Distribution of HBV subgenotypes in Ribeirão Preto, Southeastern Brazil: a region with history of intense Italian immigration

Chachá, Silvana Gama Florêncio; Gomes‐Gouvêa, Michele Soares; Malta, Fernanda de Mello; Ferreira, S. E. S.; Villanova, Márcia G.; Souza, Fernanda Fernandes; Teixeira, Andreza Corrêa; Passos, Afonso Dinis da Costa; Pinho, João Renato Rebello; Martinelli, Ana de Lôurdes Candolo

doi:10.1016/j.bjid.2017.01.011

Cited by 16 publications

(26 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A very similar prevalence was found by Mello and colleagues [54] in a survey on the phylogeography of HBV/F in Brazil, in which HBV/F2 was suggested as being the oldest HBV/F subgenotype, thus representing the original native HBV of Brazil [54]. HBV/F2 have been described in variable proportions across Brazil [16,39,55,56]. Here, HBV/F2 was mostly found in North and Northeast displaying high similarity with other Brazilian sequences and with sequences from Venezuela.…”

Section: Discussionsupporting

confidence: 65%

“…Phylogenetic analysis revealed that HBV/D2 and D3 isolates were genetically related to sequences from East Europe and Italy, respectively. HBV/D3 is highly prevalent in Italy [31,38] and have been described in populations of Italian ancestry in Brazil [20,36,39,40,41] and Argentina [42]. In our study, all Brazilian D3 clustered together, suggesting closer ancestral relationships (intragroup divergence of 0.005 ± 0.001, versus 0.007 ± 0.001 when compared to international sequences).…”

Section: Discussionsupporting

confidence: 62%

“…However, this might be due to the limited number of HBV/D3 full-length genomes assigned to Italy in Genbank that hindered more robust phylogenetic analyses. Despite this, combined evidence as historical background, partial HBV/D3 sequencing, and Y-chromosome heritage pointed out Italy as the most plausible source of HBV/D3 circulating in Brazil [36,39,41,44].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil

Lago

Espírito-Santo

Costa

et al. 2019

Viruses

View full text Add to dashboard Cite

Hepatitis B virus (HBV) subgenotypes may be related to clinical outcomes and response to antiviral therapy. Most Brazilian studies on HBV subgenotypes are restricted to some regions and to specific population groups. Here, we provide an insight about genetic diversity of HBV subgenotypes in 321 serum samples from all five geographical regions, providing a representative overview of their circulation among chronic carriers. Overall, HBV/A1 was the most prevalent subgenotype, being found as the major one in all regions except in South Brazil. Among HBV/D samples, subgenotype D3 was the most prevalent, found in 51.5%, followed by D2 (27.3%) and D4 (21.2%). D2 and D3 were the most prevalent subgenotypes in South region, with high similarity with European strains. D4 was found in North and Northeast region and clustered with strains from Cape Verde and India. For HBV/F, the most frequent subgenotype was F2 (84.1%), followed by F4 (10.1%) and F1 (5.8%), closely related with strains from Venezuela, Argentina and Chile, respectively. Phylogeographic analyses were performed using an HBV full-length genome obtained from samples infected with genotypes rarely found in Brazil (B, C, and E). According to Bayesian inference, HBV/B2 and HBV/C2 were probably introduced in Brazil through China, and HBV/E from Guinea, all of them mostly linked to recent events of human migration. In conclusion, this study provided a comprehensive overview of the current circulation of HBV subgenotypes in Brazil. Our findings might contribute to a better understand of the dynamics of viral variants, to establish a permanent molecular surveillance on the introduction and dispersion patterns of new strains and, thus, to support public policies to control HBV dissemination in Brazil.

