun E2 NSP4 ve I6VP6 ile ilişkili G3P[12] genotipinde olduğu belirlendi. Bu çalışma, Türkiye'de atlardaki grup A rotavirusların G ve P genotiplerinin bildirildiği ilk çalışmadır.

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Section: Discussionmentioning

confidence: 99%

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Alkan

Timurkan²,

Karayel³

2013

Kafkas Univ Vet Fak Derg

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“…General recommendations regarding decrease of RVA diarrhea include management practices, especially good hygiene and sanitation procedures, as well as pathogen-specific interventions, such as the use of vaccine prophylaxis [14,29]. Immunoelectron microscopy of rotavirus particles aggregated by antigen-antibody interaction in bovine feces.…”

Section: Introductionmentioning

confidence: 99%

Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals

Catroxo¹,

Martins²

2018

Stomach Disorders

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Gastroenteritis caused by viruses is considered to be one of the most important diseases in livestock, being the main cause of morbidity and mortality in young animals, culminating in serious economic losses due to costs with prophylaxis and treatment, increased susceptibility of animals to secondary infections, developmental delay and death. Stressful factors may support the onset of illness. Several viral agents can cause gastroenteritis in various animal species. Rotaviruses are considered the main cause of enteric infections in various animals, including humans constituting important zoonosis. Due to genetic diversity and their ability to cross the species barrier, the coronaviruses infect many species. In cattle, they cause "Winter Dysentery" in adult animals and "Neonatal Diarrhea" in newborn calves. In swine, they are responsible for "Transmissible Gastroenteritis" and "Swine Epidemic Diarrhea." Equines infected with coronavirus also develop severe gastroenteritis. Bovine viral diarrhea (BVD) caused by a flavivirus of the genus Pestivirus is related to digestive and reproductive disorders, affecting any productive sector, are it cut, milk or confinement. Transmission electron microscopy is an indispensable tool in the diagnosis of viral gastroenteric infectious diseases. Negative staining is a simple, fast and efficient technique, being ideal for the detection of gastroenteric viruses, being easily visualized. The immunoelectron microscopy (IEM) technique allows increasing the sensitivity of virus detection where low concentrations of virus are aggregated so that they may be more easily seen. The immunolabeling with colloidal gold technique utilizes specific antibodies tagged with particles of colloidal gold to label the antigen antibody reaction. Embedding resin technique allows obtaining information on the virus-cell interaction. The different transmission electron microscopy modalities promotes a fast and accurate diagnosis of the different gastroenteric viral agents, allowing prophylactic measures of control and prevention in the creations to be promptly instituted, avoiding animal losses and disastrous economic losses, and collaborating with the National Porcine and Bovine Agribusiness.

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“…G6 and G10 are the predominant rotavirus serotypes in cattle in most (11)(12)(13)(14)(15)(16) but not all areas of the world (17). Bovine G6 strains were reported in many countries to be associated with P [1], P [5], and P [11] (11,12,(14)(15)(16).…”

mentioning

confidence: 99%

“…Bovine G6 strains were reported in many countries to be associated with P [1], P [5], and P [11] (11,12,(14)(15)(16). Two G8P [1] strains, NIC522 and B12, were described as evidence of direct transmission of bovine rotaviruses to humans (18,19).…”

mentioning

confidence: 99%

“…Two G8P [1] strains, NIC522 and B12, were described as evidence of direct transmission of bovine rotaviruses to humans (18,19). To date, no report has provided evidence for the direct transmission of the most common G6P [1], G6P [5], or G6P [11] bovine rotavirus strains to human children. There were, however, a few sporadic cases of G6 strains isolated from humans that were combined with P [6], P [9], and P [14] P types (reviewed by Martella et al [5]).…”

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confidence: 99%

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Identification by Full-Genome Analysis of a Bovine Rotavirus Transmitted Directly to and Causing Diarrhea in a Human Child

et al. 2013

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The genome of rotaviruses consists of 11 segments of double-stranded RNA, and each genome segment has multiple genotypes. Thus, the genotype constellation of an isolate is often indicative of its host species. Albeit rarely, interspecies transmission occurs either by virions with nonreassorted or reassorted genomic segments. A rotavirus with the G6P[1] genotype, Ro8059, was isolated from the stool of a 1-year-old child during routine characterization of diarrheal specimens from a sentinel clinic in Israel in 1995. Since genotype G6P[1] is generally associated with bovine rotaviruses, and the child developed diarrhea within days of his first contact with calves at an urban farm, the aim of this study was to characterize the whole genomic constellation of Ro8059 and four G6P[1] bovine strains, BRV101, BRV105, BRV106, and CR231/39, by RNA-RNA hybridization and full genome sequencing to determine whether some or all of the segments were of bovine origin. The genome constellations of all four bovine G6P[1] strains were G6-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3 for VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5, respectively. Ro8059 shared the same genotype constellation with these bovine strains, with high nucleotide sequence identities (95.84 to 100%) for each of the 11 segments indicating that Ro8059 represented a direct interspecies whole-genome transmission of a nonreassorted rotavirus from a calf to a human infant. We conclude that this was the earliest example with a complete epidemiological link in which an entirely bovine rotavirus directly infected a human child and caused a symptomatic diarrheal illness. Thus, not all bovine rotaviruses are always naturally attenuated to the human host.

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Distribution of G (VP7) and P (VP4) genotypes of group A bovine rotaviruses from Turkish calves with diarrhea, 1997–2008

Cited by 49 publications

References 39 publications

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Rotavirus Diarrhea Outbreaks in Arabian Thoroughbred Foals in a Stud Farm, Turkey

Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals

Identification by Full-Genome Analysis of a Bovine Rotavirus Transmitted Directly to and Causing Diarrhea in a Human Child

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