Distribution of HLA‐A, ‐B, ‐Cw, and ‐DRB1 alleles and haplotypes in an isolated Han population in Southwest China

Abstract

In this study, polymorphisms of human leukocyte antigen (HLA) class I (A, B, and Cw) and class II (DRB1) loci were analyzed in an isolated Han population living in Fengyandong in the Yunnan province of Southwest China (FYDH) using a high-resolution polymerase chain reaction-Luminex typing method. A total of 13 A, 26 B, 15 Cw, and 23 DRB1 alleles of HLA were found in FYDH. The frequencies of A*1101, A*0207, A*2402, B*4601, B*1502, Cw*0102, Cw*0801, DRB1*0901, and DRB1*1202 were >10%. The following haplotypes were common with frequencies >5%: three A-B, four Cw-B, two B-DRB1, two A-B-DRB1, three A-B-Cw, two B-Cw-DRB1, and two A-B-Cw-DRB1 phylogenetic tree and multidimensional scaling analysis based on HLA-A, -B, and -DRB1 allele frequencies of 18 Han populations suggested that FYDH was an isolated Han population, but the analytic result also provided a suggestion that FYDH was genetically related to Chinese Southern Han. According to the characteristics of the HLA allele and haplotype distributions and significantly reduced allelic and haplotypic diversity in FYDH, we deduced that genetic drift and/or selection and subsequent geographic isolation had influenced the distribution characteristics of the HLA gene in FYDH. In addition, significantly reduced allelic and haplotypic diversity in FYDH makes it an ideal homogenous population and very useful model for future investigations of issues related to immunogenetic diseases in the Han population.

A Conserved E7-derived Cytotoxic T Lymphocyte Epitope Expressed on Human Papillomavirus 16-transformed HLA-A2+ Epithelial Cancers

“…In the Korean population, A*0201 and A*0206, which are E7 [11][12][13][14][15][16][17][18][19] binding alleles, compose 85% of the A2 positive population (79). Among the 16 HLA-A2 alleles that are not predicted to bind E7 [11][12][13][14][15][16][17][18][19] , we find that A*0207, A*0278, A*9208 and A*9217 are found in the Chinese population, with the frequency of A*0207 higher than 10% in the southern Chinese Han population (80,81).…”

HLA polymorphism of the Zhuang population reflects the common HLA characteristics among Zhuang-Dong language-speaking populations

“…For example, the predominant A-C-B haplotype, A*02:07-C*01:02-B*46:01, was predominant in Vietnamese as well as in some southern Chinese populations, such as the Dai, Hani, Miao, Bouyei, Shui, Han in Yunnan, and Han in Guangdong, but existed at low frequencies in Chinese Hui, Japanese, and Koreans. Another predominant haplotype, A*11:01-C*08:01-B*15:02, was also predominant in Vietnamese and some Chinese populations, such as the Bulang, Dai, Hani, Shui, Han in Yunnan, and Han in Guangdong, who were located in southern China (Tokunaga et al, 1997;Middleton et al, 2004;Chen et al, 2007;Hong et al, 2007;Ogata et al, 2007;Trachtenberg et al, 2007;Hoa et al, 2008;Shi et al, 2008;2010a;2010b;Wen et al, 2008;Yao et al, 2009). Based on the HLA alleles, haplotypes, and neighbor-joining tree analysis, we concluded that the Zhuang is a typical southern Chinese population.…”

“…Nei's standard genetic distances among 18 populations were calculated based on HLA-A, HLA-B, and HLA-DRB1 allele frequencies and a neighbor-joining tree was constructed using Mega4.0 software (Tamura et al, 2007). Frequency data for other Asian populations were obtained from previous studies, as follows: Wa and Jinuo (Shi et al, 2008), Bouyei (Imanishi et al, 1992;Chen et al, 2007), Maonan (Ogata et al, 2007), Han in Taiwan (Wen et al, 2008), Han in Hongkong (Hardy et al, 1997), Han in Guangdong (Trachtenberg et al, 2007), Menba (Zhang et al, 2005), Tibetan (Lai et al, 1999;, Hui (Yu et al, 2002;Hong et al, 2007), Han in Beijing (Sun et al, 1997;Deng et al, 2005), Han in Northern (Yang et al, 2006), Dai (Shi et al, 2010a), Han in Yunnan (Yao et al, 2009), and Bulang and Hani (Shi et al, 2010b). Fig.…”

“…HLA-A, HLA-B, HLA-C, and HLA-DRB1 polymorphisms were detected using the Luminex multi-analyte profiling system (xMAP) with a WAKFlow HLA typing kit (Wakunaga, Hiroshima, Japan) as described before (Yao et al, 2009;Shi et al, 2010b). Genotype determination and data analyses were performed automatically using the WAKFlow typing software according to the manufacturer's instructions.…”

“…For the HLA-DRB1 locus, DRB1*15:01, DRB1*16:02, DRB1*14:01, DRB1*15:02, and DRB1*03:01 were at frequencies >10% and accounted for 73.7% of the total. All of the alleles in this population were common and existed in other East Asia populations, except a rare allele, DRB1*14:18 (0.014), which was only identified in the Han in Yunnan (0.005), Lahu (0.018) and Yao (0.024) in Yunnan province (Jia et al, 2002;Liu et al, 2006;Yao et al, 2009).…”