Distribution of GAD-immunoreactive neurons in the diencephalon of the African lungfishProtopterus annectens: Colocalization of GAD and NPY in the preoptic area

Trabucchi, Michèle; Chartrel, Nicolas; Pelletier, Georges; Vallarino, Mauro; Vaudry, Hubert

doi:10.1002/(sici)1096-9861(20000403)419:2<223::aid-cne6>3.0.co;2-1

Cited by 17 publications

(8 citation statements)

References 83 publications

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“…The intense expression of GAD65 and GAD67 mRNAs in neurons of the diencephalon, notably in the anterior preoptic area and to a lesser extent in the hypothalamus, suggests that GABA synthesized in these neurons could be involved in the neuroendocrine control of pituitary functions. In support of this hypothesis, we have previously observed the existence of a dense network of GAD‐immunoreactive fibers in the lungfish hypothalamus, terminating in the median eminence and in the neural lobe of the pituitary (Trabucchi et al,2000). Similarly, in the trout, GAD65‐containing nerve fibers originating from the preoptic region and the mediobasal hypothalamus directly project in the pars distalis of the pituitary (Anglade at al.,1999).…”

Section: Discussionmentioning

confidence: 64%

“…6). By using an antibody that recognizes preferentially the GAD65 isoform, we have previously shown that GAD immunoreactivity is located primarily in fibers and nerve endings but is much less abundant in cell bodies in the lungfish diencephalon (Trabucchi et al,2000). Taken together, these observations indicate that, in the lungfish, as in mammals, GAD65 and GAD67 isoforms may have a different subcellular distribution.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization and comparative localization of the mRNAs encoding two glutamic acid decarboxylases (GAD65 and GAD67) in the brain of the african lungfish, Protopterus annectens

Trabucchi

Trudeau

Drouin

et al. 2007

J of Comparative Neurology

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The existence of two distinct genes encoding two isoforms of glutamic acid decarboxylase (GAD65 and GAD67) has been demonstrated in most vertebrate classes, yet little is known about their differential distributions and functions in the central nervous system in nonmammalian vertebrates. In the present study, we have partially sequenced the cDNAs encoding GAD65 and GAD67 in the lungfish Protopterus annectens and determined their relative distributions in the adult brain by in situ hybridization histochemistry. The expression patterns of the GAD65 and GAD67 mRNAs were globally similar; the highest expression levels being observed in the granular layer of the olfactory bulb, the pallium, the subpallium, the anterior preoptic area, the thalamus, the hindbrain central gray, and the rhombencephalic visceral areas. However, striking differential expression was noticed in several structures. Very high to high concentrations of GAD67 mRNA were seen in the dorsal and ventral aspects of the anterior olfactory nucleus, which is in marked contrast to the very low expression of GAD65 in this region. Similarly, high levels of GAD67 mRNA were observed in the intermediate and ventral parts of the medial pallium that were virtually devoid of GAD65 mRNA. In contrast, GAD65 mRNA was found in the periaqueductal gray that did not express GAD67 mRNA. The differential expression of GAD65 and GAD67 mRNAs in these regions of the lungfish CNS indicates that the two GAD isoforms can be differentially regulated and that they may have distinct physiological roles.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Molecular characterization and comparative localization of the mRNAs encoding two glutamic acid decarboxylases (GAD65 and GAD67) in the brain of the african lungfish, Protopterus annectens

Trabucchi

Trudeau

Drouin

et al. 2007

J of Comparative Neurology

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“…The presence of this neuropeptidergic cell population, however, does not appear to be a conserved characteristic of ray‐finned fishes because it seems to be absent in many other species (García‐Fernández et al, 1992; Vecino and Ekström, 1992; Chiba and Honma, 1994; Chiba et al, 1996; Subhedar et al, 1996). In the Senegalese sole, as in goldfish (Kah et al, 1984), the nucleus posterioris periventricularis also contains catecholaminergic cells (Rodríguez‐Gómez et al, 2000b), whereas a co‐localization of NPY and GABA has been described in the preoptic area of the African lungfish (Trabucchi et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Neuropeptide Y (NPY) is a 36‐amino acid peptide, belonging to the pancreatic polypeptide molecular family that was originally isolated and characterized from the porcine brain (Tatemoto, 1982; Tatemoto et al, 1982), and later characterized in other vertebrates (Blomqvist et al, 1992). NPY is widely distributed in the CNS of dipnoans (Vallarino et al, 1995; Trabucchi et al, 2000), elasmobranchs (Vallarino et al, 1988; Chiba and Honma, 1992a), cyclostomes (Rawitch et al, 1992; Chiba et al, 1993), teleosts (Noe et al, 1989; Pontet et al, 1989; Danger et al, 1991; Chiba et al, 1996), amphibians (Danger et al, 1985; Cailliez et al, 1987; Perroteau at al., 1988), birds (Aste et al, 1991) and mammals (Smith et al, 1985; Bons et al, 1990). The extensive distribution in all vertebrate taxons, and the strong evolutionary conservation of the NPY gene (Blomqvist et al, 1992), suggest that this peptide plays important roles in the regulation of brain functions.…”

