Distribution of grafted β-cyclodextrin in porous particles for bone tissue engineering

Jacobsen, Peter Andreas Lund; Rafaelsen, Jens; Nielsen, Jeppe Lund; Juhl, Maria V.; Theilgaard, Naseem; Larsen, Kim Lambertsen

doi:10.1016/j.micromeso.2012.10.001

Cited by 5 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results obtained by DSC and XRD demonstrate that such structures are thermally stable and crystalline, increasing their chemical and physical stability. Furthermore, the increased specific surface area with the successful grafting of amine groups makes these microcylinders suitable for future use as therapeutic vehicles through the immobilization of, for example, anticancer agents for tumor therapy. , Alternatively, immobilization of growth factors , or biomolecules that mediate cellular adhesion are options for application of microcylinders in tissue engineering and regenerative medicine …”

Section: Results and Discussionmentioning

confidence: 99%

“…Importantly, electrospun fibers with different morphologies and surface porosity can be obtained in a simple manner using solvents with different volatilities. − Surface porosity has an important effect on the functional properties of microsized particles, especially in the development of drug-delivery systems. Porous particles have shown higher loading efficiencies compared to dense particles, largely due to their increased surface-area-to-volume ratio . Further, the porosity of a particle affects the drug release kinetics; by creating macroporosity, a higher initial release can be achieved, , while the presence of interconnected micropores leads to larger diffusion pathways, resulting in a more sustained release and diminished burst release profile. , …”

Section: Introductionmentioning

confidence: 99%

“…Porous particles have shown higher loading efficiencies compared to dense particles, largely due to their increased surface-area-to-volume ratio. 30 Further, the porosity of a particle affects the drug release kinetics; by creating macroporosity, a higher initial release can be achieved, 31,32 while the presence of interconnected micropores leads to larger diffusion pathways, resulting in a more sustained release and diminished burst release profile. 33,34 Considering the advantages of electrospinning and aminolysis for the preparation of microsized particles, we combined both techniques in a top-down approach for (i) production of PLLA-based electrospun fibers and (ii) generation of PLLAbased microsized cylinders that feature high specific surface area and with or without surface porosity.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Top-Down Approach for the Preparation of Highly Porous PLLA Microcylinders

Castro

Giudice

Vermonden

et al. 2016

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

A wide range of particles have been developed for different applications in drug-delivery, tissue engineering, or regenerative medicine. In contrast to traditional spherical particles, nonspherical (e.g., cylindrical) particles possess several structural and morphological advantages that make them attractive for specific applications. Here, we developed a top-down approach to process electrospun fibers into microsized cylinders (i.e., microcylinders) with high specific surface area and with or without surface porosity. To obtain these microcylinders, poly(l-lactic acid) (PLLA) solutions were subjected to electrospinning, followed by an aminolysis-based chemical scission procedure. The morphology, structure, and chemical composition of the microcylinders were then characterized. The specific surface area and surface porosity of the microcylinders were controlled by the volatility of the solvents, and their length was dependent on the duration of the aminolysis reaction. During aminolysis, the microcylinders became functionalized with amine groups, enabling potential further modifications by grafting with compounds containing desired chemical groups, for example, carboxyl, carbonyl, or hydroxyl functional groups. Additionally, the microcylinders showed in vitro biocompatible properties related to cell viability. These data demonstrate that PLLA microcylinders with high specific surface area, optional surface porosity, amine-based functional handles granting additional functionalization, and cytocompatible properties are candidate materials for future biomedical applications.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Top-Down Approach for the Preparation of Highly Porous PLLA Microcylinders

Castro

Giudice

Vermonden

et al. 2016

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…52 To prevent infections, CD has frequently been used as an orthopedic implant coating. 16,18,29,31,[53][54][55][56] Specifically, it has been used in conjunction with hydroxyapatite to promote osseointegration. 29,31,53,56 These applications were able to show sustained antibiotic release from CD over 150-300 h, 18,53 enhanced bacteriostatic activity and osteoblast cytocompatibility, 16,29 and were able to inhibit the growth of S. aureus over 28 days.…”

Section: Orthopedic Applicationsmentioning

confidence: 99%

Emerging technologies for long-term antimicrobial device coatings: advantages and limitations

Cyphert

Recum

2017

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

This work provides an overview, with advantages and limitations of the most recently developed antibacterial coating technologies, enabling other researchers in the field to more easily determine which technology is most advantageous for them to further develop and pursue. AbstractOver the past 20 years, the field of antimicrobial medical device coatings has expanded nearly 30-fold with technologies shifting their focus from diffusion-only based (short-term antimicrobial eluting) coatings to long-term antimicrobial eluting and intrinsically antimicrobial functioning materials. A variety of emergent coatings have been developed with the goal of achieving long-term antimicrobial activity in order to mitigate the risk of implanted device failure. Specifically, the coatings can be grouped into two categories: those that use antibiotics in conjunction with a polymer coating and those that rely on the intrinsic properties of the material to kill or repel bacteria that come into contact with the surface. This review covers both long-term drug-eluting and non-eluting coatings and evaluates the inherent advantages and disadvantages of each type while providing an overview of variety applications that the coatings have been utilized in.

show abstract

“…These biodegradable materials disintegrate and are removed from the body after completion of their functions . Biopolymers such as chitosan (CHT), agarose, β‐cyclodextrin, cellulose, starch, alginate, and hyaluronic acid have been used for tissue engineering applications . Introducing ionizable groups on the polymer backbone enables polymers to conduct electricity in a physiological environment through counter flow of ions when electrical potential is applied .…”

Section: Introductionmentioning

confidence: 99%

Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization

et al. 2018

View full text Add to dashboard Cite

Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micronanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049-0.0068 mS/m due to the uniform coating of sulfonated polymers on the micronanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal-like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite-like structures with cell bodies for ES-treated and positive control growth factor-treated groups. Immunofluorescent staining revealed the presence of neuronal markers including β3-tubulin, microtubule-associated protein 2, and nestin in response to ES.

show abstract

Distribution of grafted β-cyclodextrin in porous particles for bone tissue engineering

Cited by 5 publications

References 28 publications

Top-Down Approach for the Preparation of Highly Porous PLLA Microcylinders

Top-Down Approach for the Preparation of Highly Porous PLLA Microcylinders

Emerging technologies for long-term antimicrobial device coatings: advantages and limitations

Polymeric ionically conductive composite matrices and electrical stimulation strategies for nerve regeneration: In vitro characterization

Contact Info

Product

Resources

About