Distribution of H Type 1 and of H Type 2 Antigens of ABO Blood Group in Different Cells of Human Submandibular Gland

Abstract

We have examined the immunohistochemical distribution of H Type 1 and of H Type 2 substances of the ABO blood group system in human submandibular gland using either of the two anti-H monoclonal antibodies MAb 1E3 and MAb 3A5. MAb 3A5 was specific for H Type 2, and MAb 1E3 reacted with each of H Type 1-H Type 4 artificial antigens. We have developed a competitive inhibition method against H Type 2 and have obtained MAb 1E3, which is fairly specific for H Type 1 under certain conditions. Mucous cells from secretors were strongly stained by 1E3 and weakly by 3A5, whereas those from nonsecretors showed no reaction with 1E3 and 3A5. Serous cells from both secretors and nonsecretors were stained neither by 1E3 nor by 3A5. Striated and interlobular duct cells were strongly stained by 1E3 and by 3A5, regardless of the secretor status. These results indicated that the expressions of the H Type 1 and H Type 2 in different cell types of the submandibular gland were controlled by different genes. In addition, we have determined the acceptor specificity of two alpha(1,2)fucosyltransferases (H and Se enzymes) after transient expressions of the FUT1 and FUT2 in COS7 cells, and found that the H enzyme activity was similar for both Type 1 and Type 2 precursors, and that Se enzyme activity with the Type 1 precursor was higher than that with the Type 2 precursor. Expression of the H Type 1 antigen in mucous cells was found to be dependent on the Se gene, whereas expressions of the H Type 1 and H Type 2 antigens in striated and interlobular duct cells were dependent on the H gene. (J Histochem Cytochem 46:69-76, 1998)

Presence of H Type 3/4 Chains of ABO Histo-blood Group System in Serous Cells of Human Submandibular Gland and Regulation of Their Expression by the Secretor Gene (FUT2)

“…Ken Furukawa and Shin Yazawa (Department of Legal Medicine, Gunma University School of Medicine). The anti-H 1E3 has been demonstrated to be reactive with H Type 1-H Type 4 oligosaccharides and to be reactive with Fuc ␣ 1-2Gal ␤ disaccharide, using a series of synthetic oligosaccharides (Nakajima et al 1993), but to be reactive predominantly with the H Type 1 and H Type 2 antigens in situ in the submandibular gland, as shown in our previous study (Liu et al 1998). Submandibular glands from individuals with different ABO histo-blood groups were obtained from autopsy cases (five secretors and four nonsecretors from O blood group individuals and four secretors from A and four secretors from B blood group individuals).…”

An Alu -mediated large deletion of the FUT2 gene in individuals with the ABO-Bombay phenotype

“…Two distinct α(1,2)fucosyltransferases are known to be present in human tissues. One is the H gene (FUT1)-encoded α(1,2)fucosyltransferase (H enzyme), which regulates the expression of the H antigen on erythrocyte membranes, and the other is the Secretor gene (FUT2)-encoded α(1,2) fucosyltransferase (Se enzyme), which regulates the expression of the H antigen in the secretory fluids and digestive mucosa (Oriol et al 1986;Clausen and Hakomori 1989;Liu et al 1998aLiu et al , 1999a. The frequency of H enzyme deficiency (Bombay and paraBombay phenotypes) is very low (e.g., 1 in 13,000 Indians and 1 in 312,081 Germans; Bhatia and Sanghvi 1962;Wagner and Flegel 1997).…”

Extensive polymorphism of the FUT2 gene in an African (Xhosa) population of South Africa

“…The ABO histo-blood group substances, which are oligosaccharide antigens, are expressed not only on red blood cell membranes but also in the gastrointestinal tract and secretions (Clausen and Hakomori 1989;Liu et al 1998). The H antigen, which is a precursor of A and B antigens, is synthesized by α(1,2)fucosyltransferase.…”