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confidence: 99%

“…In recent years, the number of papers describing the distribution of NPY in the brain and hypophysis of fish has increased (Vallarino et al, 1988; Pontet et al, 1989; Chiba and Honma, 1992a,b; García‐Fernández et al, 1992; Pickavance et al, 1992; Reiner and Northcutt, 1992; Vecino and Ekström, 1992; Chiba et al, 1993; Magliulo‐Cepriano and Schreibman, 1993; Chiba and Honma, 1994; Vecino et al, 1994; Zandbergen et al, 1994; Vallarino et al, 1995; Chiba et al, 1996; Subhedar et al, 1996; Trabucchi et al, 2000). To date, however, this type of study has not been undertaken in the pleuronectiform species, which represents one of the most evolved orders of euteleosts.…”

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Distribution of neuropeptide Y-like immunoreactivity in the brain of the senegalese sole (Solea senegalensis)

et al. 2001

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We present the results of an immunohistochemical study aimed at localizing the neuropeptide Y (NPY) in the brain of the Senegalese sole, Solea senegalensis, using an antiserum raised against porcine NPY and the streptavidin‐biotin‐peroxidase method. In this species, we have identified immunoreactive cells in the ventral and dorsal telencephalon, caudal preoptic area, ventrocaudal hypothalamus, optic tectum, torus longitudinalis, synencephalon and isthmic region. NPY‐immunoreactive fibers were profusely distributed throughout the brain, also reaching the adenohypophysis. The extensive distribution of NPY suggests an important role for this neuropeptide in a variety of physiological processes, including the neuroendocrine control of adenohypophyseal functions. Our results are compared with those obtained in other teleosts and discussed in relation to putative functions of NPY in the control of metabolism and reproduction in the Senegalese sole. Anat Rec 262:227–237, 2001. © 2001 Wiley‐Liss, Inc.

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Immunohistochemical localization of 3β‐hydroxysteroid dehydrogenase and 5α‐reductase in the brain of the African lungfish Protopterus annectens

Mathieu

Mensah‐Nyagan

Vallarino

et al. 2001

J of Comparative Neurology

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The localization of the enzymes responsible for the biosynthesis of neurosteroids in the brain of dipnoans has not yet been determined. In the present study, we investigated the immunohistochemical distribution of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 5 alpha-reductase (5 alpha-R) in the brain and pituitary of the African lungfish Protopterus annectens by using antibodies raised against type I human 3 beta-HSD and type I human 5 alpha-R. The 3 beta-HSD and 5 alpha-R immunoreactivities were detected in cell bodies and fibers located in the same areas of the lungfish brain, namely, in the pallium, thalamus, hypothalamus, tectum, and periaqueductal gray. Identification of astrocytes, oligodendrocytes, and neurons with antisera against glial fibrillary acidic protein, galactocerebroside and neurofilaments revealed that, in the lungfish brain, 3 beta-HSD immunolabeling is expressed exclusively by neurons, whereas the 5 alpha-R-immunoreactive material is contained in both neurons and glial cells. In the pituitary gland, 3 beta-HSD- and 5 alpha-R-like immunoreactivity was localized in both the pars distalis and the pars intermedia. The present study provides the first immunocytochemical mapping of two key steroidogenic enzymes in the brain and pituitary of a lungfish. These data strongly suggest that neurosteroid biosynthesis occurs in the brain of fishes, as previously shown for amphibians, birds, and mammals.

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Distribution of GAD-immunoreactive neurons in the diencephalon of the African lungfishProtopterus annectens: Colocalization of GAD and NPY in the preoptic area

Cited by 17 publications

References 83 publications

Molecular characterization and comparative localization of the mRNAs encoding two glutamic acid decarboxylases (GAD65 and GAD67) in the brain of the african lungfish, Protopterus annectens

Molecular characterization and comparative localization of the mRNAs encoding two glutamic acid decarboxylases (GAD65 and GAD67) in the brain of the african lungfish, Protopterus annectens

Distribution of neuropeptide Y-like immunoreactivity in the brain of the senegalese sole (Solea senegalensis)

Immunohistochemical localization of 3β‐hydroxysteroid dehydrogenase and 5α‐reductase in the brain of the African lungfish Protopterus annectens

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