Distribution of haptoglobin phenotypes in a Korean population, using the semi-automated PhastSystem

Abstract

SUMMARY.We have established a new phenotyping method for haptoglobin, based on sodium dodecyl sulphate±polyacrylamide gel electrophoresis using the PhastSystem (Pharmacia Biotech, Uppsala, Sweden), followed by immunoblotting for detection. We measured haptoglobin concentrations and determined the haptoglobin phenotypes of 316 healthy Koreans using this method: 31 (9´8%) were of Hp 1-1 type, 140 (44´3%) of Hp 2-1 type and 145 (45´9%) of Hp 2-2 type. The haptoglobin allele frequencies were calculated to be 0´32 for Hp 1 and 0´68 for Hp 2 . We were able to visualize up to 12 bands from the human Hp 2-2 polymeric series, with molecular weights in the range 171´9 10 3 to 802´2 10 3 . The reference range of serum haptoglobin concentrations obtained by the IFCC (International Federation of Clinical Chemistry) standard method was 0´27±2´14 g/L. The serum haptoglobin concentration in Koreans was similar to that of Caucasians, but the Hp 1 allele frequency was lower in Koreans. Our method could be used in clinical laboratories as a simple and practical method of haptoglobin phenotyping. In addition, the Hp 2-2 polymeric series could be used as high molecular weight standards.Haptoglobin is a glycoprotein that is expressed by a genetic polymorphism as three major phenotypes: Hp 1-1, Hp 2-1 and Hp 2-2.

Haptoglobin genotypic distribution (including Hp0allele) and associated serum haptoglobin concentrations in Koreans

“…4 However, electrophoresis phenotyping could not discriminate between hypohaptoglobinaemia and true anhaptoglobinaemia. In our study, Hp 0 Hp 2 , but not Hp 0 Hp 1 , was shown to be associated with hypohaptoglobinaemia.…”

Phenotypic polymorphism of haptoglobin: A novel risk factor for the development of infection in liver cirrhosis

“…Haptoglobin phenotypes were determined in a blinded fashion from serum samples. We modified the phenotyping method for haptoglobin established by Yang et al [21] based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of sera followed by immunoblotting. Haptoglobin molecules were separated and identified using 5%-10% gradient gels (Hoefer SG 15 gradient maker; San Francisco, CA).…”

Haptoglobin Polymorphisms Are Associated with Crohn’s Disease, Disease Behavior, and Extraintestinal Manifestations in Hungarian Patients

“…Analyses were performed on sera frozen at -70 • C. Hp concentration was measured using the nephelometric method on a Cobas Integra 700 (Roche, Basel, Switzerland). We modified the phenotyping method for Hp established by Yang and coworkers [17], based on sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) of sera followed by immunoblotting. Hp molecules were separated and identified using 5%-10% gradient gels (Hoefer SG 15 gradient maker; San Francisco, CA, USA).…”