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil

Lago

Espírito-Santo

Costa

et al. 2019

Viruses

View full text Add to dashboard Cite

show abstract

“…A recent study in Brazil showed that due to Italian colonization, the dominance of genotype D/D3 is observed (Paoli et al 2018). A similar study was performed before in the same region, and once again, genotype D/D3 was found to be the most relevant genotype (Chacha et al 2017).…”

Section: Molecular Epidemiology Of Hepatitis B Virus In Turkish Cypriotsupporting

confidence: 67%

Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

Sumer

Sayan

2019

Polish Journal of Microbiology

View full text Add to dashboard Cite

There is an increased demand for molecular and epidemiological information regarding Hepatitis B Virus (HBV) infection as the disease severity depends on these specifications. We have aimed to analyze nucleos(t)ide analogues (NA) resistance and typical HBsAg escape mutations with the dispersion of HBV genotype/subgenotype/HBsAg serotypes on overlapping pol/S gene regions in the Turkish population. Samples were collected in Northern Cyprus. Reverse transcriptase (rt) region between 80–250 amino acids were amplified. Typical HBsAg escape mutations were determined as HBIg escape (6.48%), vaccine escape (8.34%), HBsAg misdiagnosis (9.25%), and immune escape mutations (8.34%). NAs resistances were determined as primary (2.78%), partial (2.78%), and compensatory mutations (26.85%) in overlapping pol/S gene region. The study patients were predominantly infected with HBV genotype D/D1 (98%). However, the predominant HBsAg serotype was ayw2 (99%). The most common NA resistance mutation was rtQ215H/P/S (16.67%), however, for S gene the misdiagnosis mutations were observed most frequently (9.25%). We can conclude that HBV D/D1 is the dominant strain and ayw2 is the dominant serotype in the Turkish Cypriot. Cyprus is an island located in the Eastern Mediterranean region, and it is, therefore, a key location for human trafficking and immigration; as a result of this reputation, it is necessary to analyze HBV phylogenetically for local dynamics, and our results indicate that treatment naïve population is prone to these pol/S gene mutations. However, if HBV strains were also analyzed among Greek Cypriots too, this would enable a complete island survey. With this work, we believe that we have enlightened this subject for further research.

show abstract

“…Among the 161 included patients, genotype D was the most frequent (94 patients; 54%), genotype A was detected in 58 individuals (36.0%), and genotype F was detected in only nine patients (5.6%). HBV genotypes and sub-genotypes were identified by phylogenetic analysis of the sequences characterised together with reference sequences available in GenBank (file HBV_Pol_Sequences_Brazil_RP.sqn: KY809880–KY810045) ( Chachá et al 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases

Chachá

Gomes‐Gouvêa

Malta

et al. 2017

Mem. Inst. Oswaldo Cruz

Self Cite

View full text Add to dashboard Cite

BACKGROUND In Brazil, few studies have investigated the prevalence of infection with the precore (PC) and basal core promoter (BCP) mutants of the hepatitis B virus (HBV).OBJECTIVES This study aimed to analyse the frequency of PC and BCP mutations among patients infected with HBV and to evaluate the association between the variants and advanced hepatic disease.METHODS A total of 161 patients infected with HBV were studied. To identify PC and BCP mutations, a 501-bp fragment of HBV DNA was amplified and sequenced.FINDINGS PC and BCP regions from HBV strains were successfully amplified and sequenced in 129 and 118 cases, respectively. PC and BCP mutations were detected in 61.0% and 80.6% of the cases, respectively. The A1762T/G1764A variant was identified in 36.7% of the patients with grade 1 and 2 liver fibrosis (29/79) and in 81.8% of the patients with grade 3 and 4 liver fibrosis (9/11) (p < 0.01); in 76.9% of the patients with cirrhosis (10/13) and in 38.1% of the patients without cirrhosis (40/105) (p = 0.01); and in 77.8% of the patients with hepatocellular carcinoma (HCC) (7/9) and in 39.4% of the patients without HCC (43/109) (p = 0.03).MAIN CONCLUSIONS A high prevalence of HBV PC and BCP mutants was found. The A1762T/G1764A variant was independently associated with advanced forms of liver fibrosis, hepatic cirrhosis, and HCC.

show abstract

Distribution of HBV subgenotypes in Ribeirão Preto, Southeastern Brazil: a region with history of intense Italian immigration

Cited by 16 publications

References 31 publications

Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil

Genetic Diversity of the Hepatitis B Virus Subgenotypes in Brazil

Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

Basal core promoter and precore mutations among hepatitis B virus circulating in Brazil and its association with severe forms of hepatic diseases

Contact Info

Product

Resources